ZLD115
Based on 1 Customer Validation
ZLD115 (compound 44) is a FB23-derivated inhibitor of Fat Mass and Obesity-associated Protein (FTO). ZLD115 is an antileukemic agent, exhibiting antiproliferative activity against leukemic cell lines.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 2980653-93-6
- Formula: C27H30ClN3O4
- Molecular Weight:496.00
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEL | IC50 |
10.3 μM
Compound: 44; ZLD115
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Antiproliferative activity against HEL cells incubated for 72 hrs by CCK8 assay
Antiproliferative activity against HEL cells incubated for 72 hrs by CCK8 assay
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[PMID: 37418628] |
| KG-1 | IC50 |
4.1 μM
Compound: 44; ZLD115
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Antiproliferative activity against human KG-1 cells incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human KG-1 cells incubated for 72 hrs by CCK8 assay
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[PMID: 37418628] |
| MOLM-13 | IC50 |
1.7 μM
Compound: 44; ZLD115
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Antiproliferative activity against human MOLM-13 cells incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human MOLM-13 cells incubated for 72 hrs by CCK8 assay
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[PMID: 37418628] |
| MV4-11 | IC50 |
3.4 μM
Compound: 44; ZLD115
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Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by CCK8 assay
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[PMID: 37418628] |
| NB-4 | IC50 |
1.5 μM
Compound: 44; ZLD115
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Antiproliferative activity against human NB4 cells incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human NB4 cells incubated for 72 hrs by CCK8 assay
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[PMID: 37418628] |
| THP-1 | IC50 |
6 μM
Compound: 44; ZLD115
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Antiproliferative activity against human THP-1 cells incubated for 72 hrs by CCK8 assay
Antiproliferative activity against human THP-1 cells incubated for 72 hrs by CCK8 assay
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[PMID: 37418628] |
Chemical Information
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CAS No. 2980653-93-6
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Appearance Solid
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Molecular Weight 496.00
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Formula C27H30ClN3O4
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Color White to off-white
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SMILES
O=C(C1=C(C=CC=C1NC2=C(C=C(C=C2C3CC3)C4=C(ON=C4C)C)Cl)CN5CCCOCC5)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (201.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0161 mL | 10.0806 mL | 20.1613 mL | 50.4032 mL |
| 5 mM | 0.4032 mL | 2.0161 mL | 4.0323 mL | 10.0806 mL | |
| 10 mM | 0.2016 mL | 1.0081 mL | 2.0161 mL | 5.0403 mL | |
| 15 mM | 0.1344 mL | 0.6720 mL | 1.3441 mL | 3.3602 mL | |
| 20 mM | 0.1008 mL | 0.5040 mL | 1.0081 mL | 2.5202 mL | |
| 25 mM | 0.0806 mL | 0.4032 mL | 0.8065 mL | 2.0161 mL | |
| 30 mM | 0.0672 mL | 0.3360 mL | 0.6720 mL | 1.6801 mL | |
| 40 mM | 0.0504 mL | 0.2520 mL | 0.5040 mL | 1.2601 mL | |
| 50 mM | 0.0403 mL | 0.2016 mL | 0.4032 mL | 1.0081 mL | |
| 60 mM | 0.0336 mL | 0.1680 mL | 0.3360 mL | 0.8401 mL | |
| 80 mM | 0.0252 mL | 0.1260 mL | 0.2520 mL | 0.6300 mL | |
| 100 mM | 0.0202 mL | 0.1008 mL | 0.2016 mL | 0.5040 mL |
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