4,4'-Dimethoxychalcone

Name: 4,4'-Dimethoxychalcone
Brand: MedChemExpress
SKU: HY-136064
Rating: 4.5 (1 reviews)

Cat. No.: HY-136064 Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4,4'-Dimethoxychalcone acts as a natural autophagy inducer with anti-ageing properties.

For research use only. We do not sell to patients.

4,4'-Dimethoxychalcone Chemical Structure

CAS No. : 2373-89-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 121	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 121	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

4,4'-Dimethoxychalcone acts as a natural autophagy inducer with anti-ageing properties^[1].

IC₅₀ & Target

Autophagy^[1]

In Vivo

The flavonoid 4,4'-Dimethoxychalcone acts as an anti-ageing compound with cardioprotective effects in mice and the potential to promote longevity across species^[1].
4,4'-Dimethoxychalcone (100 mg/kg; intraperitoneally) efficiently triggered autophagic flux in the heart and liver from wild type (WT) C57BL/6 mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6-week-old male wild type C57BL/6 or Atg4b^-/- mice^[1]
Dosage:	100 mg/kg
Administration:	Injected intraperitoneally with a single
Result:	Triggered autophagic flux in the heart and liver.

Molecular Weight

268.31

Formula

C₁₇H₁₆O₃

CAS No.

2373-89-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC=C(OC)C=C1)/C=C/C2=CC=C(OC)C=C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 250 mg/mL (931.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7270 mL	18.6352 mL	37.2703 mL
5 mM	0.7454 mL	3.7270 mL	7.4541 mL
10 mM	0.3727 mL	1.8635 mL	3.7270 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (23.29 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 6.25 mg/mL (23.29 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (270 KB) SDS (0 KB)

COA (244 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Carmona-Gutierrez D, et al. The flavonoid 4,4'-dimethoxychalcone promotes autophagy-dependent longevity across species. Nat Commun. 2019 Feb 19;10(1):651. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7270 mL	18.6352 mL	37.2703 mL	93.1758 mL
	5 mM	0.7454 mL	3.7270 mL	7.4541 mL	18.6352 mL
	10 mM	0.3727 mL	1.8635 mL	3.7270 mL	9.3176 mL
	15 mM	0.2485 mL	1.2423 mL	2.4847 mL	6.2117 mL
	20 mM	0.1864 mL	0.9318 mL	1.8635 mL	4.6588 mL
	25 mM	0.1491 mL	0.7454 mL	1.4908 mL	3.7270 mL
	30 mM	0.1242 mL	0.6212 mL	1.2423 mL	3.1059 mL
	40 mM	0.0932 mL	0.4659 mL	0.9318 mL	2.3294 mL
	50 mM	0.0745 mL	0.3727 mL	0.7454 mL	1.8635 mL
	60 mM	0.0621 mL	0.3106 mL	0.6212 mL	1.5529 mL
	80 mM	0.0466 mL	0.2329 mL	0.4659 mL	1.1647 mL
	100 mM	0.0373 mL	0.1864 mL	0.3727 mL	0.9318 mL