1. Click Chemistry
  2. Alkynes
  3. AP-C5

AP-C5 displays selective inhibition of guanosine 3′,5′-cyclic monophosphate (cGMP)-dependent protein kinase II (cGKII) with a pIC50 of 7.2, which can be used for the research of diarrheal disease. AP-C5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

AP-C5 Chemical Structure

AP-C5 Chemical Structure

CAS No. : 2234272-10-5

Size Price Stock Quantity
5 mg USD 65 In-stock
10 mg USD 110 In-stock
25 mg USD 220 In-stock
50 mg USD 360 In-stock
100 mg USD 580 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AP-C5 displays selective inhibition of guanosine 3′,5′-cyclic monophosphate (cGMP)-dependent protein kinase II (cGKII) with a pIC50 of 7.2, which can be used for the research of diarrheal disease[1]. AP-C5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC50 & Target

pIC50: 7.2 (cGKII)[1]

In Vitro

AP-C5 shows potent inhibition of cGMP-dependent cGKII-mediated protein phosphorylation and effective inhibition of cGMP-dependent, CFTR-mediated anion secretion in intestinal tissue[1].
AP-C5 potentiates cAMP signaling by PDE inhibition[1].
AP-C5 (20 μM) partially blocks the heat-stable toxin (STa)-mediated short-circuit current (Isc) response in mouse ileum[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

275.31

Formula

C16H13N5

CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

C#CCNC1=NC=CC(C2=CC=C(N3C=CN=C3)C=C2)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (90.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6323 mL 18.1613 mL 36.3227 mL
5 mM 0.7265 mL 3.6323 mL 7.2645 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6323 mL 18.1613 mL 36.3227 mL 90.8067 mL
5 mM 0.7265 mL 3.6323 mL 7.2645 mL 18.1613 mL
10 mM 0.3632 mL 1.8161 mL 3.6323 mL 9.0807 mL
15 mM 0.2422 mL 1.2108 mL 2.4215 mL 6.0538 mL
20 mM 0.1816 mL 0.9081 mL 1.8161 mL 4.5403 mL
25 mM 0.1453 mL 0.7265 mL 1.4529 mL 3.6323 mL
30 mM 0.1211 mL 0.6054 mL 1.2108 mL 3.0269 mL
40 mM 0.0908 mL 0.4540 mL 0.9081 mL 2.2702 mL
50 mM 0.0726 mL 0.3632 mL 0.7265 mL 1.8161 mL
60 mM 0.0605 mL 0.3027 mL 0.6054 mL 1.5134 mL
80 mM 0.0454 mL 0.2270 mL 0.4540 mL 1.1351 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
AP-C5
Cat. No.:
HY-130530
Quantity:
MCE Japan Authorized Agent: