Cat. No.: HY-13777B

Zoledronic acid disodium tetrahydrate

Name: Zoledronic acid disodium tetrahydrate
Brand: MedChemExpress
SKU: HY-13777B
Availability: PreOrder

(Synonyms: Zoledronate disodium tetrahydrate; CGP 42446 disodium tetrahydrate; CGP42446A disodium tetrahydrate; ZOL 446 disodium tetrahydrate)

26 Cited Publications

Customer Review

Based on 26 publication(s) in Google Scholar

Zoledronic Acid (Zoledronate) disodium tetrahydrate is a third-generation bisphosphonate (BP), with potent anti-resorptive activity. Zoledronic Acid disodium tetrahydrate inhibits the differentiation and apoptosis of osteoclasts. Zoledronic Acid disodium tetrahydrate also has anti-cancer effects.

For research use only. We do not sell to patients.

CAS No.: 165800-07-7
Formula: C₅H₁₈N₂Na₂O₁₁P₂
Molecular Weight:390.13

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (284 KB)

Zoledronic acid disodium tetrahydrate Chemical Structure

CAS No.: 165800-07-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (26)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Zoledronic Acid disodium tetrahydrate (0.1-1 µM; 48 hours) increases receptor activator of nuclear factor kB ligand (RANKL) and sclerostin mRNA expressions in osteocyte-like MLO-Y4 cells^[2].
Zoledronic Acid disodium tetrahydrate increases the expression of osteoclastogenesis supporting factor from MLO-Y4 cells^[2].
Zoledronic Acid disodium tetrahydrate enhances the RANKL expression via IL-6/ JAK2/STAT3 pathway in MLO-Y4 cells^[2].
Zoledronic acid disodium tetrahydrate inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways^[3].
Zoledronic Acid disodium tetrahydrate (10-100 µM; 1-7 days) markedly reduces the viability of MC3T3-E1 cells and induces apoptosis in MC3T3-E1 cells^[4].
Zoledronic Acid disodium tetrahydrate (10-100 µM; 4 days) inhibits cell viability due to the induction of apoptosis^[4].
Zoledronic Acid disodium tetrahydrate exerts inhibitory effects on the differentiation and maturation of MC3T3-E1 cells at concentrations <1 µM^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

MC3T3-E1 cells
Concentration:

0.01 µM , 0.1 µM, 1 µM, 10 µM, 100 µM
Incubation Time:

1 day, 3 days, 5 days, 7 days
Result:

Reduced cells viability at 10 µM and 100 µM.

Apoptosis Analysis^[4]

Cell Line:

MC3T3-E1 cells
Concentration:

0.01 µM , 0.1 µM, 1 µM, 10 µM, 100 µM
Incubation Time:

1 days, 4 days, 7 days
Result:

Increased the number of early apoptotic cells and late apoptotic or necrotic cells at dose-dependent and time-dependent (high concentrations).

Western Blot Analysis^[4]

Cell Line:

MC3T3-E1 cells
Concentration:

0.01 µM , 0.1 µM, 1 µM, 10 µM, 100 µM
Incubation Time:

4 days
Result:

Down-regulated the protein level of inactive caspase-3 and up-regulated the protein level of active caspase-3 at the concentrations of 10 and 100 µM.

In Vivo

Zoledronic Acid disodium tetrahydrate (0.05 mg/kg; i.p.; weekly; for 3 weeks) increases bone mineral density and content^[5].
Zoledronic Acid disodium tetrahydrate (0.5-1 mg/kg; i.p.; weekly; for 3 weeks) inhibits both osteoclast and osteoblasts function and bone remodeling in vivo interfering with bone mechanical properties^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Five-week-old C57BL6 mice^[5]
Dosage:

0.05 mg/kg, 0.5 mg/kg, 1 mg/kg
Administration:

Intraperitoneal injection, weekly, for 3 weeks
Result:

Inhibited both osteoclast and osteoblasts function and bone remodeling at 0.5 mg/kg and 1 mg/kg.

Chemical Information

CAS No. 165800-07-7
Molecular Weight 390.13
Formula C₅H₁₈N₂Na₂O₁₁P₂
SMILES

OC(P(O)(O)=O)(P(O)(O)=O)CN1C=CN=C1.[H]O[H].[H]O[H].[H]O[H].[H]O[H].[Na].[Na]
Synonyms

Zoledronate disodium tetrahydrate; CGP 42446 disodium tetrahydrate; CGP42446A disodium tetrahydrate; ZOL 446 disodium tetrahydrate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (26)

All Histological Imaging/StainingIn Vivo Efficacy StudyCell Migration/Invasion AssayCell Proliferation/Viability AssayWB

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Journal Impact Factor

Natl Sci Rev

Chemical engineering of γδ T cells with cancer cell-targeting antibodies for enhanced tumor immunotherapy. [Abstract]

2025 Jun 27;12(8):nwaf256. PMID: 40842867
Nat Commun

Osteocyte mitochondria regulate angiogenesis of transcortical vessels. [Abstract]

2024 Mar 21;15(1):2529. PMID: 38514612

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Mar 21;15(1):2529. [Abstract]

Representative TRAP staining images of femur cortical bone and quantification of TRAP positive cell number from PBS or zoledronic acid (100 µg/kg, intraperitoneal injection, once per week for 2 weeks) treated 4-week-old Dmp1^Cre-DTA^ki/wt mice.

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Mar 21;15(1):2529. [Abstract]

Representative confocal images of femur cortical bone and quantitative result of TCVs from PBS or zoledronic acid (100 µg/kg, intraperitoneal injection, once per week for 2 weeks) treated 4-week-old Dmp1^Cre-DTA^ki/wt mice.
ACS Nano

A Dual-Targeted Metal-Organic Framework Based Nanoplatform for the Treatment of Rheumatoid Arthritis by Restoring the Macrophage Niche. [Abstract]

2023 Jul 25;17(14):13917-13937. PMID: 37429012
Gut Microbes

Microbial metabolite butyrate promotes anti-PD-1 antitumor efficacy by modulating T cell receptor signaling of cytotoxic CD8 T cell. [Abstract]

2023 Dec;15(2):2249143. PMID: 37635362
J Nanobiotechnology

Matrix micro/nano-topography drives oncogenic signaling and drug response in a 3D osteosarcoma model. [Abstract]

2026 Mar 6;24(1):345. PMID: 41787498
Immun Ageing

Impaired function of Vγ9Vδ2 T cells in frail elderly. [Abstract]

2026 Jan 16;23(1):7. PMID: 41546035
ACS Appl Mater Interfaces

Biomaterial-Based Lymphangiogenesis-Enhanced Therapeutic Strategy for Robustly-Integrated Medication-Related Osteonecrosis of Jaw Prevention. [Abstract]

2025 Jun 25;17(25):36315-36333. PMID: 40518597
Chem Eng J Adv

2026 Feb 16.
JCI Insight

Aerobic exercise prevents renal osteodystrophy via irisin-activated osteoblasts. [Abstract]

2025 Jan 30;10(5):e184468. PMID: 39883525

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: JCI Insight. 2025 Jan 30;10(5):e184468. [Abstract]

Serum creatinine, urea nitrogen, and uric acid levels in CKD mice or sham-operated mice (Zoledronic Acid: i.p., 20/100 μg/kg, once per day, 4 weeks).
Eur J Med Chem

Innovative medicinal chemistry strategies for enhancing drug solubility. [Abstract]

2024 Dec 5:279:116842. PMID: 39260319
Life Sci

A three-dimensional dynamic in vitro bone remodeling model reveals multicellular effects of anti-osteoporotic agents. [Abstract]

2026 Jun 15:395:124396. PMID: 41990913
Commun Biol

Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization. [Abstract]

2024 Nov 9;7(1):1476. PMID: 39521858

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Nov 9;7(1):1476. [Abstract]

Representative images and statistical graph of transwell assays from the indicated groups after treatment with ZOL at different concentrations (0, 10 and 20 μM) in UM-UC-3 cells and T24 cells for 48 h.

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Nov 9;7(1):1476. [Abstract]

An MTT assay was performed to detect changes in the proliferation of BLCA cells (UM-UC-3, T24 and 5637) after treatment with different concentrations (0, 1, 2, 5, 10, 20, 40, 60 and 80 μM) of ZOL for 48 h.

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Nov 9;7(1):1476. [Abstract]

Western blot analyses of EMT-related proteins in ZOL-treated ((0, 10 and 20 μM, 48 h) UM-UC-3, T24 and 5637 cells. GAPDH was used as the loading control.

Zoledronic acid disodium tetrahydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Nov 9;7(1):1476. [Abstract]

ZOL (100 μg/kg, intraperitoneally injection, three times a week for 4 weeks). Representative images of H&E-stained mouse lung tissue sections (n = 3)and statistical analysis of the number of metastatic nodules in H&E-stained mouse lung tissue sections.
Cell Rep Methods

RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]

2023 Oct 23;3(10):100599. PMID: 37797618
Int Immunopharmacol

Zoledronic acid enhances the efficacy of immunotherapy in non-small cell lung cancer. [Abstract]

2022 Sep:110:109030. PMID: 35978519
Med Oncol

Zoledronic acid induces ferroptosis by upregulating POR in osteosarcoma. [Abstract]

2023 Apr 10;40(5):141. PMID: 37036615
Bone

Zoledronic acid relieves steroid-induced avascular necrosis of femoral head via inhibiting FOXD3 mediated ANXA2 transcriptional activation. [Abstract]

2024 Nov:188:117222. PMID: 39102974
J Orthop Surg Res

Localized therapeutic strategy based on microRNA-21-loaded mesoporous silica nanoparticles hydrogel improves bone repair in medication-related osteonecrosis of the jaw. [Abstract]

2025 Dec 23;20(1):1079. PMID: 41437101
J Dent Sci

2026 Jan 20.
J Dent Sci

Beta-adrenergic receptor antagonist propranolol prevents bisphosphonate-related osteonecrosis of the jaw by promoting osteogenesis. [Abstract]

2025 Jan;20(1):539-552. PMID: 39873080
Int J Parasitol Drugs Drug Resist

Promising efficacy of nitrogen-containing bisphosphonates against the infection of Cryptosporidium spp. [Abstract]

2025 Aug 6:29:100607. PMID: 40782657
Appl Biochem Biotechnol

Zoledronic Acid Accelerates ER Stress-Mediated Inflammation by Increasing PDE4B Expression in Bisphosphonate-Related Osteonecrosis of the Jaw. [Abstract]

2024 Oct;196(10):7362-7374. PMID: 38523176
bioRxiv

2026 Mar 29.
SSRN

2025 Sep 23.
bioRxiv

2025 March 11.
Dis Markers

Study on the Killing Effect of γδ T Cells Activated by Rukangyin on Breast Cancer MDA-MB-231 Cells. [Abstract]

2021 Oct 15;2021:5838582. PMID: 34691287
Oxid Med Cell Longev

Zoledronic Acid Enhanced the Antitumor Effect of Cisplatin on Orthotopic Osteosarcoma by ROS-PI3K/AKT Signaling and Attenuated Osteolysis. [Abstract]

2021 Mar 30:2021:6661534. PMID: 33859780

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lianwei Wang, et al. Various pathways of zoledronic acid against osteoclasts and bone cancer metastasis: a brief review. BMC Cancer. 2020; 20: 1059. [Content Brief]

[2]. Hyung Joon Kim, et al. Zoledronate Enhances Osteocyte-Mediated Osteoclast Differentiation by IL-6/RANKL Axis. Int J Mol Sci. 2019 Mar; 20(6): 1467. [Content Brief]

[3]. Xiao-Lin Huang, et al. Zoledronic acid inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways. Int J Mol Med. 2019 Aug;44(2):582-592. [Content Brief]

[4]. XIN HUANG, et al. Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro. Mol Med Rep. 2016 Jan; 13(1): 613-622. [Content Brief]

[5]. Samantha Pozzi, et al. High-dose zoledronic acid impacts bone remodeling with effects on osteoblastic lineage and bone mechanical properties. Clin Cancer Res. 2009 Sep 15;15(18):5829-39. [Content Brief]

[6]. Shea GKH, et al. Oral Zoledronic acid bisphosphonate for the treatment of chronic low back pain with associated Modic changes: A pilot randomized controlled trial. J Orthop Res. 2022 Feb 23. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Zoledronic acid disodium tetrahydrate

26 Cited Publications

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Zoledronic acid disodium tetrahydrate

Publications Citing Use of MedChemExpress (MCE) Zoledronic acid disodium tetrahydrate