SV40 large T antigen NLS

Name: SV40 large T antigen NLS
Brand: MedChemExpress
SKU: HY-P0310
Rating: 4.5 (1 reviews)

Cat. No.: HY-P0310 Purity: 99.34%: Data Sheet
COA

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SV40 large T antigen NLS is from Large T antigen residue 47 to 55, enables protein import into cell nucleus.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

SV40 large T antigen NLS Chemical Structure

CAS No. : 163815-24-5

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
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Description

SV40 large T antigen NLS is from Large T antigen residue 47 to 55, enables protein import into cell nucleus.

In Vitro

SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS)^[1]. T-ag NLS can enhance nuclear import, despite the fact that PK-C has been shown to inhibit nuclear import of proteins such as lamin B2^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1401.63

Formula

C₅₈H₁₀₄N₂₀O₁₈S

CAS No.

163815-24-5

Appearance

Solid

Color

White to off-white

Sequence

Cys-Gly-Gly-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Val-Glu-Asp

Sequence Shortening

CGGGPKKKRKVED

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (71.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7135 mL	3.5673 mL	7.1346 mL
5 mM	0.1427 mL	0.7135 mL	1.4269 mL
10 mM	0.0713 mL	0.3567 mL	0.7135 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.34%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (255 KB) RP-HPLC (112 KB) MS (187 KB) Elemental Analysis Report (114 KB)

Handling Instructions (2659 KB)

References

[1]. Xiao CY, et al. Negative charge at the protein kinase CK2 site enhances recognition of the SV40 large T-antigen NLS by importin: effect of conformation. FEBS Lett. 1998 Dec 4;440(3):297-301. [Content Brief]

[2]. Xiao CY, et al. An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import. FEBS Lett. 1998 Oct 9;436(3):313-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.7135 mL	3.5673 mL	7.1346 mL	17.8364 mL
	5 mM	0.1427 mL	0.7135 mL	1.4269 mL	3.5673 mL
	10 mM	0.0713 mL	0.3567 mL	0.7135 mL	1.7836 mL
	15 mM	0.0476 mL	0.2378 mL	0.4756 mL	1.1891 mL
	20 mM	0.0357 mL	0.1784 mL	0.3567 mL	0.8918 mL
	25 mM	0.0285 mL	0.1427 mL	0.2854 mL	0.7135 mL
	30 mM	0.0238 mL	0.1189 mL	0.2378 mL	0.5945 mL
	40 mM	0.0178 mL	0.0892 mL	0.1784 mL	0.4459 mL
	50 mM	0.0143 mL	0.0713 mL	0.1427 mL	0.3567 mL
	60 mM	0.0119 mL	0.0595 mL	0.1189 mL	0.2973 mL