E-4031

Name: E-4031
Brand: MedChemExpress
SKU: HY-15551
Rating: 5.0 (7 reviews)

Cat. No.: HY-15551 Purity: 99.40%: Data Sheet
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E-4031 is a selective hERG potassium channel blocker for use in class III anti-arrhythmic studies.

For research use only. We do not sell to patients.

CAS No. : 113559-13-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of E-4031:

E-4031 (Standard) In-stock
E-4031 free base Get quote

7 Publications Citing Use of MCE E-4031

•bioRxiv. 2026 Feb 13:2026.02.11.705466.

Bio/Physico-chemical Assay

: E-4031 purchased from MedChemExpress. Usage Cited in: JCI Insight. 2025 Sep 30:e191697. [Abstract]; Western blot showing trafficked (fully glycosylated, ~155 kDa) and non-trafficked (core glycosylated, ~135 kDa) Kv11.1 protein. E4031 (10 μM; 24 h) increaseed trafficking in 2 variants.

: E-4031 purchased from MedChemExpress. Usage Cited in: J Mol Cell Biol. 2025 Jan 23:mjae051. [Abstract]; Representative whole-cell hERG currents in KCNH2-transfected HEK293T cells were recorded in the absence or presence of E-4031.

: E-4031 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2023 Apr 12;15(14):17518-17531. [Abstract]; E-4031 (50-100 nM) obviously decreased beating frequency and velocity for hiPSC-CMs on glass, AN, and AN-MR substrates. The beating rate of hiPSC-CMs decreased by 32.33 and 45.55% on glass, 33.78 and 40.6% on AN group, and 33.29 and 35.59% on AN-MR group at 50 and 100 nM conditions, respectively.

: E-4031 purchased from MedChemExpress. Usage Cited in: Biofabrication. 2022 Oct 28;15(1). [Abstract]; E-4031 (50-100 nM) notably decreased the beating frequency when acting directly on the hiPSC-CMs layer (CM-static and CM-dynamic).

: E-4031 purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2021 Nov 15:431:115731. [Abstract]; For the field potential property of hiPSC-CMs cultured for 30 days, the onset of E-4031 (100 nM) was very quick, usually within 5 min. E-4031 significantly prolonged FPDc of hiPSC-CMs.

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Description

E-4031 is a selective hERG potassium channel blocker for use in class III anti-arrhythmic studies^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	89 nM Compound: E-4031	Inhibition of human cloned ERG expressed in CHO cells assessed as inhibition of potassium channel current by automated patch clamp assay Inhibition of human cloned ERG expressed in CHO cells assessed as inhibition of potassium channel current by automated patch clamp assay	[PMID: 24805037]
CHO	IC₅₀	89 nM Compound: E-4031	Inhibition of human ERG expressed in CHO cells assessed as reduction in channel current at holding potential of 0 mV by electrophysiological method Inhibition of human ERG expressed in CHO cells assessed as reduction in channel current at holding potential of 0 mV by electrophysiological method	[PMID: 25557493]
HEK293	IC₅₀	0.02 μM Compound: 1, E-4031	Inhibition of human ERG td[wt:F656A] mutant expressed in HEK293 cells by whole cell patch clamp method Inhibition of human ERG td[wt:F656A] mutant expressed in HEK293 cells by whole cell patch clamp method	[PMID: 19260734]
HEK293	IC₅₀	0.02 μM Compound: 1, E-4031	Inhibition of wild-type human ERG expressed in HEK293 cells by whole cell patch clamp method Inhibition of wild-type human ERG expressed in HEK293 cells by whole cell patch clamp method	[PMID: 19260734]
HEK293	IC₅₀	0.25 μM Compound: 1, E-4031	Inhibition of human ERG td[wt:Y652A] mutant expressed in HEK293 cells by whole cell patch clamp method Inhibition of human ERG td[wt:Y652A] mutant expressed in HEK293 cells by whole cell patch clamp method	[PMID: 19260734]
HEK293	IC₅₀	0.69 μM Compound: 1, E-4031	Inhibition of human ERG S624A mutant expressed in HEK293 cells by whole cell patch clamp method Inhibition of human ERG S624A mutant expressed in HEK293 cells by whole cell patch clamp method	[PMID: 19260734]
HEK293	IC₅₀	2.95 μM Compound: 1, E-4031	Inhibition of human ERG F656A mutant expressed in HEK293 cells by whole cell patch clamp method Inhibition of human ERG F656A mutant expressed in HEK293 cells by whole cell patch clamp method	[PMID: 19260734]
HEK293	IC₅₀	6.78 μM Compound: 1, E-4031	Inhibition of human ERG Y652A mutant expressed in HEK293 cells by whole cell patch clamp method Inhibition of human ERG Y652A mutant expressed in HEK293 cells by whole cell patch clamp method	[PMID: 19260734]

In Vitro

E-4031 (0.1-10 μM) significantly depolarises the maximum diastolic potential (MDP) and prolongs the action charge that depolarises MDP from -58.8+0.9 to -24.5±1.8 mV at 1 μM and from -58.2±2.1 to -19.6±1.8 mV at 10 μM in single SAN cells of New Zealand albinc rabbits^[2].
E-4031 (0.1-10 μM) can block part of the outward current during the depolarisation step as well as the tail current (I_TD) during subsequent repolarization in a dose-dependent manner, and depresses I_TD by 88 % at 10 μM in single SAN cells of New Zealand albinc rabbits^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

474.44

Formula

C₂₁H₂₉Cl₂N₃O₃S

CAS No.

113559-13-0

Appearance

Solid

Color

White to off-white

SMILES

CS(=O)(NC1=CC=C(C(C2CCN(CCC3=NC(C)=CC=C3)CC2)=O)C=C1)=O.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (105.39 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1077 mL	10.5387 mL	21.0775 mL
5 mM	0.4215 mL	2.1077 mL	4.2155 mL
10 mM	0.2108 mL	1.0539 mL	2.1077 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (210.77 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (272 KB) SDS (392 KB)

COA (246 KB) RP-HPLC (304 KB)

Handling Instructions (2659 KB)

References

[1]. Daisuke Izumi, et al. Effects of bepridil versus E-4031 on transmural ventricular repolarization and inducibility of ventricular tachyarrhythmias in the dog. Pacing Clin Electrophysiol. 2010 Aug;33(8):950-9. [Content Brief]

[2]. E E Verheijck, et al. Effects of delayed rectifier current blockade by E-4031 on impulse generation in single sinoatrial nodal myocytes of the rabbit. Circ Res. 1995 Apr;76(4):607-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.1077 mL	10.5387 mL	21.0775 mL	52.6937 mL
	5 mM	0.4215 mL	2.1077 mL	4.2155 mL	10.5387 mL
	10 mM	0.2108 mL	1.0539 mL	2.1077 mL	5.2694 mL
	15 mM	0.1405 mL	0.7026 mL	1.4052 mL	3.5129 mL
	20 mM	0.1054 mL	0.5269 mL	1.0539 mL	2.6347 mL
	25 mM	0.0843 mL	0.4215 mL	0.8431 mL	2.1077 mL
	30 mM	0.0703 mL	0.3513 mL	0.7026 mL	1.7565 mL
	40 mM	0.0527 mL	0.2635 mL	0.5269 mL	1.3173 mL
	50 mM	0.0422 mL	0.2108 mL	0.4215 mL	1.0539 mL
	60 mM	0.0351 mL	0.1756 mL	0.3513 mL	0.8782 mL
	80 mM	0.0263 mL	0.1317 mL	0.2635 mL	0.6587 mL
	100 mM	0.0211 mL	0.1054 mL	0.2108 mL	0.5269 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

E-4031 Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

E-4031113559-13-0E4031E 4031Potassium ChannelKcsAhERGpotassium channelanti-arrhythmicInhibitorinhibitorinhibit

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E-4031

Customer Review

Other Forms of E-4031:

E-4031 Related Antibodies

7 Publications Citing Use of MCE E-4031

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Biological Activity

Purity & Documentation

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E-4031 Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

E-4031 Related Classifications

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