6-Carboxy-H2DCFDA
Based on 1 Customer Validation
6-Carboxy-H2DCFDA (6-Carboxy-2',7'-dichlorodihydrofluorescein) is a non-fluorescent, cell-permeable intracellular ROS indicator. 6-Carboxy-H2DCFDA undergoes oxidation to highly fluorescent carboxy-dichlorofluorescein (Ex/Em = 488/520 nm).
For research use only. We do not sell to patients.
- Purity : 95.22%
- CAS No.: 247044-02-6
- Formula: C25H16Cl2O9
- Molecular Weight:531.30
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
6-Carboxy-H2DCFDA (1 μM; 2 h) reliably detects intracellular ROS generation in 4-5 day cultured embryonic chick ventricular cardiomyocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 247044-02-6
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Appearance Solid
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Molecular Weight 531.30
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Formula C25H16Cl2O9
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Color White to off-white
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SMILES
O=C(C1=CC=C(C(C2C3=CC(Cl)=C(C=C3OC4=CC(OC(C)=O)=C(C=C42)Cl)OC(C)=O)=C1)C(O)=O)O
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Synonyms
6-Carboxy-2',7'-dichlorodihydrofluorescein
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 12.5 mg/mL (23.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
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Data Sheet (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8822 mL | 9.4109 mL | 18.8218 mL | 47.0544 mL |
| 5 mM | 0.3764 mL | 1.8822 mL | 3.7644 mL | 9.4109 mL | |
| 10 mM | 0.1882 mL | 0.9411 mL | 1.8822 mL | 4.7054 mL | |
| 15 mM | 0.1255 mL | 0.6274 mL | 1.2548 mL | 3.1370 mL | |
| 20 mM | 0.0941 mL | 0.4705 mL | 0.9411 mL | 2.3527 mL |
6-Carboxy-H2DCFDA Related Classifications
HY-D3376 Related Classifications
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Keywords
- 6-Carboxy-H2DCFDA
- 247044-02-6
- 6-Carboxy-2',7'-dichlorodihydrofluorescein
- Fluorescent Dye
- Reactive Oxygen Species (ROS)
- reactive oxygen species
- embryonic chick ventricular cardiomyocytes
- intracellular ROS generation
- oxidative injury
- ginsenoside Rb1
- cardiomyocytes
- carboxy-dichlorofluorescein
- Inhibitor
- inhibitor
- inhibit