TMBIM6 antagonist-1

Name: TMBIM6 antagonist-1
Brand: MedChemExpress
SKU: HY-137175
Rating: 4.5 (1 reviews)

Cat. No.: HY-137175 Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

TMBIM6 antagonist-1, a potential TMBIM6 antagonist, prevents TMBIM6 binding to mTORC2, decreases mTORC2 activity, and also regulates TMBIM6-leaky Ca²⁺.

For research use only. We do not sell to patients.

TMBIM6 antagonist-1 Chemical Structure

CAS No. : 123134-61-2

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1750	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

TMBIM6 antagonist-1, a potential TMBIM6 antagonist, prevents TMBIM6 binding to mTORC2, decreases mTORC2 activity, and also regulates TMBIM6-leaky Ca²⁺^[1].

In Vitro

TMBIM6 antagonist-1 (BIA, 0.5-10 μM, 3 days) significantly and dose-dependently inhibits cell viability in HT1080, MCF7, MDA-MB-2341 and SKBR3 cells, with IC₅₀ values of 1.7 ± 0.1 μM for HT1080, 2.6 ± 0.4 μM for MCF cells, 2.6 ± 0.5 μM for MDA-MB-231 cells, and 2.4 ± 0.4 μM for SKBR3 cells, respectively^[1].
TMBIM6 antagonist-1 (BIA, 10 μM) treatment decreases cell migration in HT1080, MCF7, MDA-MB-231, and SKBR3 cells, not TMBIM6 KO HT1080 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HT1080, MCF7, MDA-MB-2341 and SKBR3 cells.
Concentration:	0.5-10 μM.
Incubation Time:	3 days.
Result:	Inhibited cell viability.

Western Blot Analysis^[1]

Cell Line:	WT and TMBIM6 KO HT1080 cells.
Concentration:	0, 2, 5 μM.
Incubation Time:
Result:	Downregulated the protein levels of AKT-pS473.

In Vivo

TMBIM6 antagonist-1 (1 mg/kg, IP 5 days per week during 25 days) significantly impaires cell-driven tumor growth^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to eight-week BklNbt:BALB/c/nu/nu old mice (HT1080 and MDA-MB-231 cells)^[1].
Dosage:	1 mg/kg.
Administration:	IP 5 days per week during 25 days.
Result:	Impaired cell-driven tumor growth.

Molecular Weight

268.27

Formula

C₁₅H₁₂N₂O₃

CAS No.

123134-61-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC=CC=C1N)/C=C/C2=CC=CC([N+]([O-])=O)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (186.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7276 mL	18.6379 mL	37.2759 mL
5 mM	0.7455 mL	3.7276 mL	7.4552 mL
10 mM	0.3728 mL	1.8638 mL	3.7276 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (273 KB) SDS (0 KB)

COA (246 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Hyun-Kyoung Kim, et al. TMBIM6/BI-1 contributes to cancer progression through assembly with mTORC2 and AKT activation. Nat Commun. 2020 Aug 11;11(1):4012. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7276 mL	18.6379 mL	37.2759 mL	93.1897 mL
	5 mM	0.7455 mL	3.7276 mL	7.4552 mL	18.6379 mL
	10 mM	0.3728 mL	1.8638 mL	3.7276 mL	9.3190 mL
	15 mM	0.2485 mL	1.2425 mL	2.4851 mL	6.2126 mL
	20 mM	0.1864 mL	0.9319 mL	1.8638 mL	4.6595 mL
	25 mM	0.1491 mL	0.7455 mL	1.4910 mL	3.7276 mL
	30 mM	0.1243 mL	0.6213 mL	1.2425 mL	3.1063 mL
	40 mM	0.0932 mL	0.4659 mL	0.9319 mL	2.3297 mL
	50 mM	0.0746 mL	0.3728 mL	0.7455 mL	1.8638 mL
	60 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5532 mL
	80 mM	0.0466 mL	0.2330 mL	0.4659 mL	1.1649 mL
	100 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9319 mL