Fmoc-N-Me-Ala-OH
Based on 1 Customer Validation
Fmoc-N-Me-Ala-OH is an Fmoc-protected N-methyl-L-alanine and also a building block for solid-phase peptide synthesis. As a building block, Fmoc-N-Me-Ala-OH is used to synthesize peptidomimetic antagonists of XIAP BIR3 derived from Smac/DIABLO.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 84000-07-7
- Formula: C19H19NO4
- Molecular Weight:325.36
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Fmoc-N-Me-Ala-OH (1 μM; 1 h; 37 °C) can be incorporated into peptidomimetic compounds, and this series of peptidomimetic compounds restore caspase-9 activity inhibited by the recombinant human XIAP BIR3 domain in a dose-dependent manner (1 μM) in MDA-MB-231 cell lysates[2].
Fmoc-N-Me-Ala-OH (100 μM; 72 h; 37 °C; 5% CO2) can be incorporated into peptidomimetic compounds, which exhibit moderate cytotoxicity against MDA-MB-231 and MCF-7 breast cancer cell lines at a concentration of 100 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 84000-07-7
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Appearance Solid
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Molecular Weight 325.36
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Formula C19H19NO4
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Color White to off-white
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SMILES
C[C@H](N(C(OCC1C2=CC=CC=C2C3=CC=CC=C31)=O)C)C(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (307.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Udompholkul P, et al. Lysine Covalent Antagonists of Melanoma Inhibitors of Apoptosis Protein. J Med Chem. 2021;64(21):16147-16158. [Content Brief]
[2]. Elsawy MA, et al. Solid phase synthesis of Smac/DIABLO-derived peptides using a 'Safety-Catch' resin: identification of potent XIAP BIR3 antagonists. Bioorg Med Chem. 2013;21(17):5004-5011. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0735 mL | 15.3676 mL | 30.7352 mL | 76.8380 mL |
| 5 mM | 0.6147 mL | 3.0735 mL | 6.1470 mL | 15.3676 mL | |
| 10 mM | 0.3074 mL | 1.5368 mL | 3.0735 mL | 7.6838 mL | |
| 15 mM | 0.2049 mL | 1.0245 mL | 2.0490 mL | 5.1225 mL | |
| 20 mM | 0.1537 mL | 0.7684 mL | 1.5368 mL | 3.8419 mL | |
| 25 mM | 0.1229 mL | 0.6147 mL | 1.2294 mL | 3.0735 mL | |
| 30 mM | 0.1025 mL | 0.5123 mL | 1.0245 mL | 2.5613 mL | |
| 40 mM | 0.0768 mL | 0.3842 mL | 0.7684 mL | 1.9209 mL | |
| 50 mM | 0.0615 mL | 0.3074 mL | 0.6147 mL | 1.5368 mL | |
| 60 mM | 0.0512 mL | 0.2561 mL | 0.5123 mL | 1.2806 mL | |
| 80 mM | 0.0384 mL | 0.1921 mL | 0.3842 mL | 0.9605 mL | |
| 100 mM | 0.0307 mL | 0.1537 mL | 0.3074 mL | 0.7684 mL |
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