β-Sitostenone
Based on 1 Customer Validation
β-Sitostenone (Δ4-Sitosterol-3-one; β-Rosasterol oxide) is a sterol isolated from Cleistanthus eberhardtii. β-Sitostenone inhibits tyrosinase (tyrosinase) and cyclooxygenase (COX-1 and COX-2). β-Sitostenone has potential anti-melanogenic, anticancer, and anti-inflammatory activities. β-Sitostenone can be used in research on inflammation, cancer, and cosmetics.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 1058-61-3
- Formula: C29H48O
- Molecular Weight:412.69
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
COX-1 |
COX-2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COLO 205 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human COLO205 cells after 24 hrs by MTT assay
Cytotoxicity against human COLO205 cells after 24 hrs by MTT assay
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[PMID: 30199257] |
| COLO 320 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human COLO320 cells after 24 hrs by MTT assay
Cytotoxicity against human COLO320 cells after 24 hrs by MTT assay
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[PMID: 30199257] |
| L5178Y | IC50 |
>10 μM
Compound: 3
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Antiproliferative activity against mouse L5178Y cells after 72 hrs by MTT assay
Antiproliferative activity against mouse L5178Y cells after 72 hrs by MTT assay
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[PMID: 30199257] |
| L5178Y | IC50 |
>10 μM
Compound: 3
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Antiproliferative activity against mouse L5178Y cells harboring human ABCB1 after 72 hrs by MTT assay
Antiproliferative activity against mouse L5178Y cells harboring human ABCB1 after 72 hrs by MTT assay
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[PMID: 30199257] |
| MRC5 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against human MRC5 cells after 24 hrs by MTT assay
Cytotoxicity against human MRC5 cells after 24 hrs by MTT assay
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[PMID: 30199257] |
| NIH3T3 | IC50 |
>10 μM
Compound: 3
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Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells after 24 hrs by MTT assay
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[PMID: 30199257] |
| RAW264.7 | IC50 |
15.9 μM
Compound: 114
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production
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[PMID: 31255927] |
In Vitro
β-Sitostenone (1-100 μM; 24 h) inhibits the growth of A549 non-small cell lung cancer cells[7].
β-Sitostenone (1-100 μM; 24 h) does not significantly inhibit the growth of SKOV3 ovarian cancer cells[7].
β-Sitostenone (483-485 μM) exhibits minimal inhibitory activity against both COX-1 and COX-2 enzymes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SKOV3
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Concentration:1, 100 μM
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Incubation Time:24 h
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Result:Showed cell viability of 102.78% at 100 μM.
Showed cell viability of 104.04% at 1 μM.
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Cell Line:A549
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Concentration:1, 100 μM
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Incubation Time:24 h
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Result:Showed cell viability of 71.52% at 100 μM.
Showed cell viability of 68.77% at 1 μM.
Chemical Information
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CAS No. 1058-61-3
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Appearance Solid
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Molecular Weight 412.69
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Formula C29H48O
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Color White to off-white
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CC[C@@H](CC)C(C)C)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4(C(CC3)=CC(CC4)=O)C
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Synonyms
Δ4-Sitosterol-3-one; β-Rosasterol oxide
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 2.5 mg/mL (6.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4231 mL | 12.1156 mL | 24.2313 mL | 60.5782 mL |
| 5 mM | 0.4846 mL | 2.4231 mL | 4.8463 mL | 12.1156 mL |
β-Sitostenone Related Classifications
HY-N1238 Related Classifications
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