4,4-Dimethyl-2-cyclopenten-1-one
Based on 1 Customer Validation
4,4-Dimethyl-2-cyclopenten-1-one (4,4-Dimethylcyclopent-2-enone) is a β-unsubstituted cyclic α,β-unsaturated ketone and anticancer agent. 4,4-Dimethyl-2-cyclopenten-1-one does not activate Caspase 3, 8 or 9, nor does it induce internucleosomal DNA fragmentation. 4,4-Dimethyl-2-cyclopenten-1-one exhibits anticancer activity against oral squamous cell carcinoma and promyelocytic leukemia.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 22748-16-9
- Formula: C7H10O
- Molecular Weight:110.15
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
4,4-Dimethyl-2-cyclopenten-1-one (24 h) exhibits selective cytotoxicity against oral squamous cell carcinoma cells HSC-2, HSC-3, HSG and promyelocytic leukemia cells HL-60, but shows no obvious selectivity toward normal gingival fibroblasts HGF, dental pulp cells HPC and periodontal ligament fibroblasts HPLF. Its SI value is 4.0, and the CC50 values of tumor cell lines range from 14.0 to 152 μg/mL[2].
4,4-Dimethyl-2-cyclopenten-1-one (50 μg/mL; 24 h) exhibits cytotoxic activity against promyelocytic leukemia HL-60 cells, while co-treatment with 5 mM N-acetylcysteine (HY-B0215) significantly reduces this activity[2].
4,4-Dimethyl-2-cyclopenten-1-one (5-80 μg/mL; 6 h) does not induce internucleosomal DNA fragmentation in promyelocytic leukemia HL-60 cells even at concentrations as high as 80 μg/mL after 6 h of incubation[2].
4,4-Dimethyl-2-cyclopenten-1-one (5-80 μg/mL; 4 h) does not activate caspase-8 or caspase-9, and only exerts extremely weak or no activation on caspase-3 in promyelocytic leukemia HL-60 cells even when incubated for 4 h at concentrations up to 80 μg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human oral squamous cell carcinoma HSC-2, HSC-3, HSG cells, human promyelocytic leukemia HL-60 cells, normal human gingival fibroblast HGF, human pulp cell HPC, human periodontal ligament fibroblast HPLF cells
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Concentration:Various concentrations
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Incubation Time:24 h
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Result:Exhibited cytotoxicity against multiple tumor cell lines with CC50 values of 43.8 μg/mL (HSC-2), 152 μg/mL (HSC-3), 118 μg/mL (HSG), and 14.0 μg/mL (HL-60).
Showed lower cytotoxicity against normal cells with CC50 values of 177 μg/mL (HGF), 123 μg/mL (HPC), and 158 μg/mL (HPLF).
Demonstrated tumor-specific cytotoxicity with a selectivity index (SI = CC50 (HGF)/CC50 (HSC-2)) of 4.0.
Chemical Information
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CAS No. 22748-16-9
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Appearance Liquid (Density: 0.903 g/cm3)
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Molecular Weight 110.15
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Formula C7H10O
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Color Colorless to light yellow
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SMILES
O=C1C=CC(C)(C)C1
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Synonyms
4,4-Dimethylcyclopent-2-enone
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (907.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Korean - KR (406 KB)
- Portuguese - PT (406 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 9.0785 mL | 45.3926 mL | 90.7853 mL | 226.9632 mL |
| 5 mM | 1.8157 mL | 9.0785 mL | 18.1571 mL | 45.3926 mL | |
| 10 mM | 0.9079 mL | 4.5393 mL | 9.0785 mL | 22.6963 mL | |
| 15 mM | 0.6052 mL | 3.0262 mL | 6.0524 mL | 15.1309 mL | |
| 20 mM | 0.4539 mL | 2.2696 mL | 4.5393 mL | 11.3482 mL | |
| 25 mM | 0.3631 mL | 1.8157 mL | 3.6314 mL | 9.0785 mL | |
| 30 mM | 0.3026 mL | 1.5131 mL | 3.0262 mL | 7.5654 mL | |
| 40 mM | 0.2270 mL | 1.1348 mL | 2.2696 mL | 5.6741 mL | |
| 50 mM | 0.1816 mL | 0.9079 mL | 1.8157 mL | 4.5393 mL | |
| 60 mM | 0.1513 mL | 0.7565 mL | 1.5131 mL | 3.7827 mL | |
| 80 mM | 0.1135 mL | 0.5674 mL | 1.1348 mL | 2.8370 mL | |
| 100 mM | 0.0908 mL | 0.4539 mL | 0.9079 mL | 2.2696 mL |
4,4-Dimethyl-2-cyclopenten-1-one Related Classifications
HY-W024604 Related Classifications
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