Cat. No.: HY-W012382

N-Acetyl-L-tyrosine

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N-Acetyl-L-tyrosine is an orally active endogenous mitochondrial stress response regulator that can permeate the cell membrane by passive diffusion. N-Acetyl-L-tyrosine induces low-level reactive oxygen species (ROS) generation by transiently perturbing mitochondrial membrane potential, triggering reverse signaling to activate FoxO and Keap1 pathways. As a result, N-Acetyl-L-tyrosine enhances the expression of antioxidant enzyme genes, exerting anti-stress and cytoprotective effects. N-Acetyl-L-tyrosine can improve heat stress tolerance, inhibit tumor growth, and regulate energy metabolism. N-Acetyl-L-tyrosine can be used in the research of aging, metabolic diseases (such as diabetes), and cancer.

For research use only. We do not sell to patients.

Purity: 99.48%
CAS No.: 537-55-3
Formula: C₁₁H₁₃NO₄
Molecular Weight:223.23

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

N-Acetyl-L-tyrosine Chemical Structure

CAS No.: 537-55-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vivo

N-Acetyl-L-tyrosine (0.4%, added to the diet; 7 days) is the main source of tyrosine for growing rats. Rats not supplemented with N-Acetyl-L-tyrosine shows signs of malnutrition, while rats in the treated group shows increased nitrogen retention and thymus weight^[1].
N-Acetyl-L-tyrosine (10 nmol/larva; injection; single dose) significantly increases the survival rate of larvae after heat stress (44°C) in the heat stress model of armyworms (Mythimna separata), inducing heat tolerance in armyworms^[2].
N-Acetyl-L-tyrosine (5 mg/mL, added to drinking water; 7 days) inhibits the growth of HCT116 tumor cells and downregulates tumor volume in a nude mouse colon cancer transplantation model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/cSlc-nu/nu nude mice (female, 6 weeks old, ~18 g) with HCT116 colon cancer xenograft model^[1]

Dosage:

5 mg/mL N-Acetyl-L-tyrosine in drinking water (sterile water); the average amount of N-Acetyl-L-tyrosine consumed by each mouse was about 8.8 mg/day (0.4-0.5 g/kg weight/day).

Administration:

Free access to N-Acetyl-L-tyrosine-containing water for 7 days before tumor cell transplantation (day 0), continued throughout the 17-day observation period.

Result:

Significantly decreased tumor volume at day 17.
Slightly reduced body weight without severe toxicity.
Increased FoxO and Keap1 expression in tumor tissues, and reduced Nrf2 signaling due to the regulation of antioxidant and tumor-suppressive pathways.

Chemical Information

CAS No. 537-55-3
Appearance Solid
Molecular Weight 223.23
Formula C₁₁H₁₃NO₄
Color White to off-white
SMILES

OC1=CC=C(C=C1)C[C@@H](C(O)=O)NC(C)=O
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (447.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (447.97 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.4797 mL

22.3984 mL

44.7968 mL

5 mM

0.8959 mL

4.4797 mL

8.9594 mL

10 mM

0.4480 mL

2.2398 mL

4.4797 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (223.98 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bjelton, Lilian, et al. N-acetyl-L-tyrosine as a source of tyrosine in intravenous nutrition an experimental study in the rat. Nutrition Research 9.5 (1989): 555-564.

[2]. Matsumura T, et al. N-acetyl-l-tyrosine is an intrinsic triggering factor of mitohormesis in stressed animals. EMBO Rep. 2020 May 6;21(5):e49211. [Content Brief]

[3]. Kumar R, et al. Synthesis and evaluation of N-acetyl-L-tyrosine based compounds as PPARalpha selective activators. Eur J Med Chem. 2007 Apr;42(4):503-10. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	4.4797 mL	22.3984 mL	44.7968 mL	111.9921 mL
	5 mM	0.8959 mL	4.4797 mL	8.9594 mL	22.3984 mL
	10 mM	0.4480 mL	2.2398 mL	4.4797 mL	11.1992 mL
	15 mM	0.2986 mL	1.4932 mL	2.9865 mL	7.4661 mL
	20 mM	0.2240 mL	1.1199 mL	2.2398 mL	5.5996 mL
	25 mM	0.1792 mL	0.8959 mL	1.7919 mL	4.4797 mL
	30 mM	0.1493 mL	0.7466 mL	1.4932 mL	3.7331 mL
	40 mM	0.1120 mL	0.5600 mL	1.1199 mL	2.7998 mL
	50 mM	0.0896 mL	0.4480 mL	0.8959 mL	2.2398 mL
	60 mM	0.0747 mL	0.3733 mL	0.7466 mL	1.8665 mL
	80 mM	0.0560 mL	0.2800 mL	0.5600 mL	1.3999 mL
	100 mM	0.0448 mL	0.2240 mL	0.4480 mL	1.1199 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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N-Acetyl-L-tyrosine

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All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

N-Acetyl-L-tyrosine Related Classifications

HY-W012382 Related Classifications