γ-Linolenic Acid methyl ester
Based on 1 Customer Validation
γ-Linolenic Acid methyl ester (Methyl GLA) is an esterified version of γ-Linolenic Acid (GLA), which is an ω-6 fatty acid, serves as melanoma cell proliferation inhibitors. γ-Linolenic Acid methyl ester inhibits ADP-induced blood platelet aggregation and induces apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.08%
- CAS No.: 16326-32-2
- Formula: C19H32O2
- Molecular Weight:292.46
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Anti-Lung Cancer Compound Library
HY-L075
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Coagulation and Anti-coagulation Compound Library
HY-L136
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
γ-Linolenic Acid methyl ester (1-4 μg/mL; 72 h) induces apoptosis in vitro A-549 lung cancer cell lines using SRB assay. However GLA is due to its action at the gene/oncogene level and by altering BCl-2 expression[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A-549 lung cancer cell line
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Concentration:1, 2, 3, and 4 μg/mL
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Incubation Time:72 hours; observed at 24 h, 48 h, and 72 h
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Result:Showed cytotoxicity potentially due to the induction of apoptosis of tumor cells by augmenting free radical generation only in the tumor cells but not normal cells.
γ-Linolenic Acid methyl ester could significantly decrease levels of plasma total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), MDA, atherosclerosis index (AI) and liver TC, MDA, and increase levels of high density lipoprotein cholesterol (HDL-C) in both normal and hyperlipidemic rats[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Hepatic pathol rats model induced by EtOH (male Sprague-Dawley rats, 250-300 g)[2]
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Dosage:90 mL, 50-60 mL
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Administration:Intraperitoneal injection; once daily; administrated 90 mL during day 1-5 and day 9, 50-60 mL during day 6-8
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Result:Decreased the hepatic triglycerides of 25.6 mg/g compared with saline (40.2 mg/g) or olive oil (42.8 mg/g) treatment.
Decreased liver index (the ratio of liver weight and body wight) in hyperlipidemic rats, but had no significant effect in normal rats.
Chemical Information
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CAS No. 16326-32-2
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Appearance Liquid (Density: 0.9019 g/cm3)
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Molecular Weight 292.46
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Formula C19H32O2
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Color Colorless to light yellow
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SMILES
CCCCC/C=C\C/C=C\C/C=C\CCCCC(OC)=O
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Synonyms
Methyl GLA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (341.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.55 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Portuguese - PT (604 KB)
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Handling Instructions (2659 KB)
References
[1]. Jubie S, et al. Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect. BMC Complement Altern Med. 2015 Aug 4;15:263. [Content Brief]
[2]. Segarnick DJ, et al. Gamma-linolenic acid inhibits the development of the ethanol-induced fatty liver. Prostaglandins Leukot Med. 1985 Mar;17(3):277-82. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4193 mL | 17.0964 mL | 34.1927 mL | 85.4818 mL |
| 5 mM | 0.6839 mL | 3.4193 mL | 6.8385 mL | 17.0964 mL | |
| 10 mM | 0.3419 mL | 1.7096 mL | 3.4193 mL | 8.5482 mL | |
| 15 mM | 0.2280 mL | 1.1398 mL | 2.2795 mL | 5.6988 mL | |
| 20 mM | 0.1710 mL | 0.8548 mL | 1.7096 mL | 4.2741 mL | |
| 25 mM | 0.1368 mL | 0.6839 mL | 1.3677 mL | 3.4193 mL | |
| 30 mM | 0.1140 mL | 0.5699 mL | 1.1398 mL | 2.8494 mL | |
| 40 mM | 0.0855 mL | 0.4274 mL | 0.8548 mL | 2.1370 mL | |
| 50 mM | 0.0684 mL | 0.3419 mL | 0.6839 mL | 1.7096 mL | |
| 60 mM | 0.0570 mL | 0.2849 mL | 0.5699 mL | 1.4247 mL | |
| 80 mM | 0.0427 mL | 0.2137 mL | 0.4274 mL | 1.0685 mL | |
| 100 mM | 0.0342 mL | 0.1710 mL | 0.3419 mL | 0.8548 mL |
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