14-Hydroxylated brassinosteroid
14-Hydroxylated brassinosteroid is a plant growth regulator. 14-Hydroxylated brassinosteroid enhances winter wheat resistance to heat stress, delays heat-induced leaf senescence, maintains leaf water content, promotes grain filling, and mitigates yield loss. 14-Hydroxylated brassinosteroid shows antioxidant activity. 14-Hydroxylated brassinosteroid can be used for the research of plant growth.
For research use only. We do not sell to patients.
- CAS No.: 457603-63-3
- Formula: C27H46O7
- Molecular Weight:482.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
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Biological Activity
Description
In Vitro
14-Hydroxylated brassinosteroid (0.05 μM) increases zeatin riboside content, reduces malondialdehyde accumulation, and enhances catalase, superoxide dismutase, and peroxidase activities in flag leaf powder of field-grown winter wheat (Triticum aestivum L. cv. JiMai 22) under heat stress[1].
14-Hydroxylated brassinosteroid (0.05 μM) delays heat-induced senescence in ex vivo flag leaves, improves grain-filling dynamics in ex vivo grain tissue, increases 1000-grain weight, and mitigates yield loss associated with heat stress in field-grown winter wheat (Triticum aestivum L. cv. JiMai 22)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 457603-63-3
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Molecular Weight 482.66
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Formula C27H46O7
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SMILES
O[C@]12[C@]3([C@@]([C@]4(C)[C@](C(=O)C3)(C[C@@H](O)[C@@H](O)C4)[H])(CC[C@]1(C)[C@@]([C@@]([C@@H](CCC(C)(C)O)O)(C)O)(CC2)[H])[H])[H]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Senescence-associated β-galactosidase staining
Senescence-associated β-galactosidase staining detects β-galactosidase activity that is histochemically visible at pH 6. 0 in senescent cells, where X-gal cleavage produces an insoluble blue precipitate observable by bright-field microscopy. This activity reflects increased lysosomal β-galactosidase/lysosomal mass rather than a senescence-essential enzyme, because GLB1 depletion or genetic lysosomal β-galactosidase deficiency can abolish SA-β-gal staining while cells still undergo senescence. SA-β-gal was originally reported in senescent but not presenescent fibroblasts and keratinocytes, absent from quiescent fibroblasts and terminally differentiated keratinocytes, and increased with donor age in human skin samples. Because SA-β-gal can also appear in some non-senescent or tissue-specific contexts, interpretation should be paired with experimental controls and, when possible, independent senescence markers.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
14-Hydroxylated brassinosteroid Related Classifications
HY-W753416 Related Classifications
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