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Cat. No.: HY-182428

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UAWJ248

UAWJ248 is a SARS-CoV-2 main protease (M^pro) inhibitor with an IC₅₀ of 0.012 μM. UAWJ248 inhibits activity via initial reversible binding followed by irreversible inactivation. UAWJ248 inhibits SARS-CoV-2 replication and can be used for the research of SARS-CoV-2 infection.

For research use only. We do not sell to patients.

CAS No.: 2589736-94-5
Formula: C₃₁H₄₅N₅O₇
Molecular Weight:599.72

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

UAWJ248 Chemical Structure

CAS No.: 2589736-94-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Vero C1008	EC₅₀	11.1 μM Compound: 62; UAWJ248	Antiviral activity against SARS-CoV-2 USA-WA1/2020 infected in Vero E6 cells assessed as reduction in viral replication incubated for 3 days by viral plaque assay Antiviral activity against SARS-CoV-2 USA-WA1/2020 infected in Vero E6 cells assessed as reduction in viral replication incubated for 3 days by viral plaque assay	[PMID: 37244162]

In Vitro

UAWJ248 potently inhibits tag-free native SARS-CoV-2 M^pro with an IC₅₀ of 0.012 µM and shows IC₅₀ >20 μM for PL^pro^[1].
UAWJ248 (0.01-100 µM; 48 h) inhibits SARS-CoV-2 replication in Vero E6 cells with an EC₅₀ of 20.49 µM^[1].
UAWJ248 (0.01-100 µM) inhibits SARS-CoV-2 replication in Vero E6 cells with an EC₅₀ of 11.1 µM in the plaque assay^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

Vero E6 cells
Concentration:

100 µM, 33 µM, 11 µM, 3.7 µM, 1.1 µM
Incubation Time:

48 h post-infection
Result:

Inhibited SARS-CoV-2 replication in a dose-dependent manner, with an EC₅₀ of 20.49 µM.

Chemical Information

CAS No. 2589736-94-5
Molecular Weight 599.72
Formula C₃₁H₄₅N₅O₇
SMILES

O=C(NCC1)[C@@H]1C[C@@H](C(C(NC2CC2)=O)=O)NC([C@H](CC(C)C)NC([C@H](CC(C)C)NC(OCC3=CC=CC=C3)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Sacco MD, et al. Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against Mpro and cathepsin L. bioRxiv [Preprint]. 2020 Jul 27:2020.07.27.223727. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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