TNP-351

Cat. No.: HY-19095: FAQs
Data Sheet Handling Instructions

Molarity Calculator

TNP-351 is an antifolate. TNP-351, a dihydrofolate reductase (DHFR) inhibitor, has potent antitumor activity against not only leukemia cells but also solid tumor cells in vitro and in vivo.

For research use only. We do not sell to patients.

TNP-351 Chemical Structure

CAS No. : 125991-51-7

Size		Stock
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

TNP-351 is an antifolate. TNP-351, a dihydrofolate reductase (DHFR) inhibitor, has potent antitumor activity against not only leukemia cells but also solid tumor cells in vitro and in vivo^[1].

IC₅₀ & Target

Antifolate, DHFR^[1]

In Vitro

TNP-351 inhibits the proliferation of mouse L1210 leukemia cells and human CCRF-CEM lymphoblastic leukemia cells with ED₅₀ values of 0.79 and 2.7 nM, respectively^[1].
The ED₅₀ values determined for the parent cell line CCRF-CEM, CCRFCEM R30/6 subline, CCRF-CEM R1, and CCRF-CEM R2 are 2.7, 5.8, 94 and 76 nM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	L1210 and CCRF-CEM cells
Concentration:	0.1, 0.3, 1, 3, and 10 nM for L1210 cells; 0.3, 1, 3, 10, and 30 for CCRF-CEM cells
Incubation Time:	48 hours for L1210 cells; 72 hours for CCRF-CEM cells
Result:	The ED₅₀ values were 0.79 and 2.7 nM for L1210 and CCRF-CEM cells, respectively.

Molecular Weight

440.45

Formula

C₂₁H₂₄N₆O₅

CAS No.

125991-51-7

SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(CCCC2=CNC3=NC(N)=NC(N)=C32)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. F Itoh ,et al. Novel pyrrolo[2,3-d]pyrimidine Antifolate TNP-351: Cytotoxic Effect on Methotrexate-Resistant CCRF-CEM Cells and Inhibition of Transformylases of De Novo Purine Biosynthesis. Cancer Chemother Pharmacol. 1994;34(4):273-9. [Content Brief]

TNP-351 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TNP-351125991-51-7TNP351TNP 351AntifolateleukemiaantifolatedihydrofolatereductaseDHFRsolidtumorInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks