2. Anti-infection
  3. Enterovirus
  4. 4-Chlorocinnamaldehyde

4-Chlorocinnamaldehyde  (Synonyms: trans-p-Chlorocinnamaldehyde)

Cat. No.: HY-33015A Purity: 99.83%
COA
SDS
Handling Instructions Technical Support

4-Chlorocinnamaldehyde (trans-p-Chlorocinnamaldehyde) is an antiviral agent. 4-Chlorocinnamaldehyde inhibits the replication of Coxsackievirus B3 in cardiomyocytes, but shows poor efficacy in animal experiments. 4-Chlorocinnamaldehyde can be used in studies related to viral myocarditis.

For research use only. We do not sell to patients.

4-Chlorocinnamaldehyde

4-Chlorocinnamaldehyde Chemical Structure

CAS No. : 49678-02-6

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4-Chlorocinnamaldehyde Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

4-Chlorocinnamaldehyde (trans-p-Chlorocinnamaldehyde) is an antiviral agent. 4-Chlorocinnamaldehyde inhibits the replication of Coxsackievirus B3 in cardiomyocytes, but shows poor efficacy in animal experiments. 4-Chlorocinnamaldehyde can be used in studies related to viral myocarditis[1].

In Vitro

4-Chlorocinnamaldehyde (Compound 2) (0.01-1000 μM; 72 h) shows no obvious cytotoxicity in neonatal rat cardiomyocytes, with a CC50 of 6972.47 μM after 72 h of incubation[1].
4-Chlorocinnamaldehyde (0.01-1000 μM; 72 h) weakly inhibits CVB3 replication in neonatal rat cardiomyocytes, with an IC50 of 2109.08 μM and a therapeutic index of 3.31 after 72 h of incubation[1].
4-Chlorocinnamaldehyde (10 μM; 72 h) inhibits the secretion of proinflammatory cytokines TNF-α, IL-1β and IL-6 induced by CVB3 in neonatal rat cardiomyocytes[1].
4-Chlorocinnamaldehyde (10 μM; 12-24 h) inhibits the mRNA and protein expression of proinflammatory cytokines TNF-α, IL-1β and IL-6 in neonatal rat cardiomyocytes induced by CVB3[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Chlorocinnamaldehyde Related Antibodies

ELISA Assay[1]

Cell Line: CVB3-infected neonatal rat cardiomyocytes
Concentration: 10 μM
Incubation Time: 72 h
Result: Significantly decreased TNF-α, IL-1β, and IL-6 secretion relative to CVB3-infected control cells.

Real Time qPCR[1]

Cell Line: CVB3-infected neonatal rat cardiomyocytes
Concentration: 10 μM
Incubation Time: 12 h
Result: Significantly inhibited TNF-α, IL-1β, and IL-6 mRNA expression relative to CVB3-infected control cells.

Western Blot Analysis[1]

Cell Line: CVB3-infected neonatal rat cardiomyocytes
Concentration: 10 μM
Incubation Time: 24 h
Result: Significantly reduced TNF-α, IL-1β, and IL-6 protein expression relative to CVB3-infected control cells.
In Vivo

4-Chlorocinnamaldehyde (Compound 2) (20-60 mg/kg; p.o.; daily; 6 days) does not significantly reduce myocardial pathological scores or viral titers in Coxsackievirus B3-induced viral myocarditis in male BALB/c mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (four-week-old, male, Coxsackievirus B3-induced viral myocarditis model)[1]
Dosage: 20 mg/kg; 40 mg/kg; 60 mg/kg
Administration: p.o.; daily; 6 days
Result: Showed no statistically significant differences in pathological scores or viral titers between any dose group and the model control group.
Molecular Weight

166.60

Formula

C9H7ClO
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

C(=C/C=O)\C1=CC=C(Cl)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (600.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.0024 mL 30.0120 mL 60.0240 mL
5 mM 1.2005 mL 6.0024 mL 12.0048 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 3.33 mg/mL (19.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.33 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.83%

SDS (393 KB)

COA (245 KB) HNMR (117 KB) GC (186 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.0024 mL 30.0120 mL 60.0240 mL 150.0600 mL
5 mM 1.2005 mL 6.0024 mL 12.0048 mL 30.0120 mL
10 mM 0.6002 mL 3.0012 mL 6.0024 mL 15.0060 mL
15 mM 0.4002 mL 2.0008 mL 4.0016 mL 10.0040 mL
20 mM 0.3001 mL 1.5006 mL 3.0012 mL 7.5030 mL
25 mM 0.2401 mL 1.2005 mL 2.4010 mL 6.0024 mL
30 mM 0.2001 mL 1.0004 mL 2.0008 mL 5.0020 mL
40 mM 0.1501 mL 0.7503 mL 1.5006 mL 3.7515 mL
50 mM 0.1200 mL 0.6002 mL 1.2005 mL 3.0012 mL
60 mM 0.1000 mL 0.5002 mL 1.0004 mL 2.5010 mL
80 mM 0.0750 mL 0.3752 mL 0.7503 mL 1.8758 mL
100 mM 0.0600 mL 0.3001 mL 0.6002 mL 1.5006 mL
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4-Chlorocinnamaldehyde Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Chlorocinnamaldehyde49678-02-6trans-p-ChlorocinnamaldehydeEnterovirusRhinovirusHRVHRVsHEVHEVsneonatal rat cardiomyocytesIL-1βmale BALB/c miceCoxsackievirus B3viral myocarditisTNF-αCVB3cardiomyocytesIL-6Inhibitorinhibitorinhibit

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