1. Cell Cycle/DNA Damage
  2. RAD51
  3. RI-2

RI-2 is a reversible RAD51 inhibitor, with an IC50 of 44.17 μM, and specifically inhibits homologous recombination repair in human cells.

For research use only. We do not sell to patients.

RI-2 Chemical Structure

RI-2 Chemical Structure

CAS No. : 1417162-36-7

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 220 In-stock
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10 mM * 1 mL in DMSO USD 220 In-stock
Solid
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10 mg USD 360 In-stock
50 mg USD 900 In-stock
100 mg USD 1650 In-stock
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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE RI-2

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

RI-2 is a reversible RAD51 inhibitor, with an IC50 of 44.17 μM, and specifically inhibits homologous recombination repair in human cells.

IC50 & Target

IC50: 44.17 μM (RAD51)[1]

In Vitro

RI-2 (7a) is a reversible RAD51 inhibitor, with an IC50 of 44.17 μM. RI-2 specifically inhibits homologous recombination repair in human cells. RI-2 (150 μM) induces a significant sensitization of cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

433.28

Formula

C21H18Cl2N2O4

CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(C(C1=CC=C(OC)C=C1)=C2N3CCOCC3)N(C4=CC=C(Cl)C(Cl)=C4)C2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 130 mg/mL (300.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3080 mL 11.5399 mL 23.0798 mL
5 mM 0.4616 mL 2.3080 mL 4.6160 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.64%

References
Cell Assay
[1]

HEK293 cells are plated into 96-well tissue culture plates at a density of 300 cells per well in the presence or absence of 50 nM mitomycin C (MMC) for 24 hours at 37°C, 5% CO2. Media is subsequently replaced with fresh media containing 0.5% DMSO plus RI-2 for an additional 24 hours. RI-2 is then removed, and cultures are allowed to grow to a 50-70% confluence. Average survival from at least three replicates is measured using CellGlo reagentor. RI-2 is deemed successful in sensitizing cells to MMC if they generate significantly greater toxicity in the presence of MMC relative to the absence of MMC. Specifically, sensitization is scored as a “+” when non-overlapping standard errors are observed for at least two pairs of compound doses[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3080 mL 11.5399 mL 23.0798 mL 57.6994 mL
5 mM 0.4616 mL 2.3080 mL 4.6160 mL 11.5399 mL
10 mM 0.2308 mL 1.1540 mL 2.3080 mL 5.7699 mL
15 mM 0.1539 mL 0.7693 mL 1.5387 mL 3.8466 mL
20 mM 0.1154 mL 0.5770 mL 1.1540 mL 2.8850 mL
25 mM 0.0923 mL 0.4616 mL 0.9232 mL 2.3080 mL
30 mM 0.0769 mL 0.3847 mL 0.7693 mL 1.9233 mL
40 mM 0.0577 mL 0.2885 mL 0.5770 mL 1.4425 mL
50 mM 0.0462 mL 0.2308 mL 0.4616 mL 1.1540 mL
60 mM 0.0385 mL 0.1923 mL 0.3847 mL 0.9617 mL
80 mM 0.0288 mL 0.1442 mL 0.2885 mL 0.7212 mL
100 mM 0.0231 mL 0.1154 mL 0.2308 mL 0.5770 mL
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RI-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RI-2
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HY-16904
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