Pladienolide B
Based on 5 publication(s) in Google Scholar
Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3B1 subunit of the spliceosome. Pladienolide B exerts antitumor activities mediated through the inhibition of pre-mRNA splicing. Pladienolide B induces apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.29%
- CAS No.: 445493-23-2
- Formula: C30H48O8
- Molecular Weight:536.70
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pladienolide B
More
Customer Validation & Images
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RT-PCR
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WB
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
Pladienolide B (0.1-2 nM; 24-72 hours) inhibits human cervical carcinoma cells viability[3].
Pladienolide B (0.1-2 nM; 24-48 hours) reduces SF3b1 expression in human cervical carcinoma cells[3].
Pladienolide B induces (0.1-2 nM; 24 hours) cell cycle arrest and apoptosis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:0.1, 0.5, 1, 1.5, 2 nM
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Incubation Time:24, 48, 72 hours
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Result:Significantly decreased cell viability, and the decrease was concentration- and time-dependent.
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Cell Line:HeLa cells
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Concentration:0.1, 0.5, and 2 nM
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Incubation Time:24 hours
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Result:The apoptotic cells were highly induced at 24 hours.
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Cell Line:HeLa cells
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Concentration:0.1, 0.5, and 2 nM
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Incubation Time:24, 48 hours
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Result:Induced a time- and concentration-dependent decrease in cellular SF3b1 proteins.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female or male BALB/c nu/nu mice (7 weeks of age) (PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts)[4]
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Dosage:2.5, 5, and 10 mg/kg
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Administration:I.v.; daily for 5 days
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Result:Showed strong growth inhibitory or regressive activities against these xenografts.
Chemical Information
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CAS No. 445493-23-2
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Appearance Solid
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Molecular Weight 536.70
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Formula C30H48O8
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Color White to off-white
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SMILES
O=C1O[C@H](/C(C)=C/C=C/[C@@H](C)C[C@H]2O[C@@H]2[C@H](C)[C@@H](O)CC)[C@@H](C)/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Commun
YTHDF2 regulates self non-coding RNA metabolism to control inflammation and tumorigenesis. [Abstract]2025 Nov 12;16(1):9946. PMID: 41224760
Pladienolide B purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 12;16(1):9946. [Abstract]
The splicing inhibitor Pladienolide B (500 nM, 3 h) reduced the expression of pro-inflammatory cytokines in control and YTHDF2 knockdown cells, and also reduced the level of U6 snRNA.
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Mol Oncol
CDK11 inhibition induces cytoplasmic p21WAF1 splice variant by p53 stabilisation and SF3B1 inactivation. [Abstract]2025 Oct 17. PMID: 41105927
Pladienolide B purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Oct 17. [Abstract]
A375 and HCT-116 cells were treated with increasing concentrations of Pladienolide B (0-20 nM) and TG003 for 24 h and the levels of p21C and p21L were assessed with immunoblotting using 12D1 antibody; β-actin served as a loading control. OTS964 (200 nm) was used as a positive control. n = 3 biologically independent replicates.
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J Proteomics
Decoding spliceosome inhibition: Isobaric tag-based proteomic profiling of pladienolide B treated human cell lines. [Abstract]2026 Jan 20:323:105565. PMID: 41224195
Pladienolide B purchased from MedChemExpress. Usage Cited in: J Proteomics. 2026 Jan 20:323:105565. [Abstract]
A protocol for quantitative proteomics analysis of SH-SY5Y cells treated with praldinolactone B (Pla-B) based on isotope labeling was developed to determine the dose-related effects of Pla-B on the cellular proteome. Cells were treated for 24 hours with increasing concentrations of Pla-B (1 pM to 10 nM) or DMSO (as a control).
Pladienolide B purchased from MedChemExpress. Usage Cited in: J Proteomics. 2026 Jan 20:323:105565. [Abstract]
The heatmap shows the changes in protein expression under different concentrations of Pla-B (1 pM to 10 nM) treatment.
Pladienolide B purchased from MedChemExpress. Usage Cited in: J Proteomics. 2026 Jan 20:323:105565. [Abstract]
The differential protein statistics table shows the number of proteins with significant changes in Pla-B concentration from 1 pM to 10 nM.
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Solvent & Solubility
DMSO : 100 mg/mL (186.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Effenberger KA, et al. Coherence between cellular responses and in vitro splicing inhibition for the anti-tumor drug pladienolide B and its analogs. J Biol Chem. 2014 Jan 24;289(4):1938-47. [Content Brief]
[2]. Aouida M, et al. CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B. Biochim Open. 2016 Feb 24;3:72-75. [Content Brief]
[3]. Zhang Q, et al. Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells. Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1273-1280. [Content Brief]
[4]. Mizui Y, et al. Pladienolides, new substances from culture of Streptomyces platensis Mer-11107. III. In vitro and in vivo antitumor activities. J Antibiot (Tokyo). 2004 Mar;57(3):188-96. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8632 mL | 9.3162 mL | 18.6324 mL | 46.5810 mL |
| 5 mM | 0.3726 mL | 1.8632 mL | 3.7265 mL | 9.3162 mL | |
| 10 mM | 0.1863 mL | 0.9316 mL | 1.8632 mL | 4.6581 mL | |
| 15 mM | 0.1242 mL | 0.6211 mL | 1.2422 mL | 3.1054 mL | |
| 20 mM | 0.0932 mL | 0.4658 mL | 0.9316 mL | 2.3290 mL | |
| 25 mM | 0.0745 mL | 0.3726 mL | 0.7453 mL | 1.8632 mL | |
| 30 mM | 0.0621 mL | 0.3105 mL | 0.6211 mL | 1.5527 mL | |
| 40 mM | 0.0466 mL | 0.2329 mL | 0.4658 mL | 1.1645 mL | |
| 50 mM | 0.0373 mL | 0.1863 mL | 0.3726 mL | 0.9316 mL | |
| 60 mM | 0.0311 mL | 0.1553 mL | 0.3105 mL | 0.7763 mL | |
| 80 mM | 0.0233 mL | 0.1165 mL | 0.2329 mL | 0.5823 mL | |
| 100 mM | 0.0186 mL | 0.0932 mL | 0.1863 mL | 0.4658 mL |
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