2. Others
  3. Fluorescent Dye
  4. WST-3

WST-3 is a water-soluble tetrazolium dye. In the presence of an electron mediator, WST-3 is reduced to WST-3 formazan by NADH and displays an absorption maxima of 433 nM.

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WST-3

WST-3 Chemical Structure

CAS No. : 515111-36-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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WST-3 Related Antibodies

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Description

WST-3 is a water-soluble tetrazolium dye. In the presence of an electron mediator, WST-3 is reduced to WST-3 formazan by NADH and displays an absorption maxima of 433 nM[1].

Molecular Weight

696.34

Formula

C19H10IN6NaO10S2
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

IC1=CC=C(N2N=C(C3=CC=C(S(=O)(O[Na])=O)C=C3S(=O)([O-])=O)N=[N+]2C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (143.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4361 mL 7.1804 mL 14.3608 mL
5 mM 0.2872 mL 1.4361 mL 2.8722 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4361 mL 7.1804 mL 14.3608 mL 35.9020 mL
5 mM 0.2872 mL 1.4361 mL 2.8722 mL 7.1804 mL
10 mM 0.1436 mL 0.7180 mL 1.4361 mL 3.5902 mL
15 mM 0.0957 mL 0.4787 mL 0.9574 mL 2.3935 mL
20 mM 0.0718 mL 0.3590 mL 0.7180 mL 1.7951 mL
25 mM 0.0574 mL 0.2872 mL 0.5744 mL 1.4361 mL
30 mM 0.0479 mL 0.2393 mL 0.4787 mL 1.1967 mL
40 mM 0.0359 mL 0.1795 mL 0.3590 mL 0.8976 mL
50 mM 0.0287 mL 0.1436 mL 0.2872 mL 0.7180 mL
60 mM 0.0239 mL 0.1197 mL 0.2393 mL 0.5984 mL
80 mM 0.0180 mL 0.0898 mL 0.1795 mL 0.4488 mL
100 mM 0.0144 mL 0.0718 mL 0.1436 mL 0.3590 mL
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WST-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WST-3515111-36-1WST3WST 3Fluorescent DyeInhibitorinhibitorinhibit

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