N6022
Based on 14 publication(s) in Google Scholar
N6022 is a potent, selective, reversible, and efficacious S-Nitrosoglutathione reductase(GSNOR) inhibitor with IC50 of 8 nM.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 1208315-24-5
- Formula: C24H22N4O3
- Molecular Weight:414.46
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) N6022
More- Circ Res. 2025 Oct 10;137(9):1185-1206. [Abstract]
- Adv Sci (Weinh). 2024 Jul;11(26):e2403894. [Abstract]
- Food Chem. 2022 Oct 30:392:133299. [Abstract]
- Cell Death Dis. 2019 May; 10(5): 329. [Abstract]
- Free Radic Biol Med. 2017 Jul:108:445-451. [Abstract]
- Bone. 2024 Apr:181:117036. [Abstract]
- Mol Pharmacol. 2016 Oct;90(4):418-26. [Abstract]
- Pediatr Pulm. 2019 Dec;54(12):1989-1996. [Abstract]
- Cytotechnology. 2025 Jun;77(3):102. [Abstract]
- Brain Dev. 2025 Jul 1;47(4):104388. [Abstract]
- Patent. US20200206195A1
- Patent. US20170209419A1.
- University of Windsor. 2017 Apr.
- Case Western Reserve University. 2016 Aug.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
Biological Activity
IC50: 8 nM (GSNOR)[2]
N6022 shows concentration-dependent binding to rat plasma proteins. N6022 has more effect on ATP at lower drug concentrations (20 μM) than on GSH[1]. N6022 binds in the GSNO substrate binding pocket like a competitive inhibitor with an IC50 of 8 nM and a Ki of 2.5 nM. N6022 is uncompetitive with cofactors NAD+ and NADH[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 1208315-24-5
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Appearance Solid
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Molecular Weight 414.46
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Formula C24H22N4O3
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Color Off-white to yellow
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SMILES
OC(CCC1=CC=C(N1C2=CC=C(C(N)=O)C=C2C)C(C=C3)=CC=C3N4C=CN=C4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (14)
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Journal Impact Factor
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Most Recent
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Circ Res
Dysregulated Protein s-Nitrosylation Promotes Nitrosative Stress and Disease Progression in Heart Failure With Preserved Ejection Fraction. [Abstract]2025 Oct 10;137(9):1185-1206. PMID: 40959880 -
Adv Sci (Weinh)
De-nitrosylation Coordinates Appressorium Function for Infection of the Rice Blast Fungus. [Abstract]2024 Jul;11(26):e2403894. PMID: 38704696 -
Food Chem
GABA keeps nitric oxide in balance by regulating GSNOR to enhance disease resistance of harvested tomato against Botrytis cinerea. [Abstract]2022 Oct 30:392:133299. PMID: 35640428 -
Cell Death Dis
S-nitrosylation of the Peroxiredoxin-2 promotes S-nitrosoglutathione-mediated lung cancer cells apoptosis via AMPK-SIRT1 pathway. [Abstract]2019 May; 10(5): 329. PMID: 30988280
N6022 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 May; 10(5): 329. [Abstract]
The synergistic effects of GSNO (0.3 mM) with N6022 (0.5 μM) on cell viability analysis of A549 (left) and NCI-H1299 cells (right).
N6022 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 May; 10(5): 329. [Abstract]
Cleavage of Caspase-3 expression in A549 cells treated with GSNO or/and N6022 (0.5 μM) for 24 h was probed by WB.
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Free Radic Biol Med
O-Aminobenzoyl-S-nitrosoglutathione: A fluorogenic, cell permeable, pseudo-substrate for S-nitrosoglutathione reductase. [Abstract]2017 Jul:108:445-451. PMID: 28419866 -
Bone
S-nitrosoglutathione reductase-dependent p65 denitrosation promotes osteoclastogenesis by facilitating recruitment of p65 to NFATc1 promoter. [Abstract]2024 Apr:181:117036. PMID: 38311303 -
Mol Pharmacol
S-Nitrosoglutathione Attenuates Airway Hyperresponsiveness in Murine Bronchopulmonary Dysplasia. [Abstract]2016 Oct;90(4):418-26. PMID: 27484068 -
Pediatr Pulm
Tracheomalacia in bronchopulmonary dysplasia: Trachealis hyper-relaxant responses to S-nitrosoglutathione in a hyperoxic murine model. [Abstract]2019 Dec;54(12):1989-1996. PMID: 31486289 -
Cytotechnology
LncRNA OIP5-AS1 promotes immune evasion in abdominal aortic aneurysm cells by recruiting MDSCs and inhibiting CD8 + T cells through STK24 upregulation. [Abstract]2025 Jun;77(3):102. PMID: 40384846 -
Brain Dev
L-NAME improves the morphology and necrosis of skeletal muscle by activating PINK1-PARKIN mediated mitophagy in mdx mice. [Abstract]2025 Jul 1;47(4):104388. PMID: 40602311 -
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Solvent & Solubility
DMSO : 25 mg/mL (60.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
N6022 is tested using a rat hepatoma (H4IIE) cell line whereby cells are seeded into 96-well plates and cultured in medium containing 20% bovine serum. Following an equilibration period of 48 h, the cells are treated with N6022 (5% DMSO vehicle) at concentrations of 0, 1, 5, 10, 20, 50, 100, and 300 μM for 24 h at 37°C in 5% CO2. Camptothecin and rotenone are included as positive controls. The cell supernatant or the cells themselves are harvested for biochemical analysis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sun X, et al. Structure-activity relationships of pyrrole based S-nitrosoglutathione reductase inhibitors: pyrrole regioisomers and propionic acid replacement. Bioorg Med Chem Lett. 2011 Jun 15;21(12):3671-5. [Content Brief]
[2]. Colagiovanni DB, et al. A nonclinical safety and pharmacokinetic evaluation of N6022: a first-in-class S-nitrosoglutathione reductase inhibitor for the treatment of asthma. Regul Toxicol Pharmacol. 2012 Feb;62(1):115-24. [Content Brief]
[3]. Green LS, et al. Mechanism of inhibition for N6022, a first-in-class drug targeting S-nitrosoglutathione reductase. Biochemistry. 2012 Mar 13;51(10):2157-68. [Content Brief]
[4]. Thomas M. Raffay, et al. Methods of treating respiratory disorders. Patent. US 20170209419 A1.
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4128 mL | 12.0639 mL | 24.1278 mL | 60.3195 mL |
| 5 mM | 0.4826 mL | 2.4128 mL | 4.8256 mL | 12.0639 mL | |
| 10 mM | 0.2413 mL | 1.2064 mL | 2.4128 mL | 6.0319 mL | |
| 15 mM | 0.1609 mL | 0.8043 mL | 1.6085 mL | 4.0213 mL | |
| 20 mM | 0.1206 mL | 0.6032 mL | 1.2064 mL | 3.0160 mL | |
| 25 mM | 0.0965 mL | 0.4826 mL | 0.9651 mL | 2.4128 mL | |
| 30 mM | 0.0804 mL | 0.4021 mL | 0.8043 mL | 2.0106 mL | |
| 40 mM | 0.0603 mL | 0.3016 mL | 0.6032 mL | 1.5080 mL | |
| 50 mM | 0.0483 mL | 0.2413 mL | 0.4826 mL | 1.2064 mL | |
| 60 mM | 0.0402 mL | 0.2011 mL | 0.4021 mL | 1.0053 mL |
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