1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Indole-3-butyric acid

Indole-3-butyric acid  (Synonyms: Indolebutyric acid)

Cat. No.: HY-N0186 Purity: 99.94%
COA Handling Instructions

Indole-3-butyric acid (3-indolebutyric acid) is a plant growth auxin and a good rooting agent. It can promote herbs and woody ornamental plant rooting and used for improving fruit rate. Indole 3-butyric acid is an auxin precursor, and is converted to indole 3-acetic acid (IAA) in a peroxisomal β-oxidation process.

For research use only. We do not sell to patients.

Indole-3-butyric acid Chemical Structure

Indole-3-butyric acid Chemical Structure

CAS No. : 133-32-4

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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

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Description

Indole-3-butyric acid (3-indolebutyric acid) is a plant growth auxin and a good rooting agent. It can promote herbs and woody ornamental plant rooting and used for improving fruit rate. Indole 3-butyric acid is an auxin precursor, and is converted to indole 3-acetic acid (IAA) in a peroxisomal β-oxidation process[1].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Indole-3-butyric acid (10 μM) induces adventitious root (AR) formation in the thin cell layers (TCLs)[2].
Indole-3-butyric acid (1 μM) induces lateral root formation by the promotion of NO production in Arabidopsis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

203.24

Formula

C12H13NO2

CAS No.
Appearance

Solid

Color

Off-white to pink

SMILES

O=C(O)CCCC1=CNC2=C1C=CC=C2

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 35 mg/mL (172.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9203 mL 24.6015 mL 49.2029 mL
5 mM 0.9841 mL 4.9203 mL 9.8406 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (12.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.94%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.9203 mL 24.6015 mL 49.2029 mL 123.0073 mL
5 mM 0.9841 mL 4.9203 mL 9.8406 mL 24.6015 mL
10 mM 0.4920 mL 2.4601 mL 4.9203 mL 12.3007 mL
15 mM 0.3280 mL 1.6401 mL 3.2802 mL 8.2005 mL
20 mM 0.2460 mL 1.2301 mL 2.4601 mL 6.1504 mL
25 mM 0.1968 mL 0.9841 mL 1.9681 mL 4.9203 mL
30 mM 0.1640 mL 0.8200 mL 1.6401 mL 4.1002 mL
40 mM 0.1230 mL 0.6150 mL 1.2301 mL 3.0752 mL
50 mM 0.0984 mL 0.4920 mL 0.9841 mL 2.4601 mL
60 mM 0.0820 mL 0.4100 mL 0.8200 mL 2.0501 mL
80 mM 0.0615 mL 0.3075 mL 0.6150 mL 1.5376 mL
100 mM 0.0492 mL 0.2460 mL 0.4920 mL 1.2301 mL
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Indole-3-butyric acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Indole-3-butyric acid
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