Thymol Blue sodium
Based on 1 Customer Validation
Thymol Blue sodium is an acid-base indicator used to indicate changes in pH. Thymol Blue sodium fades from red to yellow at pH 1.2 to 2.8 and from yellow to blue at pH 8.0 to 9.6. Quantitative detection is achieved through the absorption peak shift (435 nm/596 nm) of the UV-visible spectrum. Thymol blue sodium can be fixed in a silica gel matrix through sol-gel technology to form a solid-state sensor for in-situ pH measurement in the marine environment and acid-base monitoring in the biomedical field.
For research use only. We do not sell to patients.
- CAS No.: 62625-21-2
- Formula: C27H29NaO5S
- Molecular Weight:488.57
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Thymol Blue acid-base indicator protocol (solution state)[1]:
1. Solution preparation
Dissolve Thymol Blue sodium salt in ethanol (or water/ethanol mixed solvent) to prepare a 0.1% (w/v) stock solution.
2. pH response test
Take the solution to be tested and add 1-2 drops of Thymol Blue indicator:
Acidic conditions (pH<1.7) The solution turns red (HI- form).
Neutral to weakly alkaline (1.7≤pH≤8.9: the solution is yellow (intermediate state.
Strongly alkaline (pH>8.9): the solution is blue (I2- form).
3. Quantitative detection
Use a spectrophotometer to measure the absorbance at 435 nm (HI-) and 596 nm (I2-), calculate the ratio R=A596/A435, and calculate the pH value using the ionization formula.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 62625-21-2
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Appearance Solid
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Molecular Weight 488.57
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Formula C27H29NaO5S
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Color Yellow to brown
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SMILES
[Na].O=S1(=O)OC(C2=CC(=C(O)C=C2C)C(C)C)(C3=CC(=C(O)C=C3C)C(C)C)C=4C=CC=CC41
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 6.72 mg/mL (13.75 mM; ultrasonic and adjust pH to 4 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0468 mL | 10.2339 mL | 20.4679 mL | 51.1697 mL |
| 5 mM | 0.4094 mL | 2.0468 mL | 4.0936 mL | 10.2339 mL | |
| 10 mM | 0.2047 mL | 1.0234 mL | 2.0468 mL | 5.1170 mL |
Thymol Blue sodium Related Classifications
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