Biotin Hydrazide

Cat. No.: HY-100215 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Biotin Hydrazide is a carbonyl-reactive biotinylation reagent, which is a carbonyl probe.

For research use only. We do not sell to patients.

Biotin Hydrazide Chemical Structure

CAS No. : 66640-86-6

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Biotin Hydrazide

•Methods Mol Biol. 2022;2509:209-231. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Biotin Hydrazide is a carbonyl-reactive biotinylation reagent, which is a carbonyl probe.

In Vitro

Biotin Hydrazide is a biotinylation reagent used to biotinylate glycoproteins and glycolipids^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

258.34

Appearance

Solid

Formula

C₁₀H₁₈N₄O₂S

CAS No.

66640-86-6

SMILES

O=C(NN)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 6.6 mg/mL (25.55 mM; Need ultrasonic and warming)

H₂O : 2 mg/mL (7.74 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8709 mL	19.3543 mL	38.7087 mL
5 mM	0.7742 mL	3.8709 mL	7.7417 mL
10 mM	0.3871 mL	1.9354 mL	3.8709 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS
Solubility: 2 mg/mL (7.74 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
2.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1 mg/mL (3.87 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1 mg/mL (3.87 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 1 mg/mL (3.87 mM); Clear solution

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (246 KB) HNMR (252 KB)

Handling Instructions (2659 KB)

References

[1]. Wu J, et al. Two-dimensional gel electrophoretic detection of protein carbonyls derivatized with biotin-hydrazide. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Apr 15;1019:128-31. [Content Brief]