Art. -Nr.: HY-13441

(-)-Blebbistatin

Name: (-)-Blebbistatin
Brand: MedChemExpress
SKU: HY-13441
Price: 72.00 USD
Availability: InStock
Rating: 5 (29 reviews)

29 Cited Publications

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(-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II.

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Reinheit : 98.68%
CAS. Nr.: 856925-71-8
Formel: C₁₈H₁₆N₂O₂
Molecular Weight:292.33

Speicherung:

4°C, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

(-)-Blebbistatin Chemical Structure

CAS. Nr.: 856925-71-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (29)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

(-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II^[1]^[2].

IC₅₀ & Target

IC50: 0.5 to 5 μM (myosin II)^[1]

Alle Myosin Isoform-spezifische Produkte anzeigen :

MLCK

In Vitro

Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC₅₀ values ranging from 0.5 to 5 μM. Smooth muscle myosin is only poorly inhibited (IC₅₀=80 μM)^[1]. Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. It blocks the myosin heads in a products complex with low actin affinity^[2]. In culture-activated hepatic stellate cells, blebbistatin is found to change both cell morphology and function. Stellate cells become smaller, acquire a dendritic morphology and have less myosin IIA-containing stress fibres and vinculin-containing focal adhesions. Blebbistatin impairs silicone wrinkle formation, reduces collagen gel contraction and blocks endothelin-1-induced intracellular Ca²⁺ release. It promotes wound-induced cell migration^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

In Vivo

Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 856925-71-8
Appearance Solid
Molecular Weight 292.33
Formel C₁₈H₁₆N₂O₂
Color Light yellow to yellow
SMILES

CC1=CC=C(N=C(N(C2=CC=CC=C2)CC3)[C@]3(O)C4=O)C4=C1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (29)

All Cell Proliferation/Viability AssayIFWB

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Journal Impact Factor

Cell

Osr2 functions as a biomechanical checkpoint to aggravate CD8+ T cell exhaustion in tumor. [Abstract]

2024 Jun 20;187(13):3409-3426.e24. PMID: 38744281
Circulation

Novel Pan-ERR Agonists Ameliorate Heart Failure Through Enhancing Cardiac Fatty Acid Metabolism and Mitochondrial Function. [Abstract]

2024 Jan 16;149(3):227-250. PMID: 37961903
Bioact Mater

Dynamic matrix engineering promotes nascent protein deposition to drive cell migration and expedite Re-epithelization in chronic wound. [Abstract]

2025 Oct 28:56:455-467. PMID: 41216373
Cell Stem Cell

Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation. [Abstract]

2025 Apr 3;32(4):581-597.e11. PMID: 39986274
Adv Fiber Mater

2026 Feb 12.
Nat Commun

RIOK2 kinase regulates the translocation of the FADD-RIPK1-Caspase-8 complex to the ER and the cleavage of Gasdermin D to drive pyroptosis. [Abstract]

2025 Nov 17;16(1):10060. PMID: 41249793

(-)-Blebbistatin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 17;16(1):10060. [Abstract]

In vitro kinase assay of p-MYL9 protein with or without RIOK2, RIOK2(D246A), or FADD. Riok2−/− iBMDMs were transfected with plasmids encoding Riok2 or Riok2(D246A) and treated with myosin II inhibitor (-)-Blebbistatin (50 μM, 0.5 hour), then stimulated with LPS/5z-7.
Nat Commun

Promoting implant osseointegration via the osteoblast-selective β-amino acid polymer strategy. [Abstract]

2025 Aug 5;16(1):7190. PMID: 40764473
Nat Chem Biol

Adhesion GPCR-induced ectocytosis mediates intercellular GPCR signal propagation. [Abstract]

2026 Feb 13. PMID: 41688711
Theranostics

Regulation of ezrin tension by S-nitrosylation mediates non-small cell lung cancer invasion and metastasis. [Abstract]

2019 Apr 13;9(9):2555-2571. PMID: 31131053
Adv Sci (Weinh)

Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice. [Abstract]

2025 Sep 17:e00358. PMID: 40959902
Adv Sci (Weinh)

Pharmacological Perturbation of Mechanical Contractility Enables Robust Transdifferentiation of Human Fibroblasts into Neurons. [Abstract]

2022 May;9(13):e2104682. PMID: 35240008

(-)-Blebbistatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May;9(13):e2104682. [Abstract]

HFFs expressed TUJ1 and MAP2 after 7 days of Ble (20 μM) treatment.

(-)-Blebbistatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May;9(13):e2104682. [Abstract]

Immunocytochemistry of YAP subcellular localization in (−)-blebbistatin (Ble) (20 μM; 7 days)-treated human foreskin fibroblasts (HFFs) was performed.
Adv Sci (Weinh)

Heterogeneous Responses to Mechanical Force of Prostate Cancer Cells Inducing Different Metastasis Patterns. [Abstract]

2020 Jun 17;7(15):1903583. PMID: 32775149

(-)-Blebbistatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]

Fluorescence imaging of LNCaP cells treated with the F-actin inhibitor cytoskeleton B (CB), microtubule inhibitor nocodazole (NO), FAK inhibitor PF573288 (PF), ROCK inhibitor Y-27632 (Y), and myosin II (Myo-II) inhibitor (-)-blebbistatin ((-)Bl). After the cells were cultured on different substrates for 3 days, inhibitors were added and incubated for 24 h. (green, F-actin; scale bar, 25 µm).

(-)-Blebbistatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]

Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).
Cell Death Dis

Pyruvate kinase M2 regulates mitochondrial homeostasis in cisplatin-induced acute kidney injury. [Abstract]

2023 Oct 10;14(10):663. PMID: 37816709
Cell Death Dis

Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma. [Abstract]

2023 Jun 28;14(6):379. PMID: 37369706
Dev Cell

STAT3-controlled CHI3L1/SPP1 positive feedback loop demonstrates the spatial heterogeneity and immune characteristics of glioblastoma. [Abstract]

2025 Jun 23;60(12):1751-1767.e9. PMID: 39933531
Neural Regen Res

Bone marrow mesenchymal stem cells protect against cerebral amyloid angiopathy by enhancing neutrophil mitocytosis. [Abstract]

2025 Jun 19. PMID: 40536954
Cell Rep

Optogenetic mediated contractility enables reversible control of microglial morphology and migration in vivo. [Abstract]

2026 Mar 27;45(4):117150. PMID: 41903137
Anal Chem

Multifrequency Optomechanical Stiffness Measurement of Single Adherent Cells on a Solid Substrate with High Throughput. [Abstract]

2017 Oct 17;89(20):10841-10849. PMID: 28895727
Fundam Res

Pharmacological regulation of tissue fibrosis by targeting the mechanical contraction of myofibroblasts. [Abstract]

2021 Dec 23;2(1):37-47. PMID: 38933917
J Cell Biol

Membrane-bound SCF and VCAM-1 synergistically regulate the morphology of hematopoietic stem cells. [Abstract]

2021 Oct 4;220(10):e202010118. PMID: 34402812
Colloids Surf B Biointerfaces

AMOT130/YAP pathway in topography-induced BMSC osteoblastic differentiation. [Abstract]

2019 Oct 1:182:110332. PMID: 31325776
Commun Biol

2026 Jun 29. PMID: 42373903
Int Immunopharmacol

Lactylation of MYH9 and its impact on FOXO3a/Bim signaling in sepsis-induced gut-vascular barrier injury. [Abstract]

2025 Oct 30:164:115384. PMID: 40850198
Nanomedicine (Lond)

Mechanical cues modulate cellular uptake of nanoparticles in cancer via clathrin-mediated and caveolae-mediated endocytosis pathways. [Abstract]

2019 Mar;14(5):613-626. PMID: 30816057
Am J Physiol Heart Circ Physiol

A novel system for mapping regional electrical properties and characterizing arrhythmia in isolated intact rat atria. [Abstract]

2021 Aug 1;321(2):H412-H421. PMID: 34213393
bioRxiv

2026 Jun 26.
Res Sq

2026 Jan 9.
SSRN

2025 Jul 29.
Patent

US20230050888A1.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 10 mg/mL (34.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4208 mL

17.1040 mL

34.2079 mL

5 mM

0.6842 mL

3.4208 mL

6.8416 mL

10 mM

0.3421 mL

1.7104 mL

3.4208 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 1 mg/mL (3.42 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Cristina Lucas‐Lopez, et al. Absolute Stereochemical Assignment and Fluorescence Tuning of the Small Molecule Tool, (–)‐Blebbistatin.

[2]. Ponsaerts R, et al. The myosin II ATPase inhibitor blebbistatin prevents thrombin-induced inhibition of intercellularcalcium wave propagation in corneal endothelial cells. Invest Ophthalmol Vis Sci. 2008 Nov;49(11):4816-27. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4208 mL	17.1040 mL	34.2079 mL	85.5198 mL
	5 mM	0.6842 mL	3.4208 mL	6.8416 mL	17.1040 mL
	10 mM	0.3421 mL	1.7104 mL	3.4208 mL	8.5520 mL
	15 mM	0.2281 mL	1.1403 mL	2.2805 mL	5.7013 mL
	20 mM	0.1710 mL	0.8552 mL	1.7104 mL	4.2760 mL
	25 mM	0.1368 mL	0.6842 mL	1.3683 mL	3.4208 mL
	30 mM	0.1140 mL	0.5701 mL	1.1403 mL	2.8507 mL

Bioz

Keywords

(-)-Blebbistatin

29 Cited Publications

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(-)-Blebbistatin

Publications Citing Use of MedChemExpress (MCE) (-)-Blebbistatin

Alle Myosin Isoform-spezifische Produkte anzeigen