Based on 1 Customer Validation
Wnt-C59 (C59) GMP is a GMP grade Wnt-C59 (HY-15659). GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Wnt-C59 (C59) is a highly potent and oral porcupine (PORCN) inhibitor with an IC50 of 74 pM.
For research use only. We do not sell to patients.
- CAS No.: 1243243-89-1
- Formula: C25H21N3O
- Molecular Weight:379.45
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-15659
Wnt-C59PORCN Inhibitor -
HY-13912
IWP-2Wnt Production Inhibitor -
HY-17545
LGK974PORCN Inhibitor -
HY-15659R
Wnt-C59 (Standard)PORCN Inhibitor -
HY-15825
IWP L6Porcn Inhibitor -
HY-13912G
IWP-2 (GMP)Wnt Inhibitor (GMP) -
HY-12879
IWP-4Wnt/CK1δ/ε Inhibitor -
HY-12238
IWR-1Wnt Signaling Inhibitor -
HY-18988
ETC-159PORCN Inhibitor -
HY-14428
ICG-001Apoptosis Inducer -
HY-16268
KartogeninChondroprotective Agent -
HY-12323
ISX-9Neural Stem Cell Differentiation Inducer
Biological Activity
Description
IC50 & Target
IC50: 74 pM (PORCN)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-1080 | IC50 |
74 pM
Compound: C59
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Inhibition of porcupine activity (unknown origin) expressed in human HT1080 cells assessed as suppression of Wnt3A-mediated super top flash activity by STF luciferase assay
Inhibition of porcupine activity (unknown origin) expressed in human HT1080 cells assessed as suppression of Wnt3A-mediated super top flash activity by STF luciferase assay
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[PMID: 26522946] |
In Vitro
Wnt-C59 (C59) inhibits PORCN activity in vitro at nanomolar concentrations, as assessed by inhibition of Wnt palmitoylation, Wnt interaction with the carrier protein Wntless/WLS, Wnt secretion, and Wnt activation of β-catenin reporter activity. Wnt-C59 inhibits WNT3A-mediated activation of a multimerized TCF-binding site driving luciferase with an IC50 of 74 pM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
Wnt-C59 has the potential to eradicate cancer stem cells in human tumors. Wnt-C59 inhibits stemness properties of NPC cells in a dosage-dependent manner by arresting sphere formation in both HNE1 and SUNE1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1243243-89-1
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Appearance Solid
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Molecular Weight 379.45
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Formula C25H21N3O
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Color White to off-white
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SMILES
O=C(NC1=CC=C(C2=CC=CN=C2)C=C1)CC3=CC=C(C4=CC(C)=NC=C4)C=C3
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Synonyms
C59 (GMP)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
[1]. Proffitt KD, et al. Pharmacological inhibition of the Wnt acyltransferase PORCN prevents growth of WNT-driven mammary cancer. Cancer Res. 2013 Jan 15;73(2):502-7. [Content Brief]
[2]. Cheng Y, et al. Wnt-C59 arrests stemness and suppresses growth of nasopharyngeal carcinoma in mice by inhibiting the Wnt pathway in the tumor microenvironment. Oncotarget. 2015 Jun 10;6(16):14428-39. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Wnt-C59 (GMP) Related Classifications
HY-15659G Related Classifications
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