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  3. Fluorescent Dye Biochemical Assay Reagents
  4. Xylenol blue

Xylenol Blue is a sulfonephthalein dye. It exhibits observable color changes in response to variations in pH. Xylenol Blue undergoes two distinct color transitions across two specific pH ranges: an acidic range and an alkaline range. In a neutral solution, Xylenol Blue appears yellow (at pH 8); in a strongly acidic medium, its color shifts to red (at pH 2.8); and in an alkaline medium, the yellow hue transitions to blue (at pH 9.6).

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Xylenol blue

Xylenol blue Chemical Structure

CAS No. : 125-31-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Xylenol blue Related Antibodies

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Description

Xylenol Blue is a sulfonephthalein dye. It exhibits observable color changes in response to variations in pH. Xylenol Blue undergoes two distinct color transitions across two specific pH ranges: an acidic range and an alkaline range. In a neutral solution, Xylenol Blue appears yellow (at pH 8); in a strongly acidic medium, its color shifts to red (at pH 2.8); and in an alkaline medium, the yellow hue transitions to blue (at pH 9.6)[1].

In Vitro

Xylenol Blue serves as an acid-base indicator, appearing yellow in neutral solutions (pH 8); in strongly acidic media, its color shifts to red (pH 2.8); while in alkaline media, the yellow hue transitions to blue (pH 9.6)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Xylenol blue Related Antibodies
Molecular Weight

410.48

Formula

C23H22O5S
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S1(OC(C2=C(C)C=C(O)C(C)=C2)(C3=C(C)C=C(O)C(C)=C3)C4=CC=CC=C41)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 5.83 mg/mL (14.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4362 mL 12.1809 mL 24.3617 mL
5 mM 0.4872 mL 2.4362 mL 4.8723 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4362 mL 12.1809 mL 24.3617 mL 60.9043 mL
5 mM 0.4872 mL 2.4362 mL 4.8723 mL 12.1809 mL
10 mM 0.2436 mL 1.2181 mL 2.4362 mL 6.0904 mL
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Xylenol blue Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Xylenol blue125-31-5Fluorescent DyeBiochemical Assay ReagentsInhibitorinhibitorinhibit

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