SV40 large T antigen NLS
Based on 1 Customer Validation
SV40 large T antigen NLS is from Large T antigen residue 47 to 55, enables protein import into cell nucleus.
For research use only. We do not sell to patients.
- Purity: 99.34%
- CAS No.: 163815-24-5
- Formula: C58H104N20O18S
- Molecular Weight:1401.63
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS)[1]. T-ag NLS can enhance nuclear import, despite the fact that PK-C has been shown to inhibit nuclear import of proteins such as lamin B2[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 163815-24-5
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Appearance Solid
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Molecular Weight 1401.63
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Formula C58H104N20O18S
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Color White to off-white
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Sequence
Cys-Gly-Gly-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Val-Glu-Asp
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Sequence Shortening
CGGGPKKKRKVED
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (71.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiao CY, et al. Negative charge at the protein kinase CK2 site enhances recognition of the SV40 large T-antigen NLS by importin: effect of conformation. FEBS Lett. 1998 Dec 4;440(3):297-301. [Content Brief]
[2]. Xiao CY, et al. An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import. FEBS Lett. 1998 Oct 9;436(3):313-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7135 mL | 3.5673 mL | 7.1346 mL | 17.8364 mL |
| 5 mM | 0.1427 mL | 0.7135 mL | 1.4269 mL | 3.5673 mL | |
| 10 mM | 0.0713 mL | 0.3567 mL | 0.7135 mL | 1.7836 mL | |
| 15 mM | 0.0476 mL | 0.2378 mL | 0.4756 mL | 1.1891 mL | |
| 20 mM | 0.0357 mL | 0.1784 mL | 0.3567 mL | 0.8918 mL | |
| 25 mM | 0.0285 mL | 0.1427 mL | 0.2854 mL | 0.7135 mL | |
| 30 mM | 0.0238 mL | 0.1189 mL | 0.2378 mL | 0.5945 mL | |
| 40 mM | 0.0178 mL | 0.0892 mL | 0.1784 mL | 0.4459 mL | |
| 50 mM | 0.0143 mL | 0.0713 mL | 0.1427 mL | 0.3567 mL | |
| 60 mM | 0.0119 mL | 0.0595 mL | 0.1189 mL | 0.2973 mL |
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