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Cat. No.: HY-187099

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TopoII/Tubulin-IN-3

TopoII/Tubulin-IN-3 is a Tubulin and Topoisomerase II inhibitor. TopoII/Tubulin-IN-3 induces G2 and M phase cell cycle arrest and promotes cell Apoptosis. TopoII/Tubulin-IN-3 exhibits anticancer activity against breast adenocarcinoma and liver cancer. TopoII/Tubulin-IN-3 can be used in studies related to breast adenocarcinoma and liver cancer.

For research use only. We do not sell to patients.

Formula: C₂₂H₁₉N₅O₄
Molecular Weight:417.42

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

TopoII/Tubulin-IN-3 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
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Biological Activity

Description

IC₅₀ & Target

^[1]

Topoisomerase II

All Topoisomerase Isoforms :

In Vitro

TopoII/Tubulin-IN-3 (Compound 26) potently inhibits the proliferation of MCF7 cells (IC₅₀ = 2.4 μM), moderately inhibits the proliferation of HepG2 cells (IC₅₀ = 6.3 μM), weakly inhibits the proliferation of HCT116 cells (IC₅₀ = 63.4 μM), and shows no significant cytotoxicity against normal BJ1 cells^[1].
TopoII/Tubulin-IN-3 inhibits tubulin polymerization in MCF7 cells by 75.0%^[1].
TopoII/Tubulin-IN-3 inhibits Topo II activity in MCF7 cells by 43.7%^[1].
TopoII/Tubulin-IN-3 (2.4 μM) induces G₂/M phase arrest in MCF7 cells^[1].
Treatment with TopoII/Tubulin-IN-3 (2.4 μM; 48 h) induces apoptosis in 41.26% of MCF7 cells, with 7.58% undergoing early apoptosis and 28.16% undergoing late apoptosis, and results in an extremely low necrosis rate (only 5.51%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

MCF7
Concentration:

2.4 μM
Incubation Time:

48 h
Result:

Induced total apoptosis in 41.26% of cells, with 7.58% in early apoptosis and 28.16% in late apoptosis.
Induced necrosis in only 5.51% of cells, compared to 3.04% total apoptotic cells in untreated control.

Chemical Information

Molecular Weight 417.42
Formula C₂₂H₁₉N₅O₄
SMILES

N#CC1=C(C2=CC(OC)=C(OC)C(OC)=C2)C=C(C3=CC=C(OC)C=C3)N4C1=NN=N4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hassan ES, et al. Nicotinonitrile based dual inhibitors of tubulin and topoisomerase II exhibit potent anticancer activity. Scientific reports. 2026 Jun 25;16(1):19595. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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