2. Anti-infection Metabolic Enzyme/Protease
  3. Influenza Virus Endogenous Metabolite
  4. Theaflavin

Theaflavin is a suitable natural inhibitor against influenza A (H1N1) neuraminidase.

For research use only. We do not sell to patients.

CAS No. : 4670-05-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Theaflavin:

Theaflavin Related Antibodies

Top Publications Citing Use of Products

    Theaflavin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 2;17(4):1050-1060.  [Abstract]

    Cellular ROS production in HK-2 cells was measured by flow cytometry with Theaflavin (TF) (20, 50 μg/mL) treatment.

    Theaflavin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 2;17(4):1050-1060.  [Abstract]

    Cellular ROS production in HK-2 cells was measured by qRT-PCR with Theaflavin (TF) (20, 50 μg/mL) treatment.

    Theaflavin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 2;17(4):1050-1060.  [Abstract]

    Analyses of SIRT1 expression in HK-2 cells incubated with COM crystals with or without Theaflavin (TF) (20, 50 μg/mL) treatment.

    Theaflavin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 2;17(4):1050-1060.  [Abstract]

    IHC was used to analyze SIRT1 expression, and FISH to detect miR-128 expression in renal tissues treated with Theaflavin (TF) (20, 50, 100 mg/kg, i.g.).

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Theaflavin is a suitable natural inhibitor against influenza A (H1N1) neuraminidase.

    IC50 & Target

    Influenza A (H1N1) virus[1]
    Cellular Effect
    Cell Line Type Value Description References
    H9 IC50
    48 μg/mL
    Compound: 36
    Inhibition of uninfected H9 lymphocytic cell growth
    Inhibition of uninfected H9 lymphocytic cell growth
    		10.1016/0960-894X(96)00095-9
    In Vitro

    Theaflavin, found in green tea, is observed to inhibit H1N1 NA proteins strongly supported by lowest docking energy. Theaflavin is a plant product traditionally used for treatment of influenza infection. Green tea is particularly rich in polyphenolic compounds like Theaflavin. Theaflavin derivatives have shown pronounced antiviral activity. Theaflavin is found to interact with the amino acid residues like Arg118, Asp151, Asp 152, Arg193, Asp199, Asn344, and Arg430 of NA by forming hydrogen bonds[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Theaflavin Related Antibodies
    Molecular Weight

    564.49

    Formula

    C29H24O12
    CAS No.
    Appearance

    Solid

    Color

    Brown to reddish brown

    SMILES

    O=C1C(O)=CC([C@@H]2[C@H](O)CC3=C(O)C=C(O)C=C3O2)=CC4=C([C@@H]5[C@H](O)CC6=C(O)C=C(O)C=C6O5)C=C(O)C(O)=C41
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (88.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2 mg/mL (3.54 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7715 mL 8.8576 mL 17.7151 mL
    5 mM 0.3543 mL 1.7715 mL 3.5430 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 3 mg/mL (5.31 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 3 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 3 mg/mL (5.31 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 3 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.69%

    SDS (396 KB)

    COA (260 KB) HNMR (203 KB) LCMS (119 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.7715 mL 8.8576 mL 17.7151 mL 44.2878 mL
    DMSO 5 mM 0.3543 mL 1.7715 mL 3.5430 mL 8.8576 mL
    10 mM 0.1772 mL 0.8858 mL 1.7715 mL 4.4288 mL
    15 mM 0.1181 mL 0.5905 mL 1.1810 mL 2.9525 mL
    20 mM 0.0886 mL 0.4429 mL 0.8858 mL 2.2144 mL
    25 mM 0.0709 mL 0.3543 mL 0.7086 mL 1.7715 mL
    30 mM 0.0591 mL 0.2953 mL 0.5905 mL 1.4763 mL
    40 mM 0.0443 mL 0.2214 mL 0.4429 mL 1.1072 mL
    50 mM 0.0354 mL 0.1772 mL 0.3543 mL 0.8858 mL
    60 mM 0.0295 mL 0.1476 mL 0.2953 mL 0.7381 mL
    80 mM 0.0221 mL 0.1107 mL 0.2214 mL 0.5536 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Theaflavin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Theaflavin4670-05-7Influenza VirusEndogenous MetaboliteInhibitorinhibitorinhibit

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