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Benfluorex (JP-992) is a hepatic nuclear factor 4 alpha (HNF4α) activator.

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Benfluorex Chemical Structure

Benfluorex Chemical Structure

CAS No. : 23602-78-0

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Benfluorex Related Antibodies

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Description

Benfluorex (JP-992) is a hepatic nuclear factor 4 alpha (HNF4α) activator.

IC50 & Target

HNF4α[1]
In Vitro

Benfluorex consistently activates insulin promoter activity as measured by an increased number of GFP-positive cells. Benfluorex increases the number of GFP-positive cells in a dose-responsive manner and increases the level of endogenous insulin mRNA. Consistent with being HNF4α activator, Benfluorex stimulates HNF4α expression. Benfluorex alters HNF4α protease sensitivity, while the inactive control compound does not[1]. Benfluorex decreases, in a concentration-dependent manner, the synthesis of acid-soluble products and ketone bodies from oleate, whereas the production of 14CO2 into citric acid cycle is markedly increased by Benfluorex. Benfluorex inhibits in a dose-dependent manner the rates of gluconeogenesis from lactate/pyruvate (10/1 nM)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Benfluorex Related Antibodies
Clinical Trial
Molecular Weight

351.36

Formula

C19H20F3NO2
CAS No.
SMILES

FC(C1=CC(CC(NCCOC(C2=CC=CC=C2)=O)C)=CC=C1)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Kinase Assay
[1]

HepG2 cells are treated with DMSO or Benfluorex at a concentration of 20 µM or 40 µM for 16 hr. Total cell protein is extracted, measured by BCA protein assay. Each sample is split into two aliquots for proteolysis without (-) or with (+) Subtilisin. Twenty ug of cell lysate is incubated with or without protease (20 ng/mL subtilisin) for 35 minutes at room temperature. Western blot is then performed with primary anti-HNF4α polyclonal antibody (1:1000 dilution) and secondary HRP conjugated anti-goat IgG (1:2000 dilution), detected with chemiluminescence ECL kit[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[2]

Hepatocytes are isolated by in situ perfusion of the liver with 0.025% collagenase. Hepatocytes (1 to 2×106 cells/mL) are incubated at 37°C in 2 mL of oxygenated (O2:CO2; 95:5) Krebs-Henseleit bicarbonate buffer (pH 7.4) for 1 h in a gyratory shaking water bath. Benfluorex is dissolved in DMSO and added (10 μL) to the incubation medium at a final concentration of 0.1 or 1 nM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Benfluorex Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benfluorex23602-78-0JP-992JP992JP 992OthersInhibitorinhibitorinhibit

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