2. Vitamin D Related/Nuclear Receptor
  3. Orphan Nuclear Receptor
  4. Benfluorex

Benfluorex (JP-992) is a hepatic nuclear factor 4 alpha (HNF4α) activator.

For research use only. We do not sell to patients.

CAS No. : 23602-78-0

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Benfluorex Related Antibodies

Top Publications Citing Use of Products

    Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451.  [Abstract]

    Relative mRNA levels of Paqr9, Ulk1, Cyp7a1 and Lipc in mouse primary hepatocytes with atorvastatin (Ator) and different concentration of Benfluorex hydrochloride (BFX) (2-50 μM).

    Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451.  [Abstract]

    Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) improved levels of fasting glucose affected by atorvastatin treatment in Paqr9-/- mice.

    Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451.  [Abstract]

    Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) improved levels of insulin and FFA affected by atorvastatin treatment in Paqr9-/- mice.

    Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451.  [Abstract]

    Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) increased hepatic AKT1 phosphorylation and lowered the PAQR9 levels raised by atorvastatin in Paqr9-/- mice.

    Benfluorex purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul 5:e2403451.  [Abstract]

    Benfluorex hydrochloride (B) (20 mg/kg; i.g.; every other day for 3 weeks) alleviated glucose tolerance and insulin tolerance of Paqr9-/- mice.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Benfluorex (JP-992) is a hepatic nuclear factor 4 alpha (HNF4α) activator.

    IC50 & Target

    HNF4α[1]
    In Vitro

    Benfluorex consistently activates insulin promoter activity as measured by an increased number of GFP-positive cells. Benfluorex increases the number of GFP-positive cells in a dose-responsive manner and increases the level of endogenous insulin mRNA. Consistent with being HNF4α activator, Benfluorex stimulates HNF4α expression. Benfluorex alters HNF4α protease sensitivity, while the inactive control compound does not[1]. Benfluorex decreases, in a concentration-dependent manner, the synthesis of acid-soluble products and ketone bodies from oleate, whereas the production of 14CO2 into citric acid cycle is markedly increased by Benfluorex. Benfluorex inhibits in a dose-dependent manner the rates of gluconeogenesis from lactate/pyruvate (10/1 nM)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Benfluorex Related Antibodies
    Molecular Weight

    351.36

    Formula

    C19H20F3NO2
    CAS No.
    SMILES

    FC(C1=CC(CC(NCCOC(C2=CC=CC=C2)=O)C)=CC=C1)(F)F
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
    Kinase Assay
    [1]

    HepG2 cells are treated with DMSO or Benfluorex at a concentration of 20 µM or 40 µM for 16 hr. Total cell protein is extracted, measured by BCA protein assay. Each sample is split into two aliquots for proteolysis without (-) or with (+) Subtilisin. Twenty ug of cell lysate is incubated with or without protease (20 ng/mL subtilisin) for 35 minutes at room temperature. Western blot is then performed with primary anti-HNF4α polyclonal antibody (1:1000 dilution) and secondary HRP conjugated anti-goat IgG (1:2000 dilution), detected with chemiluminescence ECL kit[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [2]

    Hepatocytes are isolated by in situ perfusion of the liver with 0.025% collagenase. Hepatocytes (1 to 2×106 cells/mL) are incubated at 37°C in 2 mL of oxygenated (O2:CO2; 95:5) Krebs-Henseleit bicarbonate buffer (pH 7.4) for 1 h in a gyratory shaking water bath. Benfluorex is dissolved in DMSO and added (10 μL) to the incubation medium at a final concentration of 0.1 or 1 nM[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Benfluorex Related Classifications

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Benfluorex23602-78-0JP-992JP992JP 992Orphan Nuclear ReceptorInhibitorinhibitorinhibit

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