2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. 5-DACTHF

5-DACTHF  (Synonyms: 543U76; 5,11-Methenyltetrahydrohomofolate)

Cat. No.: HY-14526
Handling Instructions Technical Support

5-DACTHF (543U76) is an inhibitor of purine de novo biosynthesis. 5-DACTHF is a GAR-TFase and AICAR-TFase inhibitor (IC50: 3 and 94 μM). 5-DACTHF is a potent antitumor agent.

For research use only. We do not sell to patients.

5-DACTHF

5-DACTHF Chemical Structure

CAS No. : 118252-44-1

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

5-DACTHF Related Antibodies

Top Publications Citing Use of Products

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms
RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5-DACTHF (543U76) is an inhibitor of purine de novo biosynthesis. 5-DACTHF is a GAR-TFase and AICAR-TFase inhibitor (IC50: 3 and 94 μM). 5-DACTHF is a potent antitumor agent[1].

Cellular Effect
Cell Line Type Value Description References
A-427 IC50
43 nM
Compound: 1
Antitumor activity on A-427 lung carcinoma cell lines
Antitumor activity on A-427 lung carcinoma cell lines
[PMID: 2299624]
CCRF-CEM IC50
0.198 μM
Compound: 5-DACTHF
Cytotoxicity against human lymphoblastic leukemic CCRF-CEM cell line was evaluated as the concentration required for 50% inhibition of the growth of the control value
Cytotoxicity against human lymphoblastic leukemic CCRF-CEM cell line was evaluated as the concentration required for 50% inhibition of the growth of the control value
[PMID: 1552503]
CCRF-CEM IC50
198 nM
Compound: 2
In vitro antitumor activity was assessed from rate of cell growth of CCRF/CEM cell line
In vitro antitumor activity was assessed from rate of cell growth of CCRF/CEM cell line
[PMID: 8027993]
CCRF-CEM IC50
47 nM
Compound: 1
Antitumor activity on CCRF-CEM T-cell acute lymphoblastic leukemia cell lines
Antitumor activity on CCRF-CEM T-cell acute lymphoblastic leukemia cell lines
[PMID: 2299624]
Detroit 98 IC50
0.11 μM
Compound: 1
Evaluated in vitro for ability to inhibit the Detroit 98 cell lines
Evaluated in vitro for ability to inhibit the Detroit 98 cell lines
[PMID: 2299624]
L929 IC50
0.088 μM
Compound: 1
Evaluated in vitro for ability to inhibit the L cell lines.
Evaluated in vitro for ability to inhibit the L cell lines.
[PMID: 2299624]
MCF7 ED50
0.037 μM
Compound: 5-DACTHF
Effective dose which is required to inhibit 50% of the cell growth of MCF-7 cells in culture.
Effective dose which is required to inhibit 50% of the cell growth of MCF-7 cells in culture.
[PMID: 1995883]
MCF7 IC50
0.037 μM
Compound: 1
In vitro inhibition of growth of MCF-7 human breast adenocarcinoma using 72 hours continuous exposure
In vitro inhibition of growth of MCF-7 human breast adenocarcinoma using 72 hours continuous exposure
[PMID: 1573633]
MCF7 IC50
37 nM
Compound: 1
Antitumor activity on MCF-7 breast adenocarcinoma cell lines
Antitumor activity on MCF-7 breast adenocarcinoma cell lines
[PMID: 2299624]
MCF7 IC50
37 μM
Compound: 2
Ability to inhibit growth of MCF-7 human breast adenocarcinoma in vitro
Ability to inhibit growth of MCF-7 human breast adenocarcinoma in vitro
[PMID: 8027993]
MOLT-4 IC50
0.7 μM
Compound: 1
Hexaglutamyl homologue inhibition activity against the AICAR formyltransferase was determined against MOLT-4
Hexaglutamyl homologue inhibition activity against the AICAR formyltransferase was determined against MOLT-4
[PMID: 2299624]
MOLT-4 IC50
1.2 μM
Compound: 1
Compound was evaluated for inhibition of [3H]methotrexate uptake into MOLT-4 cells
Compound was evaluated for inhibition of [3H]methotrexate uptake into MOLT-4 cells
[PMID: 1573633]
MOLT-4 IC50
1.2 μM
Compound: 2
Ability to inhibit [3H]methotrexate transport into MOLT-4 cells in vitro
Ability to inhibit [3H]methotrexate transport into MOLT-4 cells in vitro
[PMID: 8027993]
MOLT-4 IC50
58 nM
Compound: 1
Antitumor activity on MOLT-4 T-cell acute lymphoblastic leukemia cell lines
Antitumor activity on MOLT-4 T-cell acute lymphoblastic leukemia cell lines
[PMID: 2299624]
MOLT-4 IC50
94 μM
Compound: 1
In vitro inhibitory activity against MOLT-4 T-cell leukemia cell AICAR transformylase
In vitro inhibitory activity against MOLT-4 T-cell leukemia cell AICAR transformylase
[PMID: 1573633]
MOLT-4 IC50
94 μM
Compound: 1
Inhibition activity against AICAR formyltransferase determined against MOLT-4
Inhibition activity against AICAR formyltransferase determined against MOLT-4
[PMID: 2299624]
MOLT-4 IC50
94 μM
Compound: 5-DACTHF
Inhibition of 5-aminoimidazole-4-carboxamide AICAR formyltransferase in MOLT-4 cells
Inhibition of 5-aminoimidazole-4-carboxamide AICAR formyltransferase in MOLT-4 cells
[PMID: 1995883]
SW480 IC50
50 nM
Compound: 1
Antitumor activity on SW 480 colon adenocarcinoma cell lines
Antitumor activity on SW 480 colon adenocarcinoma cell lines
[PMID: 2299624]
WiDr IC50
45 nM
Compound: 1
Antitumor activity on WiDr colon adenocarcinoma cell lines
Antitumor activity on WiDr colon adenocarcinoma cell lines
[PMID: 2299624]
WIL2 IC50
>100 μM
Compound: 5-DACTHF
Inhibition of dihydrofolate reductase (DHFR) in WIL2 cells
Inhibition of dihydrofolate reductase (DHFR) in WIL2 cells
[PMID: 1995883]
Molecular Weight

432.43

Formula

C19H24N6O6
CAS No.
SMILES

OC(CC[C@@H](C(O)=O)NC(C(C=C1)=CC=C1NCCCC2=C(NC(N)=NC2=O)N)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

5-DACTHF Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-DACTHF118252-44-1543U76 5,11-MethenyltetrahydrohomofolateDNA/RNA Synthesispurine biosynthesisantitumorMCF-7MOLT-4 cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
5-DACTHF
Cat. No.:
HY-14526
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.