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Cat. No.: HY-100025

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HLCL-61

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

HLCL-61 is a first-in-class small-molecule inhibitor of protein arginine methyltransferase 5 (PRMT5).

For research use only. We do not sell to patients.

CAS No.: 586395-74-6
Formula: C₂₃H₂₄N₂O
Molecular Weight:344.45

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

HLCL-61 Chemical Structure

CAS No.: 586395-74-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

HLCL-61 is a first-in-class small-molecule inhibitor of protein arginine methyltransferase 5 (PRMT5).

IC₅₀ & Target

Protein arginine methyltransferase 5^[1]

All Histone Methyltransferase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MV4-11	IC₅₀	7.21 μM Compound: 98; HLCL-61	Inhibition of cell growth in human MV4-11 cells measured after 48 hrs by MTS assay Inhibition of cell growth in human MV4-11 cells measured after 48 hrs by MTS assay	[PMID: 38359536]
THP-1	IC₅₀	3.98 μM Compound: 98; HLCL-61	Inhibition of cell growth in human THP-1 cells measured after 48 hrs by MTS assay Inhibition of cell growth in human THP-1 cells measured after 48 hrs by MTS assay	[PMID: 38359536]

In Vitro

HLCL-61 (1-100 μM; 24-72 hours) reduces cells growth at dose-dependent manner with IC₅₀s of 14.12, 16.74, 6.3, 8.72 μM for MV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively^[1].
HLCL-61 shows effective inhibition of symmetric arginine dimethylation (me2) of histones H3 and H4 in AML samples, starting at 12 h post-treatment and persisting after 48 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MV4-11 cells, THP-1 cells, FLT3-WT blast (primary blasts from patients), FLT3-ITD blast (primary blasts from patients)
Concentration:

1, 5, 10, 25, 50, 100 μM
Incubation Time:

24, 48, 72 hours
Result:

Dose-dependent reduction in cell viability with IC50s of 14.12, 16.74, 6.3, 8.72 μM for MV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively.

Chemical Information

CAS No. 586395-74-6
Molecular Weight 344.45
Formula C₂₃H₂₄N₂O
SMILES

COC1=CC=CC=C1CNCC2=CC3=C(C=C2)N(CC)C4=C3C=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (2)

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Journal Impact Factor

Proc Natl Acad Sci U S A

MGMT-activated DUB3 stabilizes MCL1 and drives chemoresistance in ovarian cancer. [Abstract]

2019 Feb 19;116(8):2961-2966. PMID: 30718431
J Virol

PRMT5 Facilitates Infectious Bursal Disease Virus Replication through Arginine Methylation of VP1. [Abstract]

2023 Mar 30;97(3):e0163722. PMID: 36786602

HLCL-61 purchased from MedChemExpress. Usage Cited in: J Virol. 2023 Mar 30;97(3):e0163722. [Abstract]

HLCL-61 hydrochloride (4 µg/mL; 6 h) noticeably inhibits arginine methylation of VP1 in Flag-VP1-transfected DF-1 cells.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tarighat SS, et al. The dual epigenetic role of PRMT5 in acute myeloid leukemia: gene activation and repression via histone arginine methylation. Leukemia. 2016 Apr;30(4):789-99. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (2)
Purity & Documentation
References

1/16
1/16

HLCL-61 purchased from MedChemExpress. Usage Cited in: J Virol. 2023 Mar 30;97(3):e0163722. [Abstract]

Customer Validation
HLCL-61 hydrochloride (4 µg/mL; 6 h) noticeably inhibits arginine methylation of VP1 in Flag-VP1-transfected DF-1 cells.

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HLCL-61

Publications Citing Use of MedChemExpress (MCE) HLCL-61

All Histone Methyltransferase Isoforms