Antitumor agent-119
Based on 1 Customer Validation
Antitumor agent-119 (compound 13K) is a 2-benzoxazolyl hydrazone derivative with anticancer activities. Antitumor agent-119 inhibits the cell growth of Butkitt, CCRF-CEM, HeLa, and HT-29 with IC50 values of 30 nM, 140 nM, 100 nM, and 40 nM, respectively.
For research use only. We do not sell to patients.
- Purity: 98.03%
- CAS No.: 913169-79-6
- Formula: C18H14N4O
- Molecular Weight:302.34
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Burkitts lymphoma cell | IC50 |
0.03 μM
Compound: E-13k, NSC-693638
|
Antiproliferative activity against Burkitt cells by MTT assay
Antiproliferative activity against Burkitt cells by MTT assay
|
[PMID: 17034140] |
| CCRF-CEM | IC50 |
0.14 μM
Compound: E-13k, NSC-693638
|
Antiproliferative activity against CCRF-CEM cells by MTT assay
Antiproliferative activity against CCRF-CEM cells by MTT assay
|
[PMID: 17034140] |
| CCRF-CEM | IC50 |
0.14 μM
Compound: 13K
|
Cytotoxicity against Homo sapiens (human) CCRF-CEM cells assessed as growth inhibition
Cytotoxicity against Homo sapiens (human) CCRF-CEM cells assessed as growth inhibition
|
10.1007/s00044-010-9510-3 |
| HeLa | IC50 |
0.1 μM
Compound: E-13k, NSC-693638
|
Antiproliferative activity against HeLa cells by SRB assay
Antiproliferative activity against HeLa cells by SRB assay
|
[PMID: 17034140] |
| HeLa | IC50 |
0.1 μM
Compound: 13K
|
Cytotoxicity against Homo sapiens (human) HeLa cells assessed as growth inhibition
Cytotoxicity against Homo sapiens (human) HeLa cells assessed as growth inhibition
|
10.1007/s00044-010-9510-3 |
| HT-29 | IC50 |
0.04 μM
Compound: E-13k, NSC-693638
|
Antiproliferative activity against HT29 cells by SRB assay
Antiproliferative activity against HT29 cells by SRB assay
|
[PMID: 17034140] |
| HT-29 | IC50 |
0.04 μM
Compound: 13K
|
Cytotoxicity against Homo sapiens (human) HT-29 cells assessed as growth inhibition
Cytotoxicity against Homo sapiens (human) HT-29 cells assessed as growth inhibition
|
10.1007/s00044-010-9510-3 |
Chemical Information
-
CAS No. 913169-79-6
-
Appearance Solid
-
Molecular Weight 302.34
-
Formula C18H14N4O
-
Color Off-white to yellow
-
SMILES
C/C(C1=CC2=C(C=CC=C2)C=N1)=N\NC3=NC4=CC=CC=C4O3
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (330.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3075 mL | 16.5377 mL | 33.0753 mL | 82.6884 mL |
| 5 mM | 0.6615 mL | 3.3075 mL | 6.6151 mL | 16.5377 mL | |
| 10 mM | 0.3308 mL | 1.6538 mL | 3.3075 mL | 8.2688 mL | |
| 15 mM | 0.2205 mL | 1.1025 mL | 2.2050 mL | 5.5126 mL | |
| 20 mM | 0.1654 mL | 0.8269 mL | 1.6538 mL | 4.1344 mL | |
| 25 mM | 0.1323 mL | 0.6615 mL | 1.3230 mL | 3.3075 mL | |
| 30 mM | 0.1103 mL | 0.5513 mL | 1.1025 mL | 2.7563 mL | |
| 40 mM | 0.0827 mL | 0.4134 mL | 0.8269 mL | 2.0672 mL | |
| 50 mM | 0.0662 mL | 0.3308 mL | 0.6615 mL | 1.6538 mL | |
| 60 mM | 0.0551 mL | 0.2756 mL | 0.5513 mL | 1.3781 mL | |
| 80 mM | 0.0413 mL | 0.2067 mL | 0.4134 mL | 1.0336 mL | |
| 100 mM | 0.0331 mL | 0.1654 mL | 0.3308 mL | 0.8269 mL |
Powered by Bioz