Cat. No.: HY-N0797

(20S)-Protopanaxadiol

(Synonyms: 20-Epiprotopanaxadiol; 20(S)-APPD)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

20S-protopanaxadiol (aPPD) is a metabolite of ginseng saponins, inhibits Akt activity and induces apoptosis in various tumor cells.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 30636-90-9
Formula: C₃₀H₅₂O₃
Molecular Weight:460.73

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

(20S)-Protopanaxadiol Chemical Structure

CAS No.: 30636-90-9

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Top Publications Citing

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

20S-protopanaxadiol (aPPD) is a metabolite of ginseng saponins, inhibits Akt activity and induces apoptosis in various tumor cells^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	99.89 μM Compound: PPD	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21216145]
A549	IC₅₀	99.89 μM Compound: PPD	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 22342101]
BMMC	IC₅₀	11.8 μM Compound: PPD	Anti-osteoclastogenic activity in C57/BL6 mouse BMMC cells assessed as inhibition of RANKL-induced osteoclast differentiation incubated for 5 to 7 days in presence of M-CSF by TRAP staining based microscopic analysis relative to control Anti-osteoclastogenic activity in C57/BL6 mouse BMMC cells assessed as inhibition of RANKL-induced osteoclast differentiation incubated for 5 to 7 days in presence of M-CSF by TRAP staining based microscopic analysis relative to control	[PMID: 37597216]
COLO 205	IC₅₀	49.16 μM Compound: PPD	Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay	[PMID: 21216145]
DU-145	IC₅₀	60.41 μM Compound: PPD	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 cells after 72 hrs by MTT assay	[PMID: 21216145]
Hep 3B2	IC₅₀	14.69 μM Compound: PPD	Cytotoxicity against human Hep3B cells after 72 hrs by MTT assay Cytotoxicity against human Hep3B cells after 72 hrs by MTT assay	[PMID: 22342101]
HepG2	IC₅₀	39.42 μM Compound: PPD	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 21216145]
HepG2	IC₅₀	45.67 μM Compound: 3	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 26420067]
HL-60	IC₅₀	15.53 μM Compound: 3	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 26420067]
HL-60	IC₅₀	27.06 μM Compound: PPD	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 21216145]
HUVEC	EC₅₀	2.16 μg/mL Compound: 1	Antiproliferative activity against HUVEC cells after 72 hrs Antiproliferative activity against HUVEC cells after 72 hrs	[PMID: 18271556]
LLC-PK1	IC₅₀	36.7 μM Compound: PPD	Cytotoxicity against pig LLC-PK1 cells after 4 hrs by MTT assay Cytotoxicity against pig LLC-PK1 cells after 4 hrs by MTT assay	[PMID: 25453798]
MCF7	IC₅₀	6.62 μM Compound: PPD	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 21216145]
MDA-MB-231	IC₅₀	5.9 μM Compound: PPD	Antiproliferative activity against human MDA-MB-231 cells after 4 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 4 hrs by MTT assay	[PMID: 25453798]
SF-188	IC₅₀	24 μM Compound: 1	Cytotoxicity against human SF188 cells after 24 hrs Cytotoxicity against human SF188 cells after 24 hrs	[PMID: 17261067]
U-87MG ATCC	IC₅₀	36.74 μM Compound: PPD	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	[PMID: 22342101]
U-87MG ATCC	IC₅₀	38 μM Compound: 1	Cytotoxicity against human U87MG cells after 24 hrs Cytotoxicity against human U87MG cells after 24 hrs	[PMID: 17261067]

In Vitro

20S-protopanaxadiol treatment upregulates the flotillin-1 level in the rafts of N2a cells to 142.91±10.71% of the control. 20S-protopanaxadiol can have an opposite effect on raft resident protein flotillin-1, depending on the cell type^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 30636-90-9
Appearance Solid
Molecular Weight 460.73
Formula C₃₀H₅₂O₃
Color White to off-white
SMILES

C[C@]([C@@](C[C@H]1O)([H])[C@]2(CC[C@@H]3O)C)(CC[C@@]2([H])C3(C)C)[C@]4([C@@]1([H])[C@]([C@@](C)(O)CC/C=C(C)/C)([H])CC4)C
Synonyms

20-Epiprotopanaxadiol; 20(S)-APPD
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

All IHCWBIn Vivo Efficacy StudyBio/Physico-chemical Assay

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Journal Impact Factor

Acta Pharmacol Sin

Ginsenoside (20)S-APPT induces ferroptosis in hepatocellular carcinoma and cholangiocarcinoma by targeting FSP1. [Abstract]

2025 Jun 23. PMID: 40550962
Int Immunopharmacol

20(S)-PPD-HSA NPs enhance the recovery of intramedullary and extramedullary hematopoiesis in cyclophosphamide-treated mice through activation of the FGFR1/ERK pathway. [Abstract]

2025 Dec 23:170:116073. PMID: 41443104
Ren Fail

Analysis of potential targets of 20S-protopanaxadiol on diabetic nephropathy based on network pharmacology, molecular docking, and experimental validation. [Abstract]

2025 Dec;47(1):2526688. PMID: 40957644

(20S)-Protopanaxadiol purchased from MedChemExpress. Usage Cited in: Ren Fail. 2025 Dec;47(1):2526688. [Abstract]

Representative immunohistochemical staining of TGF-β1 in kidney sections treated with (20S)-Protopanaxadiol (PPD) (20 mg/kg, i.g.).

(20S)-Protopanaxadiol purchased from MedChemExpress. Usage Cited in: Ren Fail. 2025 Dec;47(1):2526688. [Abstract]

Western blot analysis of TGF-β1, α-smooth muscle actin (α-SMA), Smad3, phosphorylated Smad3 (p-Smad3), and Smad4 expression in DN mice after (20S)-Protopanaxadiol (PPD) (20 mg/kg) intragastric administration.

(20S)-Protopanaxadiol purchased from MedChemExpress. Usage Cited in: Ren Fail. 2025 Dec;47(1):2526688. [Abstract]

Body weight following 3-week (20S)-Protopanaxadiol (PPD) (20 mg/kg, i.g.). administration.

(20S)-Protopanaxadiol purchased from MedChemExpress. Usage Cited in: Ren Fail. 2025 Dec;47(1):2526688. [Abstract]

Serum Creatinine levels of mice in each group after (20S)-Protopanaxadiol (PPD) (20 mg/kg) intragastric administration.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (108.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1705 mL

10.8523 mL

21.7047 mL

5 mM

0.4341 mL

2.1705 mL

4.3409 mL

10 mM

0.2170 mL

1.0852 mL

2.1705 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. iu Y, Yang G, Bu X, et al. Cell-type-specific regulation of raft-associated Akt signaling. Cell Death Dis. 2011;2(4):e145. Published 2011 Apr 14. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1705 mL	10.8523 mL	21.7047 mL	54.2617 mL
	5 mM	0.4341 mL	2.1705 mL	4.3409 mL	10.8523 mL
	10 mM	0.2170 mL	1.0852 mL	2.1705 mL	5.4262 mL
	15 mM	0.1447 mL	0.7235 mL	1.4470 mL	3.6174 mL
	20 mM	0.1085 mL	0.5426 mL	1.0852 mL	2.7131 mL
	25 mM	0.0868 mL	0.4341 mL	0.8682 mL	2.1705 mL
	30 mM	0.0723 mL	0.3617 mL	0.7235 mL	1.8087 mL
	40 mM	0.0543 mL	0.2713 mL	0.5426 mL	1.3565 mL
	50 mM	0.0434 mL	0.2170 mL	0.4341 mL	1.0852 mL
	60 mM	0.0362 mL	0.1809 mL	0.3617 mL	0.9044 mL
	80 mM	0.0271 mL	0.1357 mL	0.2713 mL	0.6783 mL
	100 mM	0.0217 mL	0.1085 mL	0.2170 mL	0.5426 mL

Keywords

(20S)-Protopanaxadiol

3 Cited Publications

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(20S)-Protopanaxadiol

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Publications Citing Use of MedChemExpress (MCE) (20S)-Protopanaxadiol

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(20S)-Protopanaxadiol Related Classifications

HY-N0797 Related Classifications