ALV2
Based on 1 Customer Validation
ALV2 is a potent and selective Helios molecular glue degrader. ALV2 binds CRBN, with an IC50 of 0.57 μM. Helios is the zinc-finger transcription factor that can maintain a stable Treg cell phenotype in the inflammatory tumor microenvironment.
For research use only. We do not sell to patients.
- Purity: 98.05%
- CAS No.: 2438124-95-7
- Formula: C26H26ClN5O5
- Molecular Weight:523.97
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cereblon |
ALV2 (10 nM-100 μM) induces CRBN-Helios dimerization in the TR-FRET assay[1].
ALV2 (0.1-10 μM) preferentially promotes Helios degradation in Jurkat cells[1].
ALV2 (1 μM; pretreated for 18 h) promotes IL-2 secretion in Jurkat cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CrbnI391V/I391V mice
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Dosage:100 mg/kg
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Administration:I.p. twice daily for 7 days
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Result:Reduced Helios, but not Ikaros, levels in splenic CD4+FoxP3+ Treg cells.
Chemical Information
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CAS No. 2438124-95-7
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Appearance Solid
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Molecular Weight 523.97
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Formula C26H26ClN5O5
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Color White to yellow
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SMILES
O=C(NC(C)(C1=CC=CC(NC2=CC(N(C(CC3)C(NC3=O)=O)C2=O)=O)=C1)C)NC4=CC=C(C)C(Cl)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 160 mg/mL (305.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 4 mg/mL (7.63 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 4 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 4 mg/mL (7.63 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 4 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9085 mL | 9.5425 mL | 19.0851 mL | 47.7127 mL |
| 5 mM | 0.3817 mL | 1.9085 mL | 3.8170 mL | 9.5425 mL | |
| 10 mM | 0.1909 mL | 0.9543 mL | 1.9085 mL | 4.7713 mL | |
| 15 mM | 0.1272 mL | 0.6362 mL | 1.2723 mL | 3.1808 mL | |
| 20 mM | 0.0954 mL | 0.4771 mL | 0.9543 mL | 2.3856 mL | |
| 25 mM | 0.0763 mL | 0.3817 mL | 0.7634 mL | 1.9085 mL | |
| 30 mM | 0.0636 mL | 0.3181 mL | 0.6362 mL | 1.5904 mL | |
| 40 mM | 0.0477 mL | 0.2386 mL | 0.4771 mL | 1.1928 mL | |
| 50 mM | 0.0382 mL | 0.1909 mL | 0.3817 mL | 0.9543 mL | |
| 60 mM | 0.0318 mL | 0.1590 mL | 0.3181 mL | 0.7952 mL | |
| 80 mM | 0.0239 mL | 0.1193 mL | 0.2386 mL | 0.5964 mL | |
| 100 mM | 0.0191 mL | 0.0954 mL | 0.1909 mL | 0.4771 mL |
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