Piperazine Erastin

Name: Piperazine Erastin
Brand: MedChemExpress
SKU: HY-100887
Rating: 5.0 (9 reviews)

Cat. No.: HY-100887 Purity: 98.25%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Piperazine erastin is an analog of erastin which induces an iron-dependent form of non-apoptotic cell death, termed ferroptosis. Piperazine erastin can be used in cancer research.

For research use only. We do not sell to patients.

Piperazine Erastin Chemical Structure

CAS No. : 1538593-71-3

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 307	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 307	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 108	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 216	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1180	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 9 publication(s) in Google Scholar

9 Publications Citing Use of MCE Piperazine Erastin

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Biological Activity
Protocol
Purity & Documentation
Customer Review

Description

Piperazine erastin is an analog of erastin which induces an iron-dependent form of non-apoptotic cell death, termed ferroptosis. Piperazine erastin can be used in cancer research^[1].

In Vitro

Erastin is a ferroptosis activator. It triggers a unique iron-dependent form of non-apoptotic cell death that is termed as ferroptosis. Piperazine erastin is a more effective analog of erastin which is more water-soluble (0.086 mM for erastin versus 1.4 mM for piperazine erastin) and more metabolically stable. Piperazine erastin is affected similarly by cell death modulators as erastin and displays a distinct pattern from other non-FIN lethal compounds^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In the xenograft mouse model, a significant delay in tumor growth is observed in the piperazine erastin-treated group compared to the vehicle-treated group. Ptgs2 is upregulated in mouse liver with 10 or 60 mg/kg piperazine erastin administration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

645.19

Formula

C₃₅H₄₁ClN₆O₄

CAS No.

1538593-71-3

Appearance

Solid

SMILES

O=C1N(C2=CC(CN3CCNCC3)=CC=C2OC(C)C)C(CN4CCN(C(COC5=CC=C(Cl)C=C5)=O)CC4)=NC6=C1C=CC=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (51.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5499 mL	7.7497 mL	15.4993 mL
5 mM	0.3100 mL	1.5499 mL	3.0999 mL
10 mM	0.1550 mL	0.7750 mL	1.5499 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.25%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (134 KB) HNMR (335 KB) RP-HPLC (215 KB)

Handling Instructions (2659 KB)

Animal Administration
^[1]

Mice: Athymic nude mice are injected with four million HT-1080 cells s.c. The next day, 400 μL of vehicle (0.625% DMSO/99.375% HBSS [pH 2]) or 40 mg/kg piperazine erastin is delivered to the s.c. site where cancer cells are injected. Two days later, the s.c. injection is repeated. Three days later, 300 μL of vehicle or 30 mg/kg piperazine erastin is administered to the mice through tail vein. Tail vein injection is repeated three more times, once every other day before the final tumor size is measured in both groups^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5499 mL	7.7497 mL	15.4993 mL	38.7483 mL
	5 mM	0.3100 mL	1.5499 mL	3.0999 mL	7.7497 mL
	10 mM	0.1550 mL	0.7750 mL	1.5499 mL	3.8748 mL
	15 mM	0.1033 mL	0.5166 mL	1.0333 mL	2.5832 mL
	20 mM	0.0775 mL	0.3875 mL	0.7750 mL	1.9374 mL
	25 mM	0.0620 mL	0.3100 mL	0.6200 mL	1.5499 mL
	30 mM	0.0517 mL	0.2583 mL	0.5166 mL	1.2916 mL
	40 mM	0.0387 mL	0.1937 mL	0.3875 mL	0.9687 mL
	50 mM	0.0310 mL	0.1550 mL	0.3100 mL	0.7750 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Piperazine Erastin1538593-71-3Inhibitorinhibitorinhibit

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Piperazine Erastin

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