6-Carboxy-H2DCFDA
Based on 1 Customer Validation
6-Carboxy-H2DCFDA (6-Carboxy-2',7'-dichlorodihydrofluorescein) is a non-fluorescent, cell-permeable intracellular ROS indicator. 6-Carboxy-H2DCFDA undergoes oxidation to highly fluorescent carboxy-dichlorofluorescein (Ex/Em = 488/520 nm).
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- Pureté: 95.22%
- CAS No.: 247044-02-6
- Formule: C25H16Cl2O9
- Masse moléculaire:531.30
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
6-Carboxy-H2DCFDA (1 μM; 2 h) reliably detects intracellular ROS generation in 4-5 day cultured embryonic chick ventricular cardiomyocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 247044-02-6
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Appearance Solid
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Masse moléculaire 531.30
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Formule C25H16Cl2O9
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Color White to off-white
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SMILES
O=C(C1=CC=C(C(C2C3=CC(Cl)=C(C=C3OC4=CC(OC(C)=O)=C(C=C42)Cl)OC(C)=O)=C1)C(O)=O)O
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Synonyms
6-Carboxy-2',7'-dichlorodihydrofluorescein
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
DMSO : 12.5 mg/mL (23.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8822 mL | 9.4109 mL | 18.8218 mL | 47.0544 mL |
| 5 mM | 0.3764 mL | 1.8822 mL | 3.7644 mL | 9.4109 mL | |
| 10 mM | 0.1882 mL | 0.9411 mL | 1.8822 mL | 4.7054 mL | |
| 15 mM | 0.1255 mL | 0.6274 mL | 1.2548 mL | 3.1370 mL | |
| 20 mM | 0.0941 mL | 0.4705 mL | 0.9411 mL | 2.3527 mL |
6-Carboxy-H2DCFDA Related Classifications
HY-D3376 Related Classifications
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Keywords
- 6-Carboxy-H2DCFDA
- 247044-02-6
- 6-Carboxy-2',7'-dichlorodihydrofluorescein
- Fluorescent Dye
- Reactive Oxygen Species (ROS)
- reactive oxygen species
- embryonic chick ventricular cardiomyocytes
- intracellular ROS generation
- oxidative injury
- ginsenoside Rb1
- cardiomyocytes
- carboxy-dichlorofluorescein
- Inhibitor
- inhibitor
- inhibit