Anti-Mouse IL-15 Antibody (AIO.3)
Based on 1 Customer Validation
Anti-Mouse IL-15 Antibody (AIO.3) is a rat-derived IgG2a λ type antibody inhibitor, targeting to mouse IL-15. Anti-Mouse IL-15 Antibody (AIO.3) can neutralize IL-15. Anti-Mouse IL-15 Antibody (AIO.3) can be used for the researches of cancer, infection and inflammation, such as hepatitis virus infection and esophagitis.
For research use only. We do not sell to patients.
- Purity: ≥95.0%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
IL-15
In Vitro
The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Anti-Mouse IL-15 Antibody (AIO.3) (4 mg/kg, i.v., twice a week) is effective in preserving coagulation factor VIII expression and has broad anti-inflammatory effects in adeno-associated viral (AAV)-transduced hemophilia A mice[2].
Anti-Mouse IL-15 Antibody (AIO.3) (300 µg/mouse, i.p., 3 times a week) ameliorates podocyte injury and glomerulosclerosis in Adriamycin (HY-121309)-induced focal segmental glomerulosclerosis mice models[3].
Anti-Mouse IL-15 Antibody (AIO.3) (100 µg, i.v., at days 0, 4, 7, 11, 14, 18) reduces esophageal eosinophilia in experimental eosinophilic esophagitis mice models[4].
Anti-Mouse IL-15 Antibody (AIO.3) (1 mg/kg, i.p., daily from day 5 to 11) reduces therapeutic effect in response to anti-CD40 agonist in intravesical tumor mice models[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse hepatitis virus infected mice models[1]
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Dosage:25 µg/mouse
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Administration:Intraperitoneally injection, daily from from day -1 to day 2 of the infection
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Result:Efficiently blunted the exaggerated population expansion of group 1 ILCs in Myd88-cKO PPs.
Led to a reduced frequency and absolute number of group 1 ILCs in Myd88-sufficient mice and diminished the ILC3 population both in Myd88-sufficient PPs and in Myd88-cKO PPs.
Disinhibited viral replication in Myd88-cKO mice.
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Animal Model:Male hemophilia A mice with targeted deletion of F8 exon 16 (6-8 weeks)[2]
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Dosage:4 mg/kg
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Administration:Intravenously injection, twice a week, combined with rapamycin (HY-10219)
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Result:Preserved hFVIII expression.
Resulted in significantly higher average FVIII activity by week 16.
Had modestly increased NK cell frequencies and moderately decreased CD8+ Teff cells.
Increased expression of CD206.
Had elevated p-eIF2a and IFN-γ transcripts.
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Animal Model:Adriamycin (HY-121309)-induced focal segmental glomerulosclerosis mice models[3]
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Dosage:300 µg /mouse
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Administration:Intraperitoneally injection, 3 times a week
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Result:Significantly inhibited renal CD8+ TRM cell formation and activation.
Showed lower UACR and fewer glomerular and podocyte injuries.
Recovered the expressions of nephrin and podocin.
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Animal Model:Experimental eosinophilic esophagitis mice models[4]
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Dosage:100 µg
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Administration:Intravenously injection , at days 0, 4, 7, 11, 14, 18
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Result:Reduced esophageal eosinophilia.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vivo IL-15 neutralization; in vitro IL-15 neutralization
Verified Bioactivity
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse IL-15 Antibody (AIO.3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Gil-Cruz C, et al. Fibroblastic reticular cells regulate intestinal inflammation via IL-15-mediated control of group 1 ILCs. Nat Immunol. 2016 Dec;17(12):1388-1396. [Content Brief]
[2]. Butterfield JSS, et al. IL-15 blockade and rapamycin rescue multifactorial loss of factor VIII from AAV-transduced hepatocytes in hemophilia A mice. Mol Ther. 2022 Dec 7;30(12):3552-3569. [Content Brief]
[3]. Li L, et al. Targeting tissue-resident memory CD8+ T cells in the kidney is a potential therapeutic strategy to ameliorate podocyte injury and glomerulosclerosis. Mol Ther. 2022 Aug 3;30(8):2746-2759. [Content Brief]
[4]. Vicari AP, et al. Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis. MAbs. 2017 Aug/Sep;9(6):927-944. [Content Brief]
[5]. Wong JL, et al. IL-15 synergizes with CD40 agonist antibodies to induce durable immunity against bladder cancer. Proc Natl Acad Sci U S A. 2023 Aug 29;120(35):e2306782120. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse IL-15 Antibody (AIO.3) Related Classifications
HY-P990223 Related Classifications
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