2. Metabolic Enzyme/Protease NF-κB
  3. Proteasome NF-κB
  4. 5-Amino-8-hydroxyquinoline

5-Amino-8-hydroxyquinoline  (Synonyms: 5A8HQ; 5AHQ)

Cat. No.: HY-W001023 Purity: 95.0%
COA
SDS
Handling Instructions Technical Support

5-Amino-8-hydroxyquinoline (5A8HQ; 5AHQ) is an orally active non-competitive 20S proteasome inhibitor. 5-Amino-8-hydroxyquinoline can inhibit NF-κB activity and induce cancer cells death and shows low cytotoxicity towards normal hematopoietic cells. 5-Amino-8-hydroxyquinoline can be used for the research of cancer, such as leukemia.

For research use only. We do not sell to patients.

5-Amino-8-hydroxyquinoline

5-Amino-8-hydroxyquinoline Chemical Structure

CAS No. : 13207-66-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
50 mg In-stock
100 mg In-stock
250 mg In-stock
500 mg   Get quote  
1 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5-Amino-8-hydroxyquinoline:

5-Amino-8-hydroxyquinoline Related Antibodies

Top Publications Citing Use of Products

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-Amino-8-hydroxyquinoline (5A8HQ; 5AHQ) is an orally active non-competitive 20S proteasome inhibitor. 5-Amino-8-hydroxyquinoline can inhibit NF-κB activity and induce cancer cells death and shows low cytotoxicity towards normal hematopoietic cells. 5-Amino-8-hydroxyquinoline can be used for the research of cancer, such as leukemia[1].

Cellular Effect
Cell Line Type Value Description References
A549 IC50
> 50 μM
Compound: 8
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
[PMID: 26276433]
HL-60 IC50
13.8 μM
Compound: 8
Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
[PMID: 26276433]
K562 IC50
0.6 μM
Compound: 5-AHQ
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human K562 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human K562 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
[PMID: 27112450]
L02 IC50
> 50 μM
Compound: 8
Cytotoxicity against human HL7702 cells after 48 hrs by MTT assay
Cytotoxicity against human HL7702 cells after 48 hrs by MTT assay
[PMID: 26276433]
MDAY-D2 IC50
0.6 μM
Compound: 5-AHQ
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human MDAY-D2 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human MDAY-D2 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
[PMID: 27112450]
NB-4 IC50
0.6 μM
Compound: 5-AHQ
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human NB4 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human NB4 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
[PMID: 27112450]
OCI-AML2 IC50
0.6 μM
Compound: 5-AHQ
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human OCI-AML2 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
Inhibition of human 20S proteasome chymotryptic-like activity using Suc-LLVY-AMC as substrate expressed in human OCI-AML2 cells assessed as free 7-amino-4-methylcoumarin release preincubated for 22 hrs followed by substrate addition by fluorescent spectrophotometric analysis
[PMID: 27112450]
SMMC-7721 IC50
> 50 μM
Compound: 8
Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay
Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay
[PMID: 26276433]
SW480 IC50
> 50 μM
Compound: 8
Cytotoxicity against human SW480 cells after 48 hrs by MTT assay
Cytotoxicity against human SW480 cells after 48 hrs by MTT assay
[PMID: 26276433]
In Vivo

5-Amino-8-hydroxyquinoline (50mg/kg; p.o.) inhibits tumor growth in the leukemia xenograft mouse models (MDAY-D2, K562, U937 cell lines)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

160.18

Formula

C9H8N2O
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

NC1=C2C=CC=NC2=C(O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (624.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.2430 mL 31.2149 mL 62.4298 mL
5 mM 1.2486 mL 6.2430 mL 12.4860 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 95.0%

SDS (393 KB)

COA (253 KB) HNMR (248 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.2430 mL 31.2149 mL 62.4298 mL 156.0744 mL
5 mM 1.2486 mL 6.2430 mL 12.4860 mL 31.2149 mL
10 mM 0.6243 mL 3.1215 mL 6.2430 mL 15.6074 mL
15 mM 0.4162 mL 2.0810 mL 4.1620 mL 10.4050 mL
20 mM 0.3121 mL 1.5607 mL 3.1215 mL 7.8037 mL
25 mM 0.2497 mL 1.2486 mL 2.4972 mL 6.2430 mL
30 mM 0.2081 mL 1.0405 mL 2.0810 mL 5.2025 mL
40 mM 0.1561 mL 0.7804 mL 1.5607 mL 3.9019 mL
50 mM 0.1249 mL 0.6243 mL 1.2486 mL 3.1215 mL
60 mM 0.1040 mL 0.5202 mL 1.0405 mL 2.6012 mL
80 mM 0.0780 mL 0.3902 mL 0.7804 mL 1.9509 mL
100 mM 0.0624 mL 0.3121 mL 0.6243 mL 1.5607 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

5-Amino-8-hydroxyquinoline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Amino-8-hydroxyquinoline13207-66-45A8HQ 5AHQProteasomeNF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
5-Amino-8-hydroxyquinoline
Cat. No.:
HY-W001023
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.