2. Apoptosis
  3. Apoptosis
  4. BLM-IN-1

BLM-IN-1 is an effective bloom syndrome protein (BLM) inhibitor. BLM-IN-1 has a high binding affinity with a KD valueof 1.81 μM. BLM-IN-1 has good inhibitory effect for BLM with an IC50 value of 0.95 μM. BLM-IN-1 can induce cell apoptosis. BLM-IN-1can be used for the research of DNA damage and cancer.

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BLM-IN-1 Chemical Structure

BLM-IN-1 Chemical Structure

CAS No. : 2056014-40-3

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

BLM-IN-1 Related Antibodies

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Description

BLM-IN-1 is an effective bloom syndrome protein (BLM) inhibitor. BLM-IN-1 has a high binding affinity with a KD valueof 1.81 μM. BLM-IN-1 has good inhibitory effect for BLM with an IC50 value of 0.95 μM. BLM-IN-1 can induce cell apoptosis. BLM-IN-1can be used for the research of DNA damage and cancer[1].

IC50 & Target

IC50: 0.95 μM (BLM)[1].
In Vitro

BLM-IN-1 (compound 29) has a strong BLM binding with a KD of 1.81 μM[1].
BLM-IN-1 can inhibit BLM with an IC50 of 0.95 μM[1].
BLM-IN-1 (1.0 μM) inhibits the unwinding activity of BLM but also disrupt the BLM binding to DNA[1].
BLM-IN-1 (0-1.0 μM) regulates the homologous recombination by disrupting the recruitment of BLM to DSB sites[1].
BLM-IN-1 (1.0, 2.0 μM; 48 h) induces DNA damage response and apoptosis[1].
BLM-IN-1 (0.1, 1.0, 2.0 μM; 48 h) induces proliferation arrest and synthetic interaction[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BLM-IN-1 Related Antibodies

Western Blot Analysissup>[1]

Cell Line: HCT116 cells
Concentration: 0.5, 1.0 μM
Incubation Time: 24 h
Result: Obviously upregulated the level of pATM and p-ATR in damage response.

Immunofluorescence[1]

Cell Line: HCT116 cells
Concentration: 1.0 μM
Incubation Time: 24 h
Result: Induced the accumlation of RAD51 at DSB sites.

Cell Proliferation Assay[1]

Cell Line: HCT116 cells
Concentration: 0.1, 1.0, 2.0 μM
Incubation Time: 48 h
Result: Showed a significant proliferation arrest in a dose-dependent manner in HCT116 cells.

Apoptosis Analysis[1]

Cell Line: HCT116 cells
Concentration: 1.0, 2.0 μM
Incubation Time: 48 h
Result: Increased the ratio of apoptosis cells in a concentration-dependent manner and upregulated the expression level of cleaved caspase-3, cleaved caspase-7, and cleaved PARP.
Molecular Weight

462.60

Formula

C28H35FN4O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CCN(CC)CCCNC1=CC(N=C(/C(CC2)=C/C3=CC=C(C(C)C)C=C3)N2C4=O)=C4C=C1F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (18.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1617 mL 10.8085 mL 21.6169 mL
5 mM 0.4323 mL 2.1617 mL 4.3234 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1617 mL 10.8085 mL 21.6169 mL 54.0424 mL
5 mM 0.4323 mL 2.1617 mL 4.3234 mL 10.8085 mL
10 mM 0.2162 mL 1.0808 mL 2.1617 mL 5.4042 mL
15 mM 0.1441 mL 0.7206 mL 1.4411 mL 3.6028 mL
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BLM-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BLM-IN-12056014-40-3Apoptosisbloom syndrome protein (BLM)affinityinhibitoryapoptosisDNA damagecancerInhibitorinhibitorinhibit

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