2. Metabolic Enzyme/Protease
  3. Carbonic Anhydrase
  4. U-104

U-104 (SLC-0111) is a potent carbonic anhydrase (CA) inhibitor for CA IX and CA XII with Ki values of 45.1 nM and 4.5 nM, respectively. U-104 shows a significant delay in tumor growth in mice model.

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CAS 番号 : 178606-66-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 42 在庫あり
Solution
10 mM * 1 mL in DMSO USD 42 在庫あり
Solid
5 mg $39 在庫あり
10 mg $61 在庫あり
25 mg $125 在庫あり
50 mg $193 在庫あり
100 mg $352 在庫あり
500 mg $1375 在庫あり
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カスタマーレビュー

Based on 8 publication(s) in Google Scholar

U-104 関連抗体

Top Publications Citing Use of Products

    U-104 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2025 Oct 28;122(43):e2501825122.  [Abstract]

    Hs578T cells cultured in HCO3−/CO2-buffered medium were treated for 24 h with the NBCn1 inhibitor S0859 (10 µM), the CA IX/XII inhibitor U-104 (10 µM) or a combination of the two compounds added directly in the incubator. Cycloheximide was added the last 30 min and the expression of ERK3 was analyzed by western blotting.

    U-104 purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2025 Nov 10.  [Abstract]

    Cell proliferation experiments showed a dose-dependent effect of U104 on FTC133 cells.

    U-104 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Jan 4;15(1):5.  [Abstract]

    Cell colony formation with and without U-104 treatment (left), and bar chart of colony number (right).

    U-104 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Jan 4;15(1):5.  [Abstract]

    The survival rate of cells cultured with different concentrations of U-104 after treatment with 10 μM cisplatin was examined.

    U-104 purchased from MedChemExpress. Usage Cited in: Anal Chem. 2023 Feb 28;95(8):3940-3950.  [Abstract]

    U-104 (20, 40 mg/kg; i.p; single daily for 24 days) results in tumor growth delay in mouse xenograft models.

    U-104 purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2023 Jan 31:2023:1367938.

    U-104 (0-80 μM). Protein lysates were collected from parental cells overexpressing CA9. Transferrin receptor (TrfR) (as a marker of iron uptake) and ferritin heavy chain (FTH1) (as a marker of iron storage) were analyzed by Western blotting.

    Carbonic Anhydrase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    CA CA I CA Ⅱ CA IX CA XII CA VII CA VI CA VA CA VB CA XIII CA XIV

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    U-104 (SLC-0111) is a potent carbonic anhydrase (CA) inhibitor for CA IX and CA XII with Ki values of 45.1 nM and 4.5 nM, respectively. U-104 shows a significant delay in tumor growth in mice model[1][2].

    IC50 & Target

    CA XII

     

    CA IX

     

    Cellular Effect
    Cell Line Type Value Description References
    MDA-MB-231 IC50
    138 μM
    Compound: SLC-0111
    Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell growth incubated for 72 hrs in the presence of hypoxic condition by cytofluorimetric analysis
    Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell growth incubated for 72 hrs in the presence of hypoxic condition by cytofluorimetric analysis
    		[PMID: 38911163]
    MDA-MB-231 IC50
    18.22 μM
    Compound: SLC-0111
    Antiproliferative activity against human MDA-MB-231 cells measured after 72 hrs by cell counting kit 8 method
    Antiproliferative activity against human MDA-MB-231 cells measured after 72 hrs by cell counting kit 8 method
    		[PMID: 38894925]
    U-87MG ATCC IC50
    58 μM
    Compound: SLC-0111
    Antiproliferative activity against human U-87MG cells assessed as reduction in cell growth incubated for 72 hrs in the presence of hypoxic condition by cytofluorimetric analysis
    Antiproliferative activity against human U-87MG cells assessed as reduction in cell growth incubated for 72 hrs in the presence of hypoxic condition by cytofluorimetric analysis
    		[PMID: 38911163]
    体外実験

    U-104 (SLC-0111) is a potent exosome inhibitor[3].
    U-104 has low inhibition for CA I (Ki=5080 nM) and CA II (Ki=9640 nM)[1].
    U-104 (50 μM; for 72 hours) blocks the mesenchymal phenotype in the cancer stem cells population in hypoxia condition of 4T1 cells. U-104 (<50 μM) significantly reduces migration in a dose-dependent manner in metastatic MDA-MB-231 LM2-4Luc+ cells , with cells growing as compact colonies similar to parental MDA-MB-231 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    U-104 関連抗体
    体内実験

    U-104 (19, 38 mg/kg; daily; for 27 days) inhibits primary tumor growth in the mice implanted orthotopically with MDA-MB-231 LM2-4Luc+ cells. U-104 (19 mg/kg; daily; for 5 days) inhibits metastases formation in the 4T1 experimental metastasis mice model[1].
    U-104 (38 mg/kg; i.p.; from 11 to 27 days) significantly delays primary tumor growth and reduces cancer stem cell population in NOD/SCID mice orthotopically implanted with MDA-MB-231 LM2-4Luc+ cells[2].
    U-104 (50 mg/kg; oral gavage; continuously for 4 days and suspended for 1 day; from 10 to 30 days) shows a significant delay in tumor growth in Balb/c mice orthotopically implanted with 4T1 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    309.32

    分子式

    C13H12FN3O3S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=S(C1=CC=C(NC(NC2=CC=C(F)C=C2)=O)C=C1)(N)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : ≥ 100 mg/mL (323.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2329 mL 16.1645 mL 32.3290 mL
    5 mM 0.6466 mL 3.2329 mL 6.4658 mL
    10 mM 0.3233 mL 1.6164 mL 3.2329 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.72 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    (per animal)

    g

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.95%

    COA (246 KB) LCMS (93 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.2329 mL 16.1645 mL 32.3290 mL 80.8224 mL
    5 mM 0.6466 mL 3.2329 mL 6.4658 mL 16.1645 mL
    10 mM 0.3233 mL 1.6164 mL 3.2329 mL 8.0822 mL
    15 mM 0.2155 mL 1.0776 mL 2.1553 mL 5.3882 mL
    20 mM 0.1616 mL 0.8082 mL 1.6164 mL 4.0411 mL
    25 mM 0.1293 mL 0.6466 mL 1.2932 mL 3.2329 mL
    30 mM 0.1078 mL 0.5388 mL 1.0776 mL 2.6941 mL
    40 mM 0.0808 mL 0.4041 mL 0.8082 mL 2.0206 mL
    50 mM 0.0647 mL 0.3233 mL 0.6466 mL 1.6164 mL
    60 mM 0.0539 mL 0.2694 mL 0.5388 mL 1.3470 mL
    80 mM 0.0404 mL 0.2021 mL 0.4041 mL 1.0103 mL
    100 mM 0.0323 mL 0.1616 mL 0.3233 mL 0.8082 mL
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    U-104 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    U-104178606-66-1SLC-0111U104U 104SLC0111SLC 0111Carbonic AnhydraseCarbonate dehydrataseCAIXCAXIIexosomeCAICAII4T1MDA-MB-231LM2-4Inhibitorinhibitorinhibit

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    製品名:
    U-104
    製品番号:
    HY-13513
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