9''-Methyl lithospermic acid
9''-Methyl lithospermic acid is a serine palmitoyltransferase (SPT) activator. 9''-Methyl lithospermic acid increases the levels of SPT1 and SPT2 in human keratinocytes. 9''-Methyl lithospermic acid maintains epidermal permeability barrier function by promoting SPT expression.
For research use only. We do not sell to patients.
- CAS No.: 1151683-53-2
- Formula: C28H24O12
- Molecular Weight:552.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
9''-Methyl lithospermic acid (Compound 2) (100 μg/mL) significantly increases SPT1 mRNA (to 139%) and SPT2 mRNA (to 139%) expression in normal human keratinocyte HaCaT cells[1].
9''-Methyl lithospermic acid (10-100 μg/mL; 9 h) dose-dependently increases SPT protein expression in normal human keratinocyte HaCaT cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HaCaT
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Concentration:10 μg/mL, 30 μg/mL, 100 μg/mL
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Incubation Time:9 h
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Result:Increased SPT protein levels to 108.9% of negative control at 10 μg/mL.
Increased SPT protein levels to 120.2% of negative control at 30 μg/mL.
Increased SPT protein levels to 123% of negative control at 100 μg/mL, showing a dose-dependent effect.
Chemical Information
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CAS No. 1151683-53-2
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Molecular Weight 552.48
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Formula C28H24O12
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SMILES
OC([C@H]1C2=C(/C=C/C(O[C@@H](C(OC)=O)CC3=CC(O)=C(C=C3)O)=O)C=CC(O)=C2O[C@@H]1C4=CC(O)=C(C=C4)O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
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Protocol for Sucrose Preference Test (SPT)
The Sucrose Preference Test is a rodent two-bottle choice assay used to estimate reward-related behavior by measuring preference for a sweet sucrose solution over water. Reduced sucrose preference is commonly interpreted as an anhedonia-like phenotype in stress-based depression models, but it can also be affected by thirst, hunger, body weight, learning, motivation, and general fluid intake.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
9''-Methyl lithospermic acid Related Classifications
HY-N20677 Related Classifications
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