1. Immunology/Inflammation
  2. IRAK
  3. IRAK-1-4 Inhibitor I

IRAK-1-4 Inhibitor I  (Synonyms: IRAK-1/4 Inhibitor I)

Cat. No.: HY-13329 Purity: 99.78%
COA Handling Instructions

IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC50s of 0.2 μM and 0.3 μM, respectively.

For research use only. We do not sell to patients.

IRAK-1-4 Inhibitor I Chemical Structure

IRAK-1-4 Inhibitor I Chemical Structure

CAS No. : 509093-47-4

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Customer Review

Based on 10 publication(s) in Google Scholar

Top Publications Citing Use of Products

    IRAK-1-4 Inhibitor I purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Sep;39(36):5888-5901.  [Abstract]

    The indicated cells are treated with IRAK1/4 inhibitor for 48 h. Nucleoplasm and chromatin-bound fractions are purified and immunoblots of the indicated antibodies are analyzed. Upon IRAK1/4 inhibitor treatment, while both chromatin-bound and free nucleoplasmic MLL are proportionally increased in the parental cells, only nucleoplasmic MLL is increased in the resistant cells.

    IRAK-1-4 Inhibitor I purchased from MedChemExpress. Usage Cited in: Antibiotics. 2020 Jan 17;9(1):33.  [Abstract]

    Huh7.5.1 cells are infected with J399EM at an MOI of 0.1, and then treated with different concentrations of IRAK-1/4 inhibitor I for 72 h. The expression level of the HCV core protein is determined by western blotting. The IRAK-1/4 inhibitor I is dissolved in dimethyl sulfoxide (DMSO) and the DMSO without IRAK-1/4 inhibitor I is used as a negative control.

    View All IRAK Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC50s of 0.2 μM and 0.3 μM, respectively.

    IC50 & Target

    IC50: 0.2 μM (IRAK-4), 0.3 μM (IRAK-1)[1]

    In Vitro

    IRAK-1-4 Inhibitor I has IC50 greater than the highest concentration tested (10 μM) against a panel of 27 other kinases, including the most closely homologous (outside of the IRAK family) Lck and pp60SRC. Additionally, IRAK-1-4 Inhibitor I does not show any signs of cytotoxicity in a 72 h proliferation assay in HeLa cells (ED50>30 μM). Significant inhibition of IRAK-1 is observed with IRAK-1-4 Inhibitor I (IRAK-1 IC50=0.3 μM)[1]. IRAK-1/4 inhibitor eliminates the LPS-induced increases in Bcl10, NF-κB, and IL-8. IRAK-1/4 mediates LPS-induced IL-8 activation and functions upstream of Bcl10. The LPS-induced increase in Bcl10 declines by 73% (from 5.18±0.22 to 2.36±0.08 ng/mL), and the IL-8 response decline by 60% (from 2.64±0.31 to 1.14±0.08 ng/mL)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    395.41

    Formula

    C20H21N5O4

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=CC=CC([N+]([O-])=O)=C1)NC2=NC3=CC=CC=C3N2CCN4CCOCC4

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMF : 25 mg/mL (63.23 mM; ultrasonic and warming and heat to 60°C)

    DMSO : 5 mg/mL (12.65 mM; ultrasonic and adjust pH to 3 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : < 1 mg/mL (insoluble)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5290 mL 12.6451 mL 25.2902 mL
    5 mM 0.5058 mL 2.5290 mL 5.0580 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.88%

    References
    Cell Assay
    [2]

    NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / DMF 1 mM 2.5290 mL 12.6451 mL 25.2902 mL 63.2255 mL
    5 mM 0.5058 mL 2.5290 mL 5.0580 mL 12.6451 mL
    10 mM 0.2529 mL 1.2645 mL 2.5290 mL 6.3226 mL
    DMF 15 mM 0.1686 mL 0.8430 mL 1.6860 mL 4.2150 mL
    20 mM 0.1265 mL 0.6323 mL 1.2645 mL 3.1613 mL
    25 mM 0.1012 mL 0.5058 mL 1.0116 mL 2.5290 mL
    30 mM 0.0843 mL 0.4215 mL 0.8430 mL 2.1075 mL
    40 mM 0.0632 mL 0.3161 mL 0.6323 mL 1.5806 mL
    50 mM 0.0506 mL 0.2529 mL 0.5058 mL 1.2645 mL
    60 mM 0.0422 mL 0.2108 mL 0.4215 mL 1.0538 mL
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    IRAK-1-4 Inhibitor I Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    IRAK-1-4 Inhibitor I
    Cat. No.:
    HY-13329
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