1. Immunology/Inflammation
    Protein Tyrosine Kinase/RTK
  2. IRAK
  3. IRAK-1-4 Inhibitor I

IRAK-1-4 Inhibitor I (Synonyms: IRAK-1/4 Inhibitor I)

Cat. No.: HY-13329 Purity: 99.81%
Handling Instructions

IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC50s of 0.2 μM and 0.3 μM, respectively.

For research use only. We do not sell to patients.

IRAK-1-4 Inhibitor I Chemical Structure

IRAK-1-4 Inhibitor I Chemical Structure

CAS No. : 509093-47-4

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10 mM * 1 mL in DMSO USD 118 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

IRAK-1-4 Inhibitor I is an inhibitor of interleukin-1 receptor-associated kinase 1/4 (IRAK 1/4) with IC50s of 0.2 μM and 0.3 μM, respectively.

IC50 & Target

IC50: 0.2 μM (IRAK-4), 0.3 μM (IRAK-1)[1]

In Vitro

IRAK-1-4 Inhibitor I has IC50 greater than the highest concentration tested (10 μM) against a panel of 27 other kinases, including the most closely homologous (outside of the IRAK family) Lck and pp60SRC. Additionally, IRAK-1-4 Inhibitor I does not show any signs of cytotoxicity in a 72 h proliferation assay in HeLa cells (ED50>30 μM). Significant inhibition of IRAK-1 is observed with IRAK-1-4 Inhibitor I (IRAK-1 IC50=0.3 μM)[1]. IRAK-1/4 inhibitor eliminates the LPS-induced increases in Bcl10, NF-κB, and IL-8. IRAK-1/4 mediates LPS-induced IL-8 activation and functions upstream of Bcl10. The LPS-induced increase in Bcl10 declines by 73% (from 5.18±0.22 to 2.36±0.08 ng/mL), and the IL-8 response decline by 60% (from 2.64±0.31 to 1.14±0.08 ng/mL)[2].

Molecular Weight

395.41

Formula

C₂₀H₂₁N₅O₄

CAS No.

509093-47-4

SMILES

O=C(C1=CC=CC([N+]([O-])=O)=C1)NC2=NC3=CC=CC=C3N2CCN4CCOCC4

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 14.29 mg/mL (36.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5290 mL 12.6451 mL 25.2902 mL
5 mM 0.5058 mL 2.5290 mL 5.0580 mL
10 mM 0.2529 mL 1.2645 mL 2.5290 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 1.43 mg/mL (3.62 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.81%

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IRAK-1-4 Inhibitor I
Cat. No.:
HY-13329
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