Sipasterol
Based on 1 Customer Validation
Sipasterol (5α-Androstane-3β,5,6β-triol) is a neuroprotectant. Sipasterol can remarkably reverse intracellular acidification and alleviate neuronal injury through the inhibition of AMPK signaling. Sipasterol remarkably reduced the infarct volume and attenuated neurologic impairment in acute ischemic stroke models of middle cerebral artery occlusion in vivo.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 4725-51-3
- Formula: C19H32O3
- Molecular Weight:308.46
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All AMPK Isoforms
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Biological Activity
AMPK[1]
Sipasterol (TRIOL) (10 μM; for 35 min) inhibits the enhanced phosphorylation of neuronal AMPK caused by glutamate. Sipasterol also significantly attenuates the enhanced glycolysis and intracellular lactate accumulation[1].
Sipasterol (10 μM; for 35 min) significantly blocks the increase in terminal deoxynucleotidyl transferase dUTP nick-end labeling TUNEL-positive cells and the ratio of cleaved caspase-3/caspase-3 induced by glutamate[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Cerebellar Granule Neurons (CGNs).
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Concentration:10 μM
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Incubation Time:Pretreated with 20 min, followed by 200 μM Glutamate incubation for 15 min
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Result:Notably inhibited the enhanced phosphorylation of neuronal AMPK caused by Glutamate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult male SPF-level C57BL/6 mice (6-8 week old; 21-22 g) bearing permanent MCAO (pMCAO); Adult male SPF-level SD rats (7-8 week old; 270-290 g) bearing transient MCAO (tMCAO)[1]
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Dosage:1, 4, or 20 mg/kg
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Administration:Intraperitoneal injection; 30 minutes before pMCAO surgery; tMCAO was carried out by occlusion for 2 h following reperfusion injury.
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Result:Dose dependently protected C57BL/6 mice against brain injury caused by permanent focal cerebral ischemia.
Dose dependently protected SD rats against brain injury induced by transient focal cerebral ischemia.
Chemical Information
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CAS No. 4725-51-3
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Appearance Solid
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Molecular Weight 308.46
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Formula C19H32O3
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Color White to off-white
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SMILES
C[C@@]12CCC[C@@]1([H])[C@]3([H])C[C@@H](O)[C@@]4(O)C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC2
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Synonyms
5α-Androstane-3β,5,6β-triol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 125 mg/mL (405.24 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2419 mL | 16.2096 mL | 32.4191 mL | 81.0478 mL |
| 5 mM | 0.6484 mL | 3.2419 mL | 6.4838 mL | 16.2096 mL | |
| 10 mM | 0.3242 mL | 1.6210 mL | 3.2419 mL | 8.1048 mL | |
| 15 mM | 0.2161 mL | 1.0806 mL | 2.1613 mL | 5.4032 mL | |
| 20 mM | 0.1621 mL | 0.8105 mL | 1.6210 mL | 4.0524 mL | |
| 25 mM | 0.1297 mL | 0.6484 mL | 1.2968 mL | 3.2419 mL | |
| 30 mM | 0.1081 mL | 0.5403 mL | 1.0806 mL | 2.7016 mL | |
| 40 mM | 0.0810 mL | 0.4052 mL | 0.8105 mL | 2.0262 mL | |
| 50 mM | 0.0648 mL | 0.3242 mL | 0.6484 mL | 1.6210 mL | |
| 60 mM | 0.0540 mL | 0.2702 mL | 0.5403 mL | 1.3508 mL | |
| 80 mM | 0.0405 mL | 0.2026 mL | 0.4052 mL | 1.0131 mL | |
| 100 mM | 0.0324 mL | 0.1621 mL | 0.3242 mL | 0.8105 mL |
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