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  3. BAY-524

BAY-524 is a potent Bub1 kinase inhibitor. BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC50 of 450 nM. BAY-524 can be used for the research of anti-cancer in combination with other agents.

For research use only. We do not sell to patients.

BAY-524 Chemical Structure

BAY-524 Chemical Structure

CAS No. : 1445830-39-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 425 In-stock
Solution
10 mM * 1 mL in DMSO USD 425 In-stock
Solid
1 mg USD 190 In-stock
5 mg USD 400 In-stock
10 mg USD 680 In-stock
50 mg USD 1950 In-stock
100 mg USD 2900 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BAY-524 is a potent Bub1 kinase inhibitor. BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC50 of 450 nM. BAY-524 can be used for the research of anti-cancer in combination with other agents[1].

IC50 & Target

IC50: 450 nM (human Bub1)[1]

In Vitro

BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC50 of 450 nM[1].
BAY-524 (7-10 μM or 0-30 μM; 14 h or 1 h) specifically inhibit Bub1 kinase[1].
BAY-524 (7 μM , 12 h or 7 μM, 12 μM, 3 h) affects Sgo1 and Sgo2 localization and chromatid cohesion in cells[1].
BAY-524 (7 μM, 10 μM) affects localization and activity of the CPC[1].
BAY-524 (7 μM, 2 h) exerts additive effect on centromere association of CPC[1].
BAY-524 (7 μM, 48 h) marginally affects SAC signaling[1].
BAY-524 (7 μM, 10 μM) sensitizes cells to low doses of paclitaxel[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: hTERT-RPE1 (RPE1) and HeLa cells
Concentration: 7-10 μM
Incubation Time: 14 h
Result: Drastically reduced T120 phosphorylation.

Immunofluorescence[1]

Cell Line: RPE1 and HeLa cells
Concentration: 0-30 μM
Incubation Time: 1 h
Result: Reduced histone H2A-T120 phosphorylation.
Molecular Weight

482.48

Formula

C24H24F2N6O3

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

COC1=CN=C(C2=NN(CC3=C(F)C=C(OCC)C=C3F)C(OC)=C2C)N=C1NC4=CC=NC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 166.67 mg/mL (345.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0726 mL 10.3631 mL 20.7262 mL
5 mM 0.4145 mL 2.0726 mL 4.1452 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 4.17 mg/mL (8.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 4.17 mg/mL (8.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0726 mL 10.3631 mL 20.7262 mL 51.8156 mL
5 mM 0.4145 mL 2.0726 mL 4.1452 mL 10.3631 mL
10 mM 0.2073 mL 1.0363 mL 2.0726 mL 5.1816 mL
15 mM 0.1382 mL 0.6909 mL 1.3817 mL 3.4544 mL
20 mM 0.1036 mL 0.5182 mL 1.0363 mL 2.5908 mL
25 mM 0.0829 mL 0.4145 mL 0.8290 mL 2.0726 mL
30 mM 0.0691 mL 0.3454 mL 0.6909 mL 1.7272 mL
40 mM 0.0518 mL 0.2591 mL 0.5182 mL 1.2954 mL
50 mM 0.0415 mL 0.2073 mL 0.4145 mL 1.0363 mL
60 mM 0.0345 mL 0.1727 mL 0.3454 mL 0.8636 mL
80 mM 0.0259 mL 0.1295 mL 0.2591 mL 0.6477 mL
100 mM 0.0207 mL 0.1036 mL 0.2073 mL 0.5182 mL
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BAY-524 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BAY-524
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