2. Metabolic Enzyme/Protease
  3. Proteasome
  4. Ac-RLR-AMC

Ac-RLR-AMC  (Synonyms: Ac-Arg-Leu-Arg-AMC)

Cat. No.: HY-135070 Purity: 98.91%
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Handling Instructions Technical Support

Ac-RLR-AMC (Ac-Arg-Leu-Arg-AMC) is a fluorogenic substrate for the 26S proteasome (Ex/Em: 380/440-460 nm). AMC is released upon cleavage, and its fluorescence can be used to quantify the trypsin-like activity of 26S proteasomes.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-RLR-AMC

Ac-RLR-AMC Chemical Structure

CAS No. : 929903-87-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
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100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of Ac-RLR-AMC:

Ac-RLR-AMC Related Antibodies

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Description

Ac-RLR-AMC (Ac-Arg-Leu-Arg-AMC) is a fluorogenic substrate for the 26S proteasome (Ex/Em: 380/440-460 nm). AMC is released upon cleavage, and its fluorescence can be used to quantify the trypsin-like activity of 26S proteasomes[1].

Molecular Weight

642.75

Formula

C30H46N10O6
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ac-Arg-Leu-Arg-AMC
Sequence Shortening

Ac-RLR-AMC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (155.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5558 mL 7.7791 mL 15.5581 mL
5 mM 0.3112 mL 1.5558 mL 3.1116 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5558 mL 7.7791 mL 15.5581 mL 38.8954 mL
5 mM 0.3112 mL 1.5558 mL 3.1116 mL 7.7791 mL
10 mM 0.1556 mL 0.7779 mL 1.5558 mL 3.8895 mL
15 mM 0.1037 mL 0.5186 mL 1.0372 mL 2.5930 mL
20 mM 0.0778 mL 0.3890 mL 0.7779 mL 1.9448 mL
25 mM 0.0622 mL 0.3112 mL 0.6223 mL 1.5558 mL
30 mM 0.0519 mL 0.2593 mL 0.5186 mL 1.2965 mL
40 mM 0.0389 mL 0.1945 mL 0.3890 mL 0.9724 mL
50 mM 0.0311 mL 0.1556 mL 0.3112 mL 0.7779 mL
60 mM 0.0259 mL 0.1297 mL 0.2593 mL 0.6483 mL
80 mM 0.0194 mL 0.0972 mL 0.1945 mL 0.4862 mL
100 mM 0.0156 mL 0.0778 mL 0.1556 mL 0.3890 mL
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Ac-RLR-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-RLR-AMC929903-87-7Ac-Arg-Leu-Arg-AMCProteasome26S proteasomeInhibitorinhibitorinhibit

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