Art. -Nr.: HY-N0134

Tanshinone I

Name: Tanshinone I
Brand: MedChemExpress
SKU: HY-N0134
Price: 50.00 USD
Availability: InStock
Rating: 5 (5 reviews)

(Synonyms: Tanshinone A)

5 Cited Publications

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Based on 5 publication(s) in Google Scholar

Tanshinone I is an inhibitor of type IIA human recombinant sPLA₂ (IC₅₀=11 μM) and rabbit recombinant cPLA₂ (IC₅₀=82 μM).

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Reinheit : 98.83%
CAS. Nr.: 568-73-0
Formel: C₁₈H₁₂O₃
Molecular Weight:276.29

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Tanshinone I Chemical Structure

CAS. Nr.: 568-73-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
Protokoll (1)
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Tanshinone I is an inhibitor of type IIA human recombinant sPLA₂ (IC₅₀=11 μM) and rabbit recombinant cPLA₂ (IC₅₀=82 μM).

IC₅₀ & Target

IC50: 11 μM (sPLA₂), 82 μM (cPLA₂)^[1].

Alle Phospholipase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
AGS	IC₅₀	>50 μM Compound: 6, tanshinone i	Viability of human AGS cells under normoxic conditions after 24 hrs by MTT assay Viability of human AGS cells under normoxic conditions after 24 hrs by MTT assay	[PMID: 17583950]
AGS	IC₅₀	12.5 μM Compound: 6, tanshinone i	Inhibition of HIF1 activation in human AGS cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay Inhibition of HIF1 activation in human AGS cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay	[PMID: 17583950]
Hep 3B2	EC₅₀	>10 μM Compound: 3	Cytotoxicity against human Hep3B cells deficient in p53 gene by MTT assay Cytotoxicity against human Hep3B cells deficient in p53 gene by MTT assay	[PMID: 20455578]
Hep 3B2	IC₅₀	>50 μM Compound: 6, tanshinone i	Viability of human Hep3B cells under normoxic conditions after 24 hrs by MTT assay Viability of human Hep3B cells under normoxic conditions after 24 hrs by MTT assay	[PMID: 17583950]
Hep 3B2	IC₅₀	8.51 μM Compound: 6, tanshinone i	Inhibition of HIF1 activation in human Hep3B cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay Inhibition of HIF1 activation in human Hep3B cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay	[PMID: 17583950]
HepG2	EC₅₀	>10 μM Compound: 3	Cytotoxicity against doxorubicin resistant human HepG2 cells expressing wild type p53 gene by MTT assay Cytotoxicity against doxorubicin resistant human HepG2 cells expressing wild type p53 gene by MTT assay	[PMID: 20455578]
HepG2	EC₅₀	6.3 μM Compound: 3	Cytotoxicity against human HepG2 cells expressing wild type p53 gene by MTT assay Cytotoxicity against human HepG2 cells expressing wild type p53 gene by MTT assay	[PMID: 20455578]
KB	IC₅₀	5.87 μM Compound: 1	Antiproliferative activity against human KB cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human KB cells after 72 hrs by sulforhodamine B assay	[PMID: 29294282]
MCF7	ED₅₀	0.82 μg/mL Compound: Tanshinone I	Dose required for reduction in cell growth of human breast cancer MCF-7 cell line Dose required for reduction in cell growth of human breast cancer MCF-7 cell line	[PMID: 15509181]
MCF7	IC₅₀	7.927 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 33751939]
MDA-MB-231	ED₅₀	0.75 μg/mL Compound: Tanshinone I	Dose required for reduction in cell growth of human breast cancer MDA-MB-231 cell line Dose required for reduction in cell growth of human breast cancer MDA-MB-231 cell line	[PMID: 15509181]
MDA-MB-231	IC₅₀	7.962 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 33751939]
MIA PaCa-2	IC₅₀	10.5 μM Compound: 15	Cytotoxicity against human MIAPaCa2 cells after 24 hrs by MTT assay Cytotoxicity against human MIAPaCa2 cells after 24 hrs by MTT assay	[PMID: 21775156]
PLC-PRF-5	EC₅₀	>10 μM Compound: 3	Cytotoxicity against human PLC/PRF/5 cells expressing mutant p53 gene (codon 249) by MTT assay Cytotoxicity against human PLC/PRF/5 cells expressing mutant p53 gene (codon 249) by MTT assay	[PMID: 20455578]
Vero C1008	CC₅₀	>200 μM Compound: 29	Cytotoxicity against African green monkey Vero E6 cells assessed as cell viability treated for 24 hrs by CCK8 assay Cytotoxicity against African green monkey Vero E6 cells assessed as cell viability treated for 24 hrs by CCK8 assay	[PMID: 35620927]

In Vitro

Tanshinone I inhibits PGE₂ formation from LPS-induced RAW macrophages (IC₅₀=38 μM). When Tanshinone I is added simultaneously with LPS, this compound clearly inhibits PGE2 production (IC₅₀=38 μM) at 10-100 μM. Tanshinone I also reduces PGE2 production (IC₅₀=46 μM) when added after COX-2 is fully induced. The fact that Tanshinone I inhibits PGE2 production by pre-induced COX-2 strongly suggests that this compound may directly inhibit COX-2 activity and/or affect PLA₂ activity. When Tanshinone I is incubated with two different forms of phospholipase A₂ (PLA₂), it clearly inhibits sPLA₂ (IC₅₀=11 μM) in a concentration-dependent manner. Although being less potent, Tanshinone I also inhibits cPLA₂ (IC₅₀=82 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

Tanshinone I shows antiinflammatory activity in rat carrageenan-induced paw oedema and adjuvant-induced arthritis. In order to establish the anti-inflammatory activity of Tanshinone I, the classical animal models of acute and chronic inflammation [rat carrageenan (CGN)-induced paw oedema and rat adjuvant-induced arthritis (AIA)] are employed. When Tanshinone I is orally administered, it shows significant anti-inflammatory activity against CGN-induced paw oedema (47% inhibition at 160 mg/kg), while the IC₅₀ of indomethacin is 7.1 mg/kg. In AIA, Tanshinone I gives 27% inhibition of secondary inflammation at 18 day with an oral dose of 50 mg/kg/day, whereas prednisolone (5 mg/kg/day) shows potent inhibition (65%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 568-73-0
Appearance Solid
Molecular Weight 276.29
Formel C₁₈H₁₂O₃
Color Light brown to black
SMILES

O=C(C1=C2C=CC3=C1C=CC=C3C)C(C4=C2OC=C4C)=O
Synonyms

Tanshinone A
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

All Cell Proliferation/Viability AssayFlow CytometryRT-PCRWBCell Imaging/Staining

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Journal Impact Factor

Int J Mol Sci

PLA2G2A Phospholipase Promotes Fatty Acid Synthesis and Energy Metabolism in Pancreatic Cancer Cells with K-ras Mutation. [Abstract]

2022 Oct 3;23(19):11721. PMID: 36233022

Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]

PANC-1 and AsPC-1 cells were treated with various concentrations (0.5–5 µM) of Tanshinone I for various time periods, as indicated. Cell proliferation was analyzed by MTT assay.

Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]

PANC-1 and AsPC-1 cells were incubated with 5 µM Tanshinone I for 72 h; lipid peroxidation was analyzed by flow cytometry.

Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]

PANC-1 cells were incubated with Tanshinone I (5–10 µM) for 72 h. The mRNA levels of fatty acid synthesis-related genes were quantified by qRT-PCR.

Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]

AsPC-1 cells were incubated with Tanshinone I (1–10 µM) for 72 h. FASN protein level was analyzed by western blot analysis.

Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]

PANC-1 and AsPC-1 cells were treated with 5 µM Tanshinone I and incubated for two weeks to form colonies.
Fish Shellfish Immunol

Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]

2025 Nov:166:110633. PMID: 40769268
Mol Immunol

Tanshinone I limits inflammasome activation of macrophage via docking into Syk to alleviate DSS-induced colitis in mice. [Abstract]

2024 Jul 31:173:88-98. PMID: 39088935
Life Sci Alliance

Tanshinone functions as a coenzyme that confers gain of function of NQO1 to suppress ferroptosis. [Abstract]

2022 Nov 1;6(1):e202201667. PMID: 36319062
Research Square Preprint

2024 Nov 19.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 2 mg/mL (7.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6194 mL

18.0969 mL

36.1939 mL

5 mM

0.7239 mL

3.6194 mL

7.2388 mL

10 mM

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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Protokoll

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kim SY, et al. Effects of Tanshinone I isolated from Salvia miltiorrhiza bunge on arachidonic acid metabolism and in vivo inflammatory responses. Phytother Res. 2002 Nov;16(7):616-20. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6194 mL	18.0969 mL	36.1939 mL	90.4846 mL
DMSO	5 mM	0.7239 mL	3.6194 mL	7.2388 mL	18.0969 mL

Bioz

Keywords

Tanshinone I

5 Cited Publications

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Tanshinone I

Publications Citing Use of MedChemExpress (MCE) Tanshinone I

Alle Phospholipase Isoform-spezifische Produkte anzeigen