TKB245
Based on 1 Customer Validation
TKB245 is a highly potent SARS-CoV-2 Mpro inhibitor that effectively blocks SARS-CoV-2 replication in VeroE6 cells.
For research use only. We do not sell to patients.
- Purity: 98.98%
- CAS No.: 2892688-16-1
- Formula: C30H35F4N5O5S
- Molecular Weight:653.69
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | CC50 |
>100 μM
Compound: 5; TKB245
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Cytotoxicity against human HeLa cells expressing ACE2 and TMPRSS2
Cytotoxicity against human HeLa cells expressing ACE2 and TMPRSS2
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[PMID: 37756225] |
| HepG2 | CC50 |
>200 μM
Compound: 5; TKB245
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Cytotoxicity against human HepG2 cells
Cytotoxicity against human HepG2 cells
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[PMID: 37756225] |
| Vero C1008 | CC50 |
>100 μM
Compound: 5; TKB245
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Cytotoxicity against African green monkey Vero E6 cells in presence of P-glycoprotein inhibitor, CP100356
Cytotoxicity against African green monkey Vero E6 cells in presence of P-glycoprotein inhibitor, CP100356
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[PMID: 37756225] |
| Vero C1008 | CC50 |
>100 μM
Compound: 5; TKB245
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Cytotoxicity against African green monkey Vero E6 cells incubated for 3 days by WST-8 assay
Cytotoxicity against African green monkey Vero E6 cells incubated for 3 days by WST-8 assay
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[PMID: 37756225] |
Chemical Information
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CAS No. 2892688-16-1
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Appearance Solid
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Molecular Weight 653.69
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Formula C30H35F4N5O5S
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Color White to off-white
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SMILES
O=C([C@@H]1[C@@]2([H])C(C)(C)[C@@]2([H])CN1C([C@@H](NC(C(F)(F)F)=O)C(C)(C)C)=O)N[C@@H](C[C@H]3C(NCC3)=O)C(C4=NC5=C(F)C=CC=C5S4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (152.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.82 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5298 mL | 7.6489 mL | 15.2978 mL | 38.2444 mL |
| 5 mM | 0.3060 mL | 1.5298 mL | 3.0596 mL | 7.6489 mL | |
| 10 mM | 0.1530 mL | 0.7649 mL | 1.5298 mL | 3.8244 mL | |
| 15 mM | 0.1020 mL | 0.5099 mL | 1.0199 mL | 2.5496 mL | |
| 20 mM | 0.0765 mL | 0.3824 mL | 0.7649 mL | 1.9122 mL | |
| 25 mM | 0.0612 mL | 0.3060 mL | 0.6119 mL | 1.5298 mL | |
| 30 mM | 0.0510 mL | 0.2550 mL | 0.5099 mL | 1.2748 mL | |
| 40 mM | 0.0382 mL | 0.1912 mL | 0.3824 mL | 0.9561 mL | |
| 50 mM | 0.0306 mL | 0.1530 mL | 0.3060 mL | 0.7649 mL | |
| 60 mM | 0.0255 mL | 0.1275 mL | 0.2550 mL | 0.6374 mL | |
| 80 mM | 0.0191 mL | 0.0956 mL | 0.1912 mL | 0.4781 mL | |
| 100 mM | 0.0153 mL | 0.0765 mL | 0.1530 mL | 0.3824 mL |
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