1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)
  3. TLR7/8 agonist 1 dihydrochloride

TLR7/8 agonist 1 dihydrochloride 

Cat. No.: HY-103698A Purity: 98.02%
Handling Instructions

TLR7/8 agonist 1 dihydrochloride is a toll-like receptor (TLR7)/TLR8 dual-agonistic imidazoquinoline.

For research use only. We do not sell to patients.

TLR7/8 agonist 1 dihydrochloride Chemical Structure

TLR7/8 agonist 1 dihydrochloride Chemical Structure

CAS No. : 1620278-72-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 385 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 550 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1100 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1750 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2750 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

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  • References

Description

TLR7/8 agonist 1 dihydrochloride is a toll-like receptor (TLR7)/TLR8 dual-agonistic imidazoquinoline.

IC50 & Target

TLR7/TLR8[1]

In Vitro

TLR7/8 agonist 1 (Compound 5d) shows prominent immunostimulatory activities. TLR7/8 agonist 1 serves as a convenient precursor for the covalent attachment of fluorophores without significant loss of activity. TLR7/8 agonist 1 retains TLR7-agonistic activity with an EC50 of 20 nM[1]. TLR7/8 agonist 1 (Compound 1) shows substantially different agonistic potencies in human TLR7 (50 nM) and TLR8 (55 nM) primary screens[2].

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (192.72 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3127 mL 11.5636 mL 23.1273 mL
5 mM 0.4625 mL 2.3127 mL 4.6255 mL
10 mM 0.2313 mL 1.1564 mL 2.3127 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

Fresh human peripheral blood mononuclear cells (hPBMC) are used. Aliquots of PBMCs (105 cells in 100 μL/well) are stimulated for 12 h with graded concentrations of test compounds (e.g., TLR7/8 agonist 1; 0.1, 1, 10, and 100 μg/mL). Supernatants are isolated by centrifugation and are assayed in duplicates using analyte-specific multiplexed cytokine/chemokine bead array assays[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

432.39

Formula

C₂₂H₂₇Cl₂N₅

CAS No.

1620278-72-9

SMILES

NC1=NC2=CC=CC=C2C3=C1N=C(CCCC)N3CC4=CC=C(CN)C=C4.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
TLR7/8 agonist 1 dihydrochloride
Cat. No.:
HY-103698A
Quantity:

TLR7/8 agonist 1 dihydrochloride

Cat. No.: HY-103698A