1. Immunology/Inflammation
  2. Complement System

C3a 70-77 (Synonyms: Complement 3a (70-77))

Cat. No.: HY-P1505
Handling Instructions

C3a (70-77) is an octapeptide corresponding to the COOH terminus of C3a, exhibits the specificity and 1 to 2% biologic activities of C3a.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

C3a 70-77 Chemical Structure

C3a 70-77 Chemical Structure

CAS No. : 63555-63-5

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

C3a (70-77) is an octapeptide corresponding to the COOH terminus of C3a, exhibits the specificity and 1 to 2% biologic activities of C3a.

IC50 & Target

Complement system[1]

In Vitro

The direct interaction of C3a(70-77) with human mononuclear leukocytes in culture results in a concentrationdependent inhibition of the generation of LIF evoked by mitogens and by the antigen SK-SD. The extent of suppression of LIF generation by C3a(70-77) is significant at concentrations of 10-7 M or higher and exceeds 75% at 10-6 M, irrespective of the stimulus[1]. C3a (70-77), an octapeptide corresponding to the COOH terminus of human C3a, exhibits the specificity and biologic activities of C3a. Although lacking 69 residues at the NH2 terminus, it maintains 1 to 2% of the activity of natural human C3a. C3a (70-77), Ala-Ser-HisLeu-Gly-Leu-Ala-Arg, induces histamine release and degranulation of rat mast cells, promotes contraction of guinea pig ileal tissue, and enhances vascular permeability in human skin. C3a (70-77) selectively desensitizes ileal smooth muscle to C3a but not to human C5a, a related anaphylatoxin. Conversely, C3a-(70-77) is unable to contract ileal smooth muscle pretreated with natural C3a. This cross-desensitization indicates a specific interaction of C3a (70-77) with cellular C3a binding sites[2].

References
Kinase Assay
[1]

2×105 mononuelear leukocytes in 0.2 mL of M199-HPS containing 15% (vol/vol) AB-positive serum are added to each well of microtiter plates without or with 10 μg/mL of Con A, PHA, or SK-SD and incubated at 37°C in 5% CO2:95% air. C3a or C3a(70-77) is introduced into some suspensions at the same time as the stimulus or buffer. After 3 d for the mitogen-stimulated cultures and 5 d for the SK-SD-stimulated cultures, 1 μCi of [3H]thymidine is added to each well. After an additional 16 h at 37°C, the uptake of [3H]thymidine is analyzed by aspirating the contents of each well with a Mash II harvester that collected and ished the leukocytes on glass fiber filters. The radioactivity in each filter is quantified, and the results for quadruplicate chambers are expressed as mean cpm+SEM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

823.94

Formula

C₃₅H₆₁N₁₃O₁₀

CAS No.

63555-63-5

Sequence

Ala-Ser-His-Leu-Gly-Leu-Ala-Arg

Sequence Shortening

ASHLGLAR

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
C3a 70-77
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HY-P1505
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C3a 70-77

Cat. No.: HY-P1505