O4I2
Based on 1 Customer Validation
O4I2 is a potent Oct3/4 inducer. O4I2 induces the expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1.
For research use only. We do not sell to patients.
- Purity: 99.18%
- CAS No.: 165682-93-9
- Formula: C12H11ClN2O2S
- Molecular Weight:282.75
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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USP39 |
O4I2 not only promotes Oct3/4 expression by activating its promoter site, but also stabilize the Oct3/4 protein in HEK293 cells[1].
O4I2 markedly stimulates Oct3/4 even at 5 μM after 72 hrs treatment on the translational
level[1].
O4I2 (20 μM) accumulates Oct3/4 in NCCIT occurred already 2 hrs after incubation, while the detectable Sox2 increase appears much later, implicating compound-mediated activation of Oct3/4 might be different between embryonic kidney HEK293 cells and embryonal carcinoma NCCIT cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HF (human fibroblasts) cells.
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Concentration:5-20 µM.
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Incubation Time:5 days.
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Result:Successfully detected co-expression of Oct3/4 and Sox2, as well as Nanog and Klf4.
Chemical Information
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CAS No. 165682-93-9
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Appearance Solid
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Molecular Weight 282.75
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Formula C12H11ClN2O2S
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Color Light yellow to yellow
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SMILES
O=C(C1=CSC(NC2=CC=C(Cl)C=C2)=N1)OCC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 42 mg/mL (148.54 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.84 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.5367 mL | 17.6835 mL | 35.3669 mL | 88.4173 mL |
| 5 mM | 0.7073 mL | 3.5367 mL | 7.0734 mL | 17.6835 mL | |
| 10 mM | 0.3537 mL | 1.7683 mL | 3.5367 mL | 8.8417 mL | |
| 15 mM | 0.2358 mL | 1.1789 mL | 2.3578 mL | 5.8945 mL | |
| 20 mM | 0.1768 mL | 0.8842 mL | 1.7683 mL | 4.4209 mL | |
| 25 mM | 0.1415 mL | 0.7073 mL | 1.4147 mL | 3.5367 mL | |
| 30 mM | 0.1179 mL | 0.5894 mL | 1.1789 mL | 2.9472 mL | |
| 40 mM | 0.0884 mL | 0.4421 mL | 0.8842 mL | 2.2104 mL | |
| 50 mM | 0.0707 mL | 0.3537 mL | 0.7073 mL | 1.7683 mL | |
| 60 mM | 0.0589 mL | 0.2947 mL | 0.5894 mL | 1.4736 mL | |
| 80 mM | 0.0442 mL | 0.2210 mL | 0.4421 mL | 1.1052 mL | |
| 100 mM | 0.0354 mL | 0.1768 mL | 0.3537 mL | 0.8842 mL |
O4I2 Related Classifications
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